Effect of salts on the excited state of pyranine as determined by steady-state fluorescence

Barnadas‐Rodríguez, Ramon; Estelrich, Joan

doi:10.1016/j.jphotochem.2008.04.003

Cited by 18 publications

(24 citation statements)

References 22 publications

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“…However, the emission of fluorescence intensity depends on dye concentration, amount of liquid, excited source, salt, and P.H. [20][21][22].Therefore, the experiment was performed with the same parameters for the emission to indicate only the existence of fluorescence liquid. This helps differentiate the contact and non-contact areas easily.…”

Section: Pyranine Solutionmentioning

confidence: 99%

In Situ Rubber-Wheel Contact on Road Surface Using Ultraviolet-Induced Fluorescence Method

2020

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This study examines the relationship between rubber-wheel and the contact area on the road surface. Ultraviolet-induced fluorescence microscopy was used to observe and measure the contact parts with pyranine as a dye solution. The high sensitivity to U.V. light makes it easy to distinguish contact and non-contact regions on a very small scale. The experiment was conducted in static and dynamic conditions to identify its influence on the apparent contact area of rubber-wheel and road surface. The in-situ observation of the contact area was captured and recorded using a high-speed digital camera with 1-inch a CMOS (complementary metal oxide semiconductor) sensor. Additionally, the contact area between rubber-wheel and road surface was measured using an analyzing software. The results show differences in static and dynamic contact conditions based on the operating parameters.

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Section: Pyranine Solutionmentioning

confidence: 99%

In Situ Rubber-Wheel Contact on Road Surface Using Ultraviolet-Induced Fluorescence Method

2020

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“…In the absence of CHAPSO and at pH 5 the main form of HPTS * is the deprotonated species since the prototropic equilibrium of the photoacid has a very low pK * a , of about 1.4. 28,29 Upon increasing the concentration of CHAPSO, the emission spectrum of HPTS showed two types of modifications. First, there was a gradual decrease of the intensity of the RO -* peak and an increase of the ROH * peak.…”

Section: Effect Of Chapso On the Spectral Properties Of The Excited Smentioning

confidence: 99%

Steroidal Surfactants: Detection of Premicellar Aggregation, Secondary Aggregation Changes in Micelles, and Hosting of a Highly Charged Negative Substance

Barnadas‐Rodríguez

Cladera

2015

Langmuir

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CHAPSO and CHAPS are zwitterionic surfactants derived from bile salts which are usually employed in protein purification and for the preparation of liposomes and bicelles. Despite their spread use, there are significant discrepancies on the critical concentrations that determine their aggregation behavior. In this work, we study the interaction between these surfactants with the negative fluorescent dye pyranine (HPTS) by absorbance, fluorescence, and infrared spectrometry to establish their concentration-dependent aggregation. For the studied surfactants, we detect three critical concentrations showing their concentration-dependent presence as a monomeric form, premicellar aggregates, micelles, and a second type of micelle in aqueous medium. The nature of the interaction of HPTS with the surfactants was studied using analogues of their tails and the negative bile salt taurocholate (TC) as reference for the sterol ring. The results indicate that the chemical groups involved are the hydroxyl groups of the polar face of the sterol ring and the sulfonate groups of the dye. This interaction causes not only the incorporation of the negative dye in CHAPSO and CHAPS micelles but also its association with their premicellar aggregates. Surprisingly, this hosting behavior for a negative charged molecule was also detected for the negative bile salt TC, bypassing, in this way, the electrostatic repulsion between the guest and the host.

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“…HPTS is a non-toxic photoacid [17] with ground state pK a values ranging from 7.3 -8.1 [18,19], and its excited state pK a has been reported as both 0.32 [20] and 1.4 [19,21]. It is widely used in probes of proton transfer kinetics [20, 22 -29].…”

Section: à/3àmentioning

confidence: 99%

Electrochemical and Optical Behavior of 8‐Hydroxypyrene‐1,3,6‐trisulfonic Acid at Optically Transparent Electrodes

2010

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The objective of this work is to elucidate the electrochemical and corresponding optical properties of 8-hydroxypyrene-1,3,6-trisulfonic acid (HPTS), using optically transparent electrodes, thereby deducing its usefulness as a model compound for spectroelectrochemical sensor development. Three pH levels were tested to determine optimal solution conditions for optical signal modulation. The electrolysis of HPTS follows an ECE mechanism, presumably resulting in the formation of a dihydroxy/dione derivative, and modulates the optical response at 405 and 460 nm wavelengths for pH 5 solutions. HPTS is a good candidate for spectroelectrochemical sensor research.

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Effect of salts on the excited state of pyranine as determined by steady-state fluorescence

Cited by 18 publications

References 22 publications

In Situ Rubber-Wheel Contact on Road Surface Using Ultraviolet-Induced Fluorescence Method

In Situ Rubber-Wheel Contact on Road Surface Using Ultraviolet-Induced Fluorescence Method

Steroidal Surfactants: Detection of Premicellar Aggregation, Secondary Aggregation Changes in Micelles, and Hosting of a Highly Charged Negative Substance

Electrochemical and Optical Behavior of 8‐Hydroxypyrene‐1,3,6‐trisulfonic Acid at Optically Transparent Electrodes

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