Effect of salts formed by neutralization for the enzymatic hydrolysis of cellulose and acetone–butanol–ethanol fermentation

Yang, Ming; Wang, Jia; Nan, Yufei; Zhang, Junhua; Li, Liyun; Liu, Guozhen; Vepsäläinen, Jouko; Kuittinen, Suvi; Pappinen, Ari

doi:10.1039/c9ra06869d

Cited by 10 publications

(6 citation statements)

References 30 publications

(34 reference statements)

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“…The high amount of ash in the pretreated biomass can led to increased concentration of salts during subsequent saccharification and fermentation trials, which could negatively affect the glucose release and inhibit the fermentation process. 28 Most initial ash composed of Na (5.2% w/w), followed by Mg (1.4% w/w) and Ca (1.2% w/w), reflected the ash composition of seawater. Only 5.1% of Na and 24.1% of Mg were recovered in the pretreated biomass, while the levels of other ions remained substantially higher.…”

Section: ■ Results and Discussionmentioning

confidence: 95%

“…To gain further insight on the effect of pretreatment on tunicate biomass, we compared ash composition of untreated biomass (24.2% w/w) to that of the T2 sample (32.3% w/w), focusing on Ca, Mg, Na, P, and Si (Table ). The high amount of ash in the pretreated biomass can led to increased concentration of salts during subsequent saccharification and fermentation trials, which could negatively affect the glucose release and inhibit the fermentation process . Most initial ash composed of Na (5.2% w/w), followed by Mg (1.4% w/w) and Ca (1.2% w/w), reflected the ash composition of seawater.…”

Section: Resultsmentioning

confidence: 99%

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Second-Generation Biofuel Production from the Marine Filter Feeder Ciona intestinalis

Hrůzová

Μάτσακας

Karnaouri

et al. 2020

ACS Sustainable Chem. Eng.

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Biofuels are essential for transitioning to a sustainable society. This switch can be achieved by introducing novel feedstocks and technologies for efficient and economically feasible biofuel production. Second-generation biofuels are particularly advantageous, as they are produced from nonedible lignocellulosic biomass derived primarily from agricultural byproducts. Ciona intestinalis, a marine filter feeder, is cultivated to produce fish feed from the invertebrate's inner tissue body. This process generates also vast amounts of a renewable side stream, namely the tunicate's external cellulose-rich tunic. The aim of the present study was to evaluate the potential of the C. intestinalis tunic as a novel feedstock for bioethanol production. For this purpose, organosolv fractionation of the tunic was optimized to increase cellulose content. Enzymatic saccharification of the pretreated biomass was assessed to identify the most promising materials, which were subsequently utilized as carbon source in fermentation trials. Under optimal conditions, a titer of 38.7 g/L of ethanol, with a yield of 78.3% of the maximum theoretical, was achieved. To the best of our knowledge, this is the first report whereby organosolv pretreated tunic biomass is valorized toward bioethanol production; the current work paves the way for incorporating tunicates in bioconversion processes for the generation of biofuels and other biobased chemicals.

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Section: ■ Results and Discussionmentioning

confidence: 95%

Section: Resultsmentioning

confidence: 99%

Second-Generation Biofuel Production from the Marine Filter Feeder Ciona intestinalis

Hrůzová

Μάτσακας

Karnaouri

et al. 2020

ACS Sustainable Chem. Eng.

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show abstract

“…One possible solution is the use of a halophilic microorganism for the fermentation stage, this would allow use of saline water, the most abundant source on the planet, in place of freshwater. Saline water may not be suitable for the prior biomass pretreatment and enzymatic hydrolysis steps, which are sensitive to salinity deviations [40]. Despite this, its use in the fermentation step would reduce the overall freshwater demand of the process and hence production costs [41].…”

Section: Water Demandmentioning

confidence: 99%

Cell Factories for Industrial Production Processes: Current Issues and Emerging Solutions

et al. 2020

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Despite all the progresses made by metabolic engineering, still only a few biotechnological processes are running at an industrial level. In order to boost the biotechnological sector, integration strategies as well as long-term views are needed. The aim of the present review is to identify the main drawbacks in biotechnological processes, and to propose possible solutions to overcome the issues in question. Novel cell factories and bioreactor design are discussed as possible solutions. In particular, the following microorganisms: Yarrowia lipolytica, Trichosporon oleaginosus, Ustilago cynodontis, Debaryomyces hansenii along with sequential bioreactor configurations are presented as possible cell factories and bioreactor design solutions, respectively.

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“…Figure 2 depicts route 1 block diagram 1, and Figure 3 shows route 2, including upstream and downstream units. During hydrolysis, cellulose is transformed into fermentable sugars by the cellulase enzyme [48]. This enzyme was assumed to contain endoglucanases, exoglucanases, and b-glucosidase for polymer size reduction, chemical hydrolysis, and glucose production [49].…”

Section: Processes Descriptionmentioning

confidence: 99%

Technical, Environmental, and Process Safety Assessment of Acetone-Butanol-Ethanol Fermentation of Cassava Residues

2022

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The North-Colombian region has enormous potential for producing bioproducts and bioenergy from agricultural residues. Yet, scaling bioproducts and bioenergy to industrial practice requires further investigation, especially for environmental impact minimization and improved process safety. This work assesses two alternatives for valorizing cassava residues via acetone, butanol, and ethanol (ABE) fermentation. Two ABE fermentation routes are assessed. In Route 1, pretreatment and purification involve dilute-acid pretreatment and multi-effect distillation and decantation operations, while Route 2 includes steam explosion and reactive distillation. Hazard Identification and Risk Assessment (HIRA) and Waste Reduction Algorithm (WAR) were applied to assess ABE fermentation. Simulation results indicate butanol yields of 0.10–0.12 kg/kg feedstock and net energy ratio (NER) <1. Route 2 shows the highest total output of Potential Environmental Impacts (PEI) with 5.56 PEI/kg butanol. Both ABE fermentation routes obtained Fire and Explosion Damage Index (FEDI) values above 300 for acetone and ethanol recovery/purification stages. Both routes are classified as “hazardous” considering the flammability of handled substances, and their relative safety performance is remarkably similar. These results pave the way toward deploying both routes for adding value to the cassava residues in North Colombia by applying safe, efficient, and environmentally friendly transformation technologies.

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Effect of salts formed by neutralization for the enzymatic hydrolysis of cellulose and acetone–butanol–ethanol fermentation

Abstract: The salts formed by neutralization after sulfuric, acetic, and citric acid pretreatments affected enzymatic hydrolysis of lignocellulosic materials and acetone–butanol–ethanol (ABE) fermentation to various degrees.

Cited by 10 publications

References 30 publications

Second-Generation Biofuel Production from the Marine Filter Feeder Ciona intestinalis

Second-Generation Biofuel Production from the Marine Filter Feeder Ciona intestinalis

Cell Factories for Industrial Production Processes: Current Issues and Emerging Solutions

Technical, Environmental, and Process Safety Assessment of Acetone-Butanol-Ethanol Fermentation of Cassava Residues

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