Effect of salt stress on the activity of bromelain in pineapple plants grown In vitro

Vilanova-Neta, Jaci; Menezes, Diego Batista; Barreto, M. S.; Souza, Jaqueline Barroso de; Carvalho-Neto, Antônio; Lédo, A. da S.; Ruzene, Denise Santos; Silva, Daniel

doi:10.1186/1753-6561-8-s4-p199

Cited by 2 publications

(3 citation statements)

References 5 publications

(5 reference statements)

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“…A typical reason for ammonia-N accumulation was the proteolytic enzymes (Kung and Shaver, 2001 ). Even most plant proteolytic enzymes in alfalfa silage showed greater activities at pH 5.0–6.0 (Yang et al, 2019 ), but excess ions can inhibit proteolytic enzymes (Jaci et al, 2014 ), which might be an explanation for the reduction in ammonia-N formation in the MS and HS silage. After alfalfa fermentation, the content of SP increased from 66–108 to 124–150, which may be related to the hydrolysis of protein during the fermentation process.…”

Section: Discussionmentioning

confidence: 99%

The Potential Effects on Microbiota and Silage Fermentation of Alfalfa Under Salt Stress

Lü

Wang

et al. 2021

Front. Microbiol.

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This study investigated the fermentation quality of alfalfa grown in different salt stress regions in China. Following the production of silage from the natural fermentation of alfalfa, the interplay between the chemical composition, fermentation characteristics, and microbiome was examined to understand the influence of these factors on the fermentation quality of silage. The alfalfa was cultivated under salt stress with the following: (a) soil content of <1%0 (CK); (b) 1–2%0 (LS); (c) 2–3%0 (MS); (d) 3–4%0 (HS). The pH of the silage was high (4.9–5.3), and lactic acid content was high (26.3–51.0 g/kg DM). As the salt stress increases, the NA+ of the silages was higher (2.2–5.4 g/kg DM). The bacterial alpha diversities of the alfalfa silages were distinct. There was a predominance of desirable genera including Lactococcus and Lactobacillus in silage produced from alfalfa under salt stress, and this led to better fermentation quality. The chemical composition and fermentation characteristics of the silage were closely correlated with the composition of the bacterial community. Furthermore, NA+ was found to significantly influence the microbiome of the silage. The results confirmed that salt stress has a great impact on the quality and bacterial community of fresh alfalfa and silage. The salt stress and plant ions were thus most responsible for their different fermentation modes in alfalfa silage. The results of the study indicate that exogenous epiphytic microbiota of alfalfa under salt stress could be used as a potential bioresource to improve the fermentation quality.

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Section: Discussionmentioning

confidence: 99%

The Potential Effects on Microbiota and Silage Fermentation of Alfalfa Under Salt Stress

Lü

Wang

et al. 2021

Front. Microbiol.

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“…Compared with the raw material, the neutral detergent fiber (NDF), and acid detergent fiber (ADF) contents in silages of different saline-alkali soils decreased, thereby supplementing the fermentation substrates of LAB and reducing losses. The accumulation of ammonia nitrogen (NH 3 -N) is mainly driven by plant proteases and protein hydrolytic enzymes [11], and the Na + content has an impact on the activity of proteases [29]. When the Na + concentration is below 60 mg/L, Na + promotes the activity of proteases, while when the Na + concentration is above 60 mg/L, Na + inhibits the activity of proteases.…”

Section: Discussionmentioning

confidence: 99%

“…Ammonia nitrogen accumulation is typically caused by protein hydrolytic enzymes [30]. Even though most plant proteases in alfalfa silage show higher activity at pH 5.0-6.0 [31], excessive ions can inhibit the activity of proteases [29]. This may be the reason why Na + inhibits the activity of proteases during ensiling.…”

Section: Discussionmentioning

confidence: 99%

Comparative Na+ and K+ Profiling Reveals Microbial Community Assembly of Alfalfa Silage in Different Saline-Alkali Soils

Li,

Bao,

et al. 2023

Fermentation

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Alfalfa cultivated in saline-alkaline soils exhibits a high buffering capacity but low carbohydrate content, posing challenges in the production of high-quality silage feed. This study investigated alfalfa plants grown under varying conditions: mild (QE, salt content 1–2%), moderate (ZE, salt content 2–3%), severe (HE, salt content 3–4%), and non-saline-alkaline (CON, salt content < 1%). Employing a two-factor experimental design, we analyzed the fluctuations in nutritional quality, microbial abundance, and community composition of different salt–alkaline alfalfa materials and silage feeds. Furthermore, we elucidated the fermentation mechanism involved in salt–alkaline alfalfa ensiling. Following a 60-day ensiling period, the ZE and HE treatments led to substantial reductions in pH, acetic acids (AA), branched-chain butyric acids (BA) content, facultative anaerobic bacteria, and Escherichia coli populations (p < 0.05). Conversely, the ZE and HE treatments increased lactic acid (LA) content and the population of lactic acid bacteria (LAB) (p < 0.05). Additionally, these treatments significantly mitigated protein losses in both raw alfalfa and silage feeds (p < 0.05), while remarkably augmenting the water-soluble carbohydrates (WSC), Na+, and K+ content of alfalfa materials. Sodium ions were found to exert a considerable influence on bacterial community composition during salt–alkaline alfalfa ensiling, with Enterococcus, Lactococcus, and Lactobacillus identified as the predominant fermentative microorganisms. Overall, moderately salt-alkaline alfalfa materials displayed optimal nutritional and fermentation quality, ensuring favorable nutritional attributes and fermentation outcomes under such conditions.

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Effect of salt stress on the activity of bromelain in pineapple plants grown In vitro

Cited by 2 publications

References 5 publications

The Potential Effects on Microbiota and Silage Fermentation of Alfalfa Under Salt Stress

The Potential Effects on Microbiota and Silage Fermentation of Alfalfa Under Salt Stress

Comparative Na+ and K+ Profiling Reveals Microbial Community Assembly of Alfalfa Silage in Different Saline-Alkali Soils

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