Effect of salt stress on the genes expression of the vacuolar H+ -pyrophosphatase and Na+/H+  antiporter in Rubia tinctorum

Toranj, Sanaz; Aliabad, Kazem Kamali; Abbaspour, Hossein; Saeedpour, Ali

doi:10.1007/s11033-019-05124-8

Cited by 7 publications

(3 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The contribution of Na + to osmotic potential was up to 60% in halophyte Solanum chilense ( Gharbi et al, 2017 ), which is mainly achieved by sequestration of Na + into the vacuole. Tonoplast Na + /H + antiporters NHX and AVP mediated Na + movement in the vacuole ( Liu et al, 2012 ; Toranj et al, 2020 ), Meanwhile, halophytes secreted excess Na + by salt glands, and the rate of salt secretion increased with the increasing salinity ( Sanadhya et al, 2015 ). In K. caspia, KcSOS1 silenced-lines disrupted the Na + transport, then decreased Na + secretion rates ( Guo et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

ROS scavenging and ion homeostasis is required for the adaptation of halophyte Karelinia caspia to high salinity

et al. 2022

View full text Add to dashboard Cite

The halophyte Karelinia caspia has not only fodder and medical value but also can remediate saline-alkali soils. Our previous study showed that salt-secreting by salt glands is one of main adaptive strategies of K. caspia under high salinity. However, ROS scavenging, ion homeostasis, and photosynthetic characteristics responses to high salinity remain unclear in K. caspia. Here, physio-biochemical responses and gene expression associated with ROS scavenging and ions transport were tested in K. caspia subjected to 100–400 mM NaCl for 7 days. Results showed that both antioxidant enzymes (SOD, APX) activities and non-enzymatic antioxidants (chlorogenic acid, α-tocopherol, flavonoids, polyamines) contents were significantly enhanced, accompanied by up-regulating the related enzyme and non-enzymatic antioxidant synthesis gene (KcCu/Zn-SOD, KcAPX6, KcHCT, KcHPT1, Kcγ-TMT, KcF3H, KcSAMS and KcSMS) expression with increasing concentrations of NaCl. These responses are beneficial for removing excess ROS to maintain a stable level of H2O2 and O2− without lipid peroxidation in the K. caspia response to high salt. Meanwhile, up-regulating expression of KcSOS1/2/3, KcNHX1, and KcAVP was linked to Na+ compartmentalization into vacuoles or excretion through salt glands in K. caspia. Notably, salt can improve the function of PSII that facilitate net photosynthetic rates, which is helpful to growing normally in high saline. Overall, the findings suggested that ROS scavenging systems and Na+/K+ transport synergistically contributed to redox equilibrium, ion homeostasis, and the enhancement of PSII function, thereby conferring high salt tolerance.

show abstract

Section: Introductionmentioning

confidence: 99%

ROS scavenging and ion homeostasis is required for the adaptation of halophyte Karelinia caspia to high salinity

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Plants maintain ion homeostasis in response to stress environments by reducing excess Na + (reducing Na + uptake, promoting Na + efflux, and compartmentalizing Na + ) to enhance salt tolerance. Na + efflux and compartmentalization are active transport processes mainly driven by the proton gradient generated by H + -ATPase and H + -PPase, and mediated by Na + /H + antiporters [ 51 ]. Besides Na + , Cl − efflux and compartmentalization are also important salt tolerance mechanisms.…”

Section: Discussionmentioning

confidence: 99%

Ascorbic Acid Improves Tomato Salt Tolerance by Regulating Ion Homeostasis and Proline Synthesis

Chen,

Han,

Cong

et al. 2024

Plants

View full text Add to dashboard Cite

In this study, processing tomato (Solanum lycopersicum L.) ‘Ligeer 87-5’ was hydroponically cultivated under 100 mM NaCl to simulate salt stress. To investigate the impacts on ion homeostasis, osmotic regulation, and redox status in tomato seedlings, different endogenous levels of ascorbic acid (AsA) were established through the foliar application of 0.5 mM AsA (NA treatment), 0.25 mM lycorine (LYC, an inhibitor of AsA synthesis; NL treatment), and a combination of LYC and AsA (NLA treatment). The results demonstrated that exogenous AsA significantly increased the activities and gene expressions of key enzymes (L-galactono-1,4-lactone dehydrogenase (GalLDH) and L-galactose dehydrogenase (GalDH)) involved in AsA synthesis in tomato seedling leaves under NaCl stress and NL treatment, thereby increasing cellular AsA content to maintain its redox status in a reduced state. Additionally, exogenous AsA regulated multiple ion transporters via the SOS pathway and increased the selective absorption of K+, Ca2+, and Mg2+ in the aerial parts, reconstructing ion homeostasis in cells, thereby alleviating ion imbalance caused by salt stress. Exogenous AsA also increased proline dehydrogenase (ProDH) activity and gene expression, while inhibiting the activity and transcription levels of Δ1-pyrroline-5-carboxylate synthetase (P5CS) and ornithine-δ-aminotransferase (OAT), thereby reducing excessive proline content in the leaves and alleviating osmotic stress. LYC exacerbated ion imbalance and osmotic stress caused by salt stress, which could be significantly reversed by AsA application. Therefore, exogenous AsA application increased endogenous AsA levels, reestablished ion homeostasis, maintained osmotic balance, effectively alleviated the inhibitory effect of salt stress on tomato seedling growth, and enhanced their salt tolerance.

show abstract

“…b). However, some studies also showed that overexpression of VP is sufficient for plants survival under certain conditions such as drought, salt and heat stresses (Li et al , ; Segami et al , ; Toranj et al , ). Thus, we speculate that there is a certain threshold of VP activity under nonstressed conditions for cell viability and plant growth.…”

Section: Discussionmentioning

confidence: 99%

Chinese wheat mosaic virus‐derived vsiRNA‐20 can regulate virus infection in wheat through inhibition of vacuolar‐ (H⁺)‐PPase induced cell death

Yang¹,

Zhang²,

Li³

et al. 2020

New Phytologist

View full text Add to dashboard Cite

Summary Vacuolar (H+)‐PPases (VPs), are key regulators of active proton (H+) transport across membranes using the energy generated from PPi hydrolysis. The VPs also play vital roles in plant responses to various abiotic stresses. Their functions in plant responses to pathogen infections are unknown. Here, we show that TaVP, a VP of wheat (Triticum aestivum) is important for wheat resistance to Chinese wheat mosaic virus (CWMV) infection. Furthermore, overexpression of TaVP in plants induces the activity of PPi hydrolysis, leading to plants cell death. A virus‐derived small interfering RNA (vsiRNA‐20) generated from CWMV RNA1 can regulate the mRNA accumulation of TaVP in wheat. The accumulation of vsiRNA‐20 can suppress cell death induced by TaVP in a dosage‐dependent manner. Moreover, we show that the accumulation of vsiRNA‐20 can affect PPi hydrolysis and the concentration of H+ in CWMV‐infected wheat cells to create a more favorable cellular environment for CWMV replication. We propose that vsiRNA‐20 regulates TaVP expression to prevent cell death and to maintain a weak alkaline environment in cytoplasm to enhance CWMV infection in wheat. This finding may be used as a novel strategy to minimize virus pathogenicity and to develop new antiviral stratagems.

show abstract

Effect of salt stress on the genes expression of the vacuolar H+ -pyrophosphatase and Na+/H+ antiporter in Rubia tinctorum

Cited by 7 publications

References 29 publications

ROS scavenging and ion homeostasis is required for the adaptation of halophyte Karelinia caspia to high salinity

ROS scavenging and ion homeostasis is required for the adaptation of halophyte Karelinia caspia to high salinity

Ascorbic Acid Improves Tomato Salt Tolerance by Regulating Ion Homeostasis and Proline Synthesis

Chinese wheat mosaic virus‐derived vsiRNA‐20 can regulate virus infection in wheat through inhibition of vacuolar‐ (H⁺)‐PPase induced cell death

Contact Info

Product

Resources

About

Effect of salt stress on the genes expression of the vacuolar H+ -pyrophosphatase and Na+/H+ antiporter in Rubia tinctorum

Cited by 7 publications

References 29 publications

ROS scavenging and ion homeostasis is required for the adaptation of halophyte Karelinia caspia to high salinity

ROS scavenging and ion homeostasis is required for the adaptation of halophyte Karelinia caspia to high salinity

Ascorbic Acid Improves Tomato Salt Tolerance by Regulating Ion Homeostasis and Proline Synthesis

Chinese wheat mosaic virus‐derived vsiRNA‐20 can regulate virus infection in wheat through inhibition of vacuolar‐ (H+)‐PPase induced cell death

Contact Info

Product

Resources

About

Chinese wheat mosaic virus‐derived vsiRNA‐20 can regulate virus infection in wheat through inhibition of vacuolar‐ (H⁺)‐PPase induced cell death