Effect of salivary CYP4EM1 and CYP4EM2 gene silencing on the life span of Chagas disease vector <scp>Rhodnius prolixus</scp> (Hemiptera, Reduviidae) exposed to sublethal dose of deltamethrin

Paim, Rafaela M.M.; Pessoa, Grasielle Caldas D’Ávila; Nascimento, Bruno Warlley Leandro; Nascimento, Ana Mércia Dias; Pinheiro, Luiz C. S.; Koerich, Leonardo B.; Diotaiuti, Liléia; Araújo, Ricardo Nascimento; Sant’Anna, Maurício Roberto Viana; Gontijo, Nelder F.; Pereira, Marcos H.

doi:10.1111/imb.12737

Cited by 7 publications

(10 citation statements)

References 52 publications

(61 reference statements)

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“…In Rhodnius prolixus , RproCYP4M1 gene is highly expressed in salivary glands (Paim et al, 2021). RNAi knockdown of RproCYP4M1 increased the susceptibility and mortality of its nymphs subsequently exposed to deltamethrin (Paim et al, 2021). As a salivary protein, CYP6P9a is associated with feeding success and blood meal size of A. funestus (Nouage et al, 2020).…”

Section: Resultsmentioning

confidence: 99%

“…Salivary gland produces enzyme‐rich fluids acting in digestion (Cantón & Bonning, 2020). In Rhodnius prolixus , RproCYP4M1 gene is highly expressed in salivary glands (Paim et al, 2021). RNAi knockdown of RproCYP4M1 increased the susceptibility and mortality of its nymphs subsequently exposed to deltamethrin (Paim et al, 2021).…”

Section: Resultsmentioning

confidence: 99%

“…Salivary gland produces enzyme-rich fluids acting in digestion (Cantón & Bonning, 2020). In Rhodnius prolixus, RproCYP4M1 gene is highly expressed in salivary glands (Paim et al, 2021).…”

Section: Expression Pattern Analysis Of the T Molitor Cyp Genesmentioning

confidence: 99%

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Genome‐wide and expression‐profiling analyses of the cytochrome P450 genes in Tenebrionidea

Wang

et al. 2022

Arch Insect Biochem Physiol

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Cytochrome P450 monooxygenases (CYPs) are present in almost all areas of the tree of life. As one of the largest and most diverse superfamilies of multifunctional enzymes, they play important roles in the metabolism of xenobiotics and biosynthesis of endogenous compounds, shaping the success of insects. In this study, the CYPome (an omics term for all the CYP genes in a genome) diversification was examined in the four Tenebrionidea species through genome-wide analysis. A total of 483 CYP genes were identified, of which 103, 157, 122, and 101 were respectively deciphered from the genomes of Tebebrio molitor, Asbolus verucosus, Hycleus cichorii and Hycleus phaleratus. These CYPs were classified into four major clans (mitochondrial, CYP2, CYP3, and CYP4), and clans CYP3 and CYP4 are most diverse. Phylogenetic analysis showed that most CYPs of these Tenebrionidea beetles from each clan had a very close 1:1 orthology to each other, suggesting that they originate closely and have evolutionally conserved function. Expression analysis at different developmental stages and in various tissues showed the life stage-, gut-, salivary gland-, fat body-, Malpighian tubule-, antennae-, ovary-and testis-specific expression patterns of T. molitor CYP genes, implying their various potential roles in development, detoxification, immune response, digestion, olfaction, and reproduction.Our studies provide a platform to understand the evolution

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Genome‐wide and expression‐profiling analyses of the cytochrome P450 genes in Tenebrionidea

Wang

et al. 2022

Arch Insect Biochem Physiol

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“…Remarkably, the expression of CYP4EM1 was much higher in salivary gland than in any other organ/tissue, and it was modulated after insect blood meal. Knocking down of CYP4EM1 by RNAi induced deleterious effects in R. prolixus nymphs after topical application of deltamethrin, suggesting an important role in detoxification (29).…”

Section: Roles Of Triatomine Cyp4 Clan In Insecticide Detoxification ...mentioning

confidence: 99%

“…The role of three CYP4EM subfamily members (genes EM1, EM2 and EM3) was evaluated in vivo during R. prolixus blood meal and after deltamethrin exposure (29). A differential expression pattern of these genes was found in different insect organs and tissues; CYP4EM1 and CYP4EM2 were expressed in the integument, salivary glands, midgut, fat body and Malpighian tubules, whereas CYP4EM3 was expressed only in the integument, fat body and Malpighian tubules.…”

Section: Roles Of Triatomine Cyp4 Clan In Insecticide Detoxification ...mentioning

confidence: 99%

Cytochrome P450 Genes of the CYP4 Clan and Pyrethroid Resistance in Chagas Disease Vectors

2022

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Triatomine insects are vectors of the protozoan Trypanosoma cruzi, the causative agent of Chagas disease. Although residual pyrethroid spraying has been a successful vector control strategy for many years, a growing number of pyrethroid-resistance foci is being documented, mainly in Triatoma infestans, that led to failures in vector elimination. Insecticide resistance is a multifactorial phenomenon that often implies a combination of three different mechanisms: increased insecticide detoxification, reduced affinity of the site of action, and reduced insecticide penetration through the cuticle. All three mechanisms were reported in pyrethroid-resistant T. infestans. Cytochrome P450s are enzymes involved in the metabolism of xenobiotics and endogenous chemicals. They are encoded by CYP genes and classified into different families and clans. In triatomines, the CYP4 clan is divided in two families, CYP3093 and CYP4, and both exhibit genome-wide, triatomine-specific gene expansions. Some members from each family have been reported to be involved in two of the mechanisms mentioned above, i.e., they participate in insecticide detoxification in different organs and tissues, and in the synthesis of cuticular hydrocarbons, which ultimately can contribute to a reduced insecticide penetration. The aim of this manuscript is to review the current state of knowledge of P450 genes belonging to the CYP4 clan in triatomines and to highlight their potential role in insecticide resistance.

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Molecular identification of carboxylesterase genes and their potential roles in the insecticides susceptibility of Grapholita molesta

Jia

Zhang

et al. 2023

Insect Molecular Biology

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Grapholita molesta is one of the most damaging pests worldwide in stone and pome fruits. Application of chemical pesticides is still the main method to control this pest, which results in resistance to several types of insecticides. Carboxylesterase (CarE) is one of the important enzymes involved in the detoxification metabolism and tolerance of xenobiotics and insecticides. However, the roles of CarEs in insecticides susceptibility of G. molesta are still unclear. In the present study, the enzyme activity of CarEs and the mRNA expression of six CarE genes were consistently elevated after treatment with three insecticides (emamectin benzoate, lambda-cyhalothrin, and chlorantraniliprole).According to spatio-temporal expression profiles, six CarE genes expressed differently in different developmental stages, and highly expressed in some detoxification metabolic organs. RNAi-mediated knockdown of these six CarE genes indicated that the susceptibility of G. molesta to all these three insecticides were obviously raised after GmCarE9, GmCarE14, GmCarE16, and GmCarE22 knockdown, respectively. Overall, these results demonstrated that GmCarE9, GmCarE14, GmCarE16, and GmCarE22 play a role in the susceptibility of G. molesta to emamectin benzoate, lambda-cyhalothrin, and chlorantraniliprole treatment. This study expands our understanding of CarEs in insects, that the same CarE gene could participate in the susceptibility to different insecticides.

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Effect of salivary CYP4EM1 and CYP4EM2 gene silencing on the life span of Chagas disease vector Rhodnius prolixus (Hemiptera, Reduviidae) exposed to sublethal dose of deltamethrin

Cited by 7 publications

References 52 publications

Genome‐wide and expression‐profiling analyses of the cytochrome P450 genes in Tenebrionidea

Genome‐wide and expression‐profiling analyses of the cytochrome P450 genes in Tenebrionidea

Cytochrome P450 Genes of the CYP4 Clan and Pyrethroid Resistance in Chagas Disease Vectors

Molecular identification of carboxylesterase genes and their potential roles in the insecticides susceptibility of Grapholita molesta

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