Effect of saliva decontamination protocol on the adhesion to dentin with a universal adhesive

Abstract

To evaluate the influence and effectiveness of the salivary decontamination protocol after polymerization of a universal adhesive on dentin adhesive strength, after 24 hours and 6 months of ageing. Methods: Fifty intact molars were sectioned in order to obtain two slices of dentin from each tooth. In all specimens except the control group (C-no contamination), after applying and light-curing the adhesive system (Scotchbond Universal), the adhesion surface was contaminated with human saliva, and was then subjected to a decontamination method that differed between groups (W-decontamination with water; W+A-decontamination with water and reapplication of adhesive; E-decontamination with ethanol; E+A-decontamination with ethanol and reapplication of adhesive). Shear bond strength was tested at 24 hours and 6 months of aging, until fracture, and the failure mode was observed. Data were statistically analyzed using non-parametric Mann-Whitney and Kruskal-Wallis tests (α=0.05). Results: At 24 hours of aging, the decontaminant (p=0.289) and the reapplication of the adhesive (p=0.072) did not influence the adhesive strength values, and all contaminated groups obtained significantly (p<0.05) lower adhesive strength values than the control group. At 6 months, the reapplication of the adhesive (W+A and E+A) provided increased adhesion values (p=0.001), but no differences were observed between the decontaminants (p=0.314). Only the W+A group yielded a statistically (p=0.376) similar value to the control group. Conclusion: When there is salivary contamination of the adhesion area after polymerization of the universal adhesive tested, water decontamination should be performed followed by a

Effect of contamination and decontamination methods on the bond strength of adhesive systems to dentin: A systematic review

“…37 Also, when salivary contamination occurred after polymerizing the adhesive, water decontamination should be accomplished followed by an adhesive reapplication. 42 All in all, rinsing, drying, rebonding; rinsing, air-drying, rebonding; etching, rinsing, blot-drying, rebonding; or etching, rinsing, drying could be sufficient for decreasing the adverse effect of saliva. 39 Besides saliva contamination, some studies investigated the effect of blood contaminant on the bond strength of adhesive systems to dentin.…”

Comparative evaluation of different surface treatments of the salivary contaminated dentin on the shear bond strength of self-etch adhesives: An in vitro study

“…Various studies done to date have reported variable results. Even few studies[ 4 5 ] have reported that the saliva contamination of dentin had no adverse effect on the bonding efficiency of one-bottle adhesive systems while others have shown that the saliva contamination of the dentin surface produced a significant decrease in the bond strength[ 6 7 8 9 10 11 ]…”

“…The results are in accordance with many other studies that showed that contamination of the bonded surface after curing of the self-etch adhesive results in lower bond strength. [ 6 7 9 10 11 13 ]…”

“…The contaminated layer acts as a semipermeable membrane after polymerization which causes decrease in the bond strength. [ 9 ]…”

Microhardness and flexural strength of two 3D-printed denture base resins

“…A power analysis was performed based on a pilot study to estimate the sample size of both tests (n=8) and provide statistical significance (α=0.05) at 80% power and an effect size of 0.25. 13,30 Four denture base resins were selected: V-Print Dentbase (VOCO GmbH, Germany) and Denture 3D+ (NextDent BV, The Netherlands) as light-cured denture base resins suitable for 3D printing and Probase Hot (Ivoclar Vivadent AG, Liechtenstein) and Villacryl Rapid (Zhermack SpA, Italy) as heat-cured denture base resins for conventional production (Table 1). Eight parallelepiped-shaped (64x10x3.3 mm) specimens were prepared from each resin according to the respective manufacturer's instructions and considering specific standards.…”