Effect of salinity and abscisic acid on accumulation of glycinebetaine and betaine aldehyde dehydrogenase mRNA in Sorghum leaves (Sorghum bicolor)

Saneoka, Hirofumi; Ishiguro, Shigeo; Moghaieb, Reda E. A.

doi:10.1078/0176-1617-00058

Cited by 41 publications

(25 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…: Pillai and Yanagihara 2001;Saneoka and Ishiguro 2001;Yokoi et al 2002;Yu and Zhou 2003;Fukuda and Chiba 2004), but most of these studies have been conducted using a high concentration of NaCl alone, a salt shock, which causes changes in transcription of genes responsive to the osmotic effect of NaCl and not its ionic component. In the present study, the cDNA-AFLP technique has been used to identify some genes of IR4630 (a salt tolerant line) that were induced by the ionic effect of salt, and not expressed in IR15324 (a sensitive line): that is AFLP bands that appeared in the shoots of the tolerant line and not the sensitive line and in salttreated plants but not in control plants or those treated with mannitol.…”

Section: Introductionmentioning

confidence: 99%

The ionic effects of NaCl on physiology and gene expression in rice genotypes differing in salt tolerance

Roshandel

Flowers

2008

Plant Soil

View full text Add to dashboard Cite

The effects of ionic stress on the physiology and gene expression of two rice genotypes (IR4630 and IR15324) that differ in salt tolerance, were investigated by evaluating changes in the biomass, Na + and K + concentrations and applying the cDNA-AFLP technique to highlight changes in gene expression. Over 8 days of salinisation, the effect of NaCl on the reduction of biomass (dry weight) was apparent from 24 h after salinisation (the first time point), indicating that the consequences of the build up of Na + (and Cl -) in the leaves of both lines was rapid. Furthermore, root growth of IR15324 was much more sensitive to salt than that of IR4630 (the reduction in root dry weight compared to nonsalinised plants was three times greater in IR15324 than IR4630). The two rice lines also differed in their Na + accumulation in saline conditions, a difference that was more marked in the shoots, particularly at the final harvest, than in the roots. Under salt stress, the K + content (µmol/shoot) increased over four successive harvests (24, 48, 96, 192 h) in both lines, but was always greater in IR4630 than in IR15324: differences in Na + /K + ratio appear to be an important determinant of salt tolerance in rice. To separate osmotic from ionic effects of salt, mannitol was applied as a non-ionic osmoticum at an osmotic potential estimated to be equivalent to 50 mM NaCl. Messenger RNA was sampled at 0.5, 6, 24, 48 and 192 hours after salinisation. Several products (AFLPbands) were detected, which were upregulated in the response to ionic effects of salt in the tolerant line (IR4630) and not expressed in the sensitive line (IR15324). Bioinformatic analysis indicated three of these AFLP-bands have a high-degree of sequence similarity with the genes encoding a proline rich protein, senescence associated protein and heat-shock protein. The data are novel in that they differentially highlight changes induced by the ionic rather than osmotic effects of salt and in a tolerant rather than a sensitive genotype. The possible roles of the products of these genes are discussed.

show abstract

Section: Introductionmentioning

confidence: 99%

The ionic effects of NaCl on physiology and gene expression in rice genotypes differing in salt tolerance

Roshandel

Flowers

2008

Plant Soil

View full text Add to dashboard Cite

show abstract

“…In recent years, the relationships between ABA and betaine biosynthesis in plants under environmental stresses have attracted much attention. Some reports suggested that ABA is involved in the betaine biosynthesis in plants under environmental stresses (Ishitani et al 1995, Abernethy and McManus 1998, Nakamura et al 2001, Saneoka et al 2001. Other reports, however, indicated that ABA may not necessarily be associ-ated with betaine accumulation in sugar beet (McCue and Hanson 1992b), oat (Maldonado et al 1997), and periwinkle (Rai et al 2001) exposed to stress.…”

Section: Introductionmentioning

confidence: 99%

“…Pear, unlike jujube and grapevine, is a betaine accumulator under drought stress (Gao et al 2002, Gao et al 2004). Like most stress-induced betaine accumulators in plants (McCue and Hanson 1992b, Rhodes and Hanson 1993, Kishitani et al 1994, Maldonado et al 1997, Abernethy and McManus 1998, Saneoka et al 2001, Xing and Rajashekar 2001, the pear plants had 2-fold or even higher betaine concentrations in their leaves under drought stress (Gao et al 2002, Gao et al 2004). Exogenously applied betaine improved the tolerance of the pear to drought (Gao et al 2004).…”

Section: Introductionmentioning

confidence: 99%

“…Higher plants, as other organisms, accumulate osmoprotectants in the cytoplasmic compartment of the cells under stressful conditions, such as high salt (Arakawa et al 1992, Rhodes and Hanson 1993, Saneoka et al 2001, drought (Holmstrom et al 1996, Abernethy and McManus 1998, Xing and Rajashekar 2001, low temperature (Koster and Lynch 1992, Kishitani et al 1994, Rajashekar et al 1999, and high temperature (Murata et al 1992). Among the osmoprotectants are polyols and their derivatives, amino acids and quaternary ammonium compounds.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Abscisic Acid is Involved in the Water Stress-Induced Betaine Accumulation in Pear Leaves

Gao

Pan

et al. 2004

Plant and Cell Physiology

View full text Add to dashboard Cite

; ABA exogenously applied to the leaves of the whole plants of pear (Pyrus bretschneideri Redh. cv. Suly grafted on Pyrus betulaefolia Rehd.) significantly increased the betaine concentrations in the leaves when the plants were well watered. The plants subjected to 'drought plus ABA' treatment had significantly higher betaine concentrations in their leaves than those given drought treatment alone. The 'drought plus ABA' treatment increased the amount of betaine aldehyde dehydrogenase (BADH, EC 1.2.1.8) and its activity in the leaves more than did the drought treatment alone. The experiments with detached leaves showed that ABA treatment significantly increased the concentration of betaine, activity of BADH and apparent amount of BADH in non-dehydrated leaves, and enhanced the accumulation of betaine, activity of BADH and apparent amount of BADH in dehydrated leaves. These effects of ABA were both time-and dose-dependent. Two ABA isomers, (-)-cis, trans-ABA and 2-trans, 4-trans-ABA, had no effect on the betaine accumulation in the leaves, showing that the ABA-induced effects are specific. These data demonstrate that ABA is involved in the drought-induced betaine accumulation in the pear leaves.

show abstract

“…A low Ca 2+ concentration under saline conditions may severely affect the functions of membranes as barriers to ion loss from cells (Boursier and Läuchli, 1990). Various organic and inorganic solutes such as K + , Na + , Cl -, proline, and glycinebetaine have been reported to contribute to such osmotic adjustment (Saneoka et al, 2001). Salinity inhibits the accumulation of K + and Ca 2+ in roots and stems.…”

Section: Dry Weight (Mg)mentioning

confidence: 99%

Sweet Sorghum: Salt Tolerance and High Biomass Sugar Crop

Almodares¹,

Hadi²,

Kharazian³

2011

Biomass - Detection, Production and Usage

View full text Add to dashboard Cite

Effect of salinity and abscisic acid on accumulation of glycinebetaine and betaine aldehyde dehydrogenase mRNA in Sorghum leaves (Sorghum bicolor)

Cited by 41 publications

References 40 publications

The ionic effects of NaCl on physiology and gene expression in rice genotypes differing in salt tolerance

The ionic effects of NaCl on physiology and gene expression in rice genotypes differing in salt tolerance

Abscisic Acid is Involved in the Water Stress-Induced Betaine Accumulation in Pear Leaves

Sweet Sorghum: Salt Tolerance and High Biomass Sugar Crop

Contact Info

Product

Resources

About