Effect of Sairei-to on gentamicin nephrotoxicity in rats

Abstract

The effect of Sairei-to, an oriental traditional medicine, against gentamicin nephrotoxicity was examined in gentamicin nephrotoxicity rat models. Gentamicin nephrotoxicity was induced by s.c. injection of gentamicin (100 mg/kg/day, for 3 days) in male Sprague-Dawley rats. Renal functions of two rat groups were compared, one eating rat chow containing 2.5% Sairei-to, the other eating normal rat chow. Sairei-to administration reduced the increase of urinary N-acetyl-beta-D-glucosaminidase (NAG) and protein excretion, and decreased creatinine clearance induced by gentamicin. Gentamicin increased renal cortical malondialdehyde (MDA) concentration in normal diet group but not in the Sairei-to diet group. The renal cortical gentamicin concentration was not different between the two groups. In conclusion, Sairei-to shows reno-protective action against gentamicin nephrotoxicity, possibly through its anti-oxidant action.

Theoretical Basis for Herbal Medicines, Tokishakuyaku‐San and Sairei‐To, in the Treatment of Recurrent Abortion: Enhancing the Production of Granulocyte–Macrophage Colony‐Stimulating Factor in Decidual Stromal Cells

“…According to a previous report, an anti‐oxidant, pyrrolidine dithiocarbamate, preventing the degradation of mRNA in cultured pulmonary epithelial cells, increased GM‐CSF expression 23 . It has been demonstrated that the pharmacological effects of Toki and Sai are partly based on their anti‐oxidant action 24,25 . Therefore, the promotion of GM‐CSF expression observed in the present study might be related to anti‐oxidant pathway.…”

An Appraisal of Nephroprotection and the Scope of Natural Products in Combating Renal Disorders

“…The Japanese traditional remedy Sairei-to, a 12 herb mixture, has shown in human and animal studies to protect the kidney in gentamicin renal toxicity, IgA nephropathy, and lupus nephritis [62]. Another study in rats showed that extracts from the root of the plant Salvia miltiorriza (Danshen) along with fructose 1,6-diphosphate prevented the decline of renal cortical Na-K-ATPase activity induced by ischemia and gentamicin [63].…”

Medicinal herbal extracts – renal friend or foe? Part two: Herbal extracts with potential renal benefits

“…20 These benefits were hypothesized to have been caused by the anti-oxidant actions of ST, 20 given that gentamicin has been shown to exert renal tubular toxicity via enhanced generation of superoxide anion and hydroxyl radical in renal cortical mitochondria. 21 Saireito has similarly proved to be beneficial in a subtotal nephrectomy model of CRF, whereby animals administered 2.5% ST in their food had lower blood pressure, reduced renal damage, decreased protein excretion and greater efferent arteriolar dilation when compared to the untreated rats.…”