Effect of saikosaponin, a triterpene saponin, on apoptosis in lymphocytes: association with c‐<i>myc, p53</i>, and <i>bcl‐2</i> mRNA

Hsu, Ming Jen; Cheng, Jur-Shan; Huang, Hung‐Tse

doi:10.1038/sj.bjp.0703559

Cited by 48 publications

(23 citation statements)

References 40 publications

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“…Saikosaponins (A, B and D), the major components of Bupleuri Radix, have attracted a great deal of attention as anticancer agents [34][35][36][37]. Wu et al [34] reported that the increase of p-15…”

Section: Discussionmentioning

confidence: 99%

Effect of Bupleuri Radix Extracts on the Toxicity of 5‐Fluorouracil in HepG2 Hepatoma Cells and Normal Human Lymphocytes

Kang

Lee

Kim

et al. 2008

Basic Clin Pharma Tox

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Section: Discussionmentioning

confidence: 99%

Effect of Bupleuri Radix Extracts on the Toxicity of 5‐Fluorouracil in HepG2 Hepatoma Cells and Normal Human Lymphocytes

Kang

Lee

Kim

et al. 2008

Basic Clin Pharma Tox

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“…Triterpenoids exert various pharmacological effects to control many diseases, including cancer [3,6,[33][34][35][36]. The present study is the first to evaluate the potential anti-tumoural effects of maslinic acid, a new triterpenoid isolated as a majority compound from olive-pelt pomace.…”

Section: Discussionmentioning

confidence: 99%

(2α,3β)‐2,3‐Dihydroxyolean‐12‐en‐28‐oic acid, a new natural triterpene from Olea europea, induces caspase dependent apoptosis selectively in colon adenocarcinoma cells

et al. 2006

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Triterpenoids are known to induce apoptosis and to be anti-tumoural. Maslinic acid, a pentacyclic triterpene, is present in high concentrations in olive pomace. This study examines the response of HT29 and Caco-2 colon-cancer cell lines to maslinicacid treatment. At concentrations inhibiting cell growth by 50-80% (IC50HT29 = 61 ± 1 lM, IC80HT29 = 76 ± 1 lM and IC50Caco-2 = 85 ± 5 lM, IC80Caco-2 = 116 ± 5 lM), maslinic acid induced strong G0/G1 cell-cycle arrest and DNA fragmentation, and increased caspase-3 activity. However, maslinic acid did not alter the cell cycle or induce apoptosis in the nontumoural intestine cell lines IEC-6 and IEC-18. Moreover, maslinic acid induced cell differentiation in colon adenocarcinoma cells. These findings support a role for maslinic acid as a tumour suppressant and as a possible new therapeutic tool for aberrant cell proliferation in the colon. In this report, we demonstrate for the first time that, in tumoural cancer cells, maslinic acid exerts a significant anti-proliferation effect by inducing an apoptotic process characterized by caspase-3 activation by a p53-independent mechanism, which occurs via mitochondrial disturbances and cytochrome c release.

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“…So, high cytotoxic potential may be attributed to corchorusins which are abundant in seeds of Corchorus sp. (Khan et al, 2006), having structural similarity with saikosaponins (Hsu et al, 2000;Mahato & Pal, 1987). Saikosaponins and saikosaponin-like compounds have been reported to possess potent antitumor activity (Bachran et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Corchorus olitorius(jute) extract induced cytotoxicity and genotoxicity on human multiple myeloma cells (ARH-77)

İşeri

Yurtçu²,

Şahin

et al. 2013

Pharmaceutical Biology

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Context: Corchorus olitorius L. (Malvaceae) has industrial importance in world jute production and is a widely cultivated and consumed crop in Cyprus and in some Arabic countries. Objective: The present study investigated cytotoxic and genotoxic effects of leaf extracts (LE) and seed extracts (SE) of the C. olitorius on the multiple myeloma-derived ARH-77 cells. The extracts were also evaluated for their total phenol content (TPC) and free radical scavenging activity (FRSA). Materials and methods: C. olitorius was collected from Nicosia, Cyprus. TPC and FRSA were measured by Folin-Ciocalteu and DPPH free radical methods, respectively. Cytotoxicity was evaluated by the MTT assay (4-2048 mg/mL range), and DNA damage (at IC 50 and ½IC 50 ) was measured by the comet assay. Results and discussion: The LE had significantly higher total phenol (78 mg GAE/g extract) than the SE (2 mg GAE/g extract) with significantly higher FRSA (IC 50 LE: 23 mg/mL and IC 50 SE: 10 401 mg/mL). Both LE and SE exerted cytotoxic effects on cells after 48 h. The IC 50 of SE (17 mg/mL) was lower than LE (151 mg/mL), which demonstrates its higher cytotoxicity on cells. The extracts were applied at 150 and 75 mg/mL for LE and at 17 and 8.5 mg/mL for SE, and the results of the comet assay revealed that the extracts induced genotoxic damage on ARH-77 cells. In both 48 h leaf and seed extract treatments, genotoxic damage significantly increased with increasing concentrations at relevant cytotoxic concentrations. Conclusion: To our knowledge, this is the first report demonstrating the high cytotoxic potential of C. olitorius SE and the genotoxic potential of LE and SE.

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Effect of saikosaponin, a triterpene saponin, on apoptosis in lymphocytes: association with c‐myc, p53, and bcl‐2 mRNA

Cited by 48 publications

References 40 publications

Effect of Bupleuri Radix Extracts on the Toxicity of 5‐Fluorouracil in HepG2 Hepatoma Cells and Normal Human Lymphocytes

Effect of Bupleuri Radix Extracts on the Toxicity of 5‐Fluorouracil in HepG2 Hepatoma Cells and Normal Human Lymphocytes

(2α,3β)‐2,3‐Dihydroxyolean‐12‐en‐28‐oic acid, a new natural triterpene from Olea europea, induces caspase dependent apoptosis selectively in colon adenocarcinoma cells

Corchorus olitorius(jute) extract induced cytotoxicity and genotoxicity on human multiple myeloma cells (ARH-77)

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