Effect of RSR13, an allosteric hemoglobin modifier, on oxygenation in murine tumors: an in vivo electron paramagnetic resonance oximetry and bold MRI study

Hou, Huagang; Khan, Nadeem; O’Hara, Julia A.; Grinberg, Oleg Y.; Dunn, Jeff F.; Abajian, Michelle; Wilmot, Carmen M.; Makki, Malek; Demidenko, Eugene; Lu, Shiyi; Steffen, Robert P.; Swartz, Harold M.

doi:10.1016/j.ijrobp.2004.02.039

Cited by 33 publications

(45 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[60][61][62][63][64][65][66][67] The pO 2 in tumors will change during the course of therapy in response to the radiation and from changes in the relationship between oxygen consumption and the vascular supply to the tumor. The therapeutic ratio can be enhanced by using EPR oximetry to monitor tumor oxygen and using that information to determine the times when the pO 2 in the tumors is higher so that radiation should be administered.…”

Section: Enhancing Tumor Therapymentioning

confidence: 99%

Clinical applications of EPR: overview and perspectives

et al. 2004

Self Cite

View full text Add to dashboard Cite

The development and use of in vivo techniques for strictly experimental applications in animals has been very successful, and these results now have made possible some very attractive potential clinical applications. The area with the most obvious immediate, effective and widespread clinical use is oximetry, where EPR almost uniquely can make repeated and accurate measurements of pO 2 in tissues. Such measurements can provide clinicians with information that can impact directly on diagnosis and therapy, especially for oncology, peripheral vascular disease and wound healing. The other area of immediate and timely importance is the unique ability of in vivo EPR to measure clinically significant exposures to ionizing radiation 'after-the-fact', such as may occur due to accidents, terrorism or nuclear war. There are a number of other capabilities of in vivo EPR that also potentially could become extensively used in human subjects. In pharmacology the unique capabilities of in vivo EPR to detect and characterize free radicals could be applied to measure free radical intermediates from drugs and oxidative process. A closely related area of potential widespread applications is the use of EPR to measure nitric oxide. These often unique capabilities, combined with the sensitivity of EPR spectra to the immediate environment (e.g. pH, molecular motion, charge) have already resulted in some very productive applications in animals and these are likely to expand substantially in the near future. They should provide a continually developing base for extending clinical uses of in vivo EPR. The challenges for achieving full implementation include adapting the spectrometer for safe and comfortable measurements in human subjects, achieving sufficient sensitivity for measurements at the sites of the pathophysiological processes that are being measured, and establishing a consensus on the clinical value of the measurements.

show abstract

Section: Enhancing Tumor Therapymentioning

confidence: 99%

Clinical applications of EPR: overview and perspectives

et al. 2004

Self Cite

View full text Add to dashboard Cite

show abstract

“…Using an appropriate calibration curve, the line width of the EPR signal provides a sensitive measurement of tissue oxygen. This technique has been used to study the tissue pO 2 pO 2 in a wide range of experimental systems, including muscle (18,23), heart (11,16,17), brain (26,27,28,41,62), kidney (33,34), liver (35,44,64), skin (1) and tumors (29,30,47,49,50).…”

Section: Introduction To Epr Oximetrymentioning

confidence: 99%

Repetitive Tissue pO₂ Measurements by Electron Paramagnetic Resonance Oximetry: Current Status and Future Potential for Experimental and Clinical Studies

Khan

Williams²,

Hou³

et al. 2007

Antioxidants & Redox Signaling

Self Cite

118

133

View full text Add to dashboard Cite

Tissue oxygen plays a crucial role in maintaining tissue viability and in various diseases, including responses to therapy. Useful knowledge has been gained by methods that can give limited snapshots of tissue oxygen (e.g., oxygen electrodes) or evidence of a history of tissue hypoxia (e.g., EF5) or even indirect evidence by monitoring oxygen availability in the circulatory system (e.g., NMR methods). Each of these methods has advantages and significant limitations. EPR oximetry is a technique for direct measurement of tissue pO2, which has several advantages over the other existing methods for applications in which the parameter of interest is the pO2 of tissues, and information is needed over a time course of minutes to hours, and/or for repetitive measurements over days or weeks or years. The aim of this article is to provide an overview of EPR oximetry using particulates to readers who are not familiar with this technique and its potential in vivo and clinical applications. The data presented here are from the experiments currently being carried out in our laboratory. We are confident that in vivo EPR oximetry will play a crucial role in the understanding and clinical management of various pathologies in the years to come.

show abstract

“…There are several studies demonstrating the usefulness of EPR oximetry in tumor and liver biology [77][78][79][80][81]. As demonstrated in Study II, intrarenal tissue pO2 can be monitored for a prolonged time and EPR oximetry clearly has the potential to be implemented into clinic practice to monitor e.g.…”

Section: Discussionmentioning

confidence: 99%

The role of hypoxia for the development of diabetic nephropathy : Temporal relationship and involvement of endothelin receptor signaling

Franzén¹

2016

View full text Add to dashboard Cite

PrefaceThis thesis is the reflection of me, my work and my experiences. It has mostly been frustration but apparently I managed to stay alive the whole way through.For some it might have seemed easy but I am convinced that a big part has been extreme luck. I don't consider it my skill that it has sometimes run smoothly, because mostly it has been Murphy all over the place. Research is very much about luck. The luck to get the "right" animal batch, the luck to have equipment that isn't broken, the luck to have reviewers who like your paper, the luck that no one interferes with your settings, the luck to actually get results, etc etc. I could go one forever… Anyway, here it is. My work. To be understood or not. AbstractDiabetic nephropathy is one of the most common causes of end stage renal disease and develops in approximately one third of all diabetes patients. Disease progression is characterized by deteriorating glomerular filtration rate and escalating urinary albumin/protein excretion; both are used as clinical markers for disease progression. Recently, it has been proposed that intrarenal hypoxia is a unifying mechanism for chronic kidney disease, including diabetic nephropathy. Several mechanistic pathways have been linked to the development of intrarenal hypoxia and diabetic nephropathy including increased angiotensin II signaling, oxidative stress and hyperglycemia per se. Furthermore, pathological endothelin signaling has recently immerged as a possible contributing factor for chronic kidney disease and diabetic nephropathy. The overall aims of this thesis were therefore to determine the temporal relationship between development of intrarenal hypoxia and kidney disease as well as elucidate the potential link between endothelin signaling, intrarenal hypoxia and kidney disease in experimental insulinopenic diabetes. It is well established that different mouse strains have different susceptibility for kidney and cardiovascular disease. The first step was therefore to compare four commonly used mouse strains with regards to development of kidney disease after onset of insulinopenic diabetes. From the results of this study, we concluded that the NMRI mouse strain has a disease progression closest to the human disease and this strain was chosen in the subsequent studies in mice. The next step was to adapt and optimize a suitable method for repetitive measurements of intrarenal oxygen tension during the course of disease development. Electron paramagnetic resonance (EPR) oximetry had previously been used in tumor biology and was now adapted and optimized for measurements of kidney oxygenation in our diabetic mouse model. EPR oximetry in normoglycemic control mice recorded cortical oxygen tension values similar to previous reports using invasive techniques. Surprisingly, intrarenal hypoxia developed already within the first 72h after induction of hyperglycemia and persisted throughout the two-week study period. Importantly, this was well before albuminuria developed. The final part of this thesis was to invest...

show abstract

Effect of RSR13, an allosteric hemoglobin modifier, on oxygenation in murine tumors: an in vivo electron paramagnetic resonance oximetry and bold MRI study

Cited by 33 publications

References 48 publications

Clinical applications of EPR: overview and perspectives

Clinical applications of EPR: overview and perspectives

Repetitive Tissue pO₂ Measurements by Electron Paramagnetic Resonance Oximetry: Current Status and Future Potential for Experimental and Clinical Studies

The role of hypoxia for the development of diabetic nephropathy : Temporal relationship and involvement of endothelin receptor signaling

Contact Info

Product

Resources

About

Effect of RSR13, an allosteric hemoglobin modifier, on oxygenation in murine tumors: an in vivo electron paramagnetic resonance oximetry and bold MRI study

Cited by 33 publications

References 48 publications

Clinical applications of EPR: overview and perspectives

Clinical applications of EPR: overview and perspectives

Repetitive Tissue pO2 Measurements by Electron Paramagnetic Resonance Oximetry: Current Status and Future Potential for Experimental and Clinical Studies

The role of hypoxia for the development of diabetic nephropathy : Temporal relationship and involvement of endothelin receptor signaling

Contact Info

Product

Resources

About

Repetitive Tissue pO₂ Measurements by Electron Paramagnetic Resonance Oximetry: Current Status and Future Potential for Experimental and Clinical Studies