Effect of Royal Jelly on new bone formation in rapid maxillary expansion in rats

Abstract

BackgroundThe aim of this study was to evaluate the effects of long and short term systemic usage of royal jelly on bone formation in the expanded maxillary suture in a rat model.Material and MethodsTwenty eight Wistar albino rats were randomly divided into 4 equal groups: Control (C); Only Expansion (OE), Royal Jelly (RJ) group, Royal Jelly was given to rats by oral gavage only during the expansion and retention period; Royal Jelly plus Nursery (RJN) group, Royal Jelly was given to rats by oral gavage during their nursery phase of 40 days and during the retention period. After the 5 day expansion period was completed, the rats underwent 12 days of mechanical retention. All rats were sacrificed in same time. Histological examination was performed to determine the number of osteoclasts, number of osteoblasts, number of capillaries, inflammatory cell infiltration, and new bone formation.ResultsNew bone formation, number of osteoclasts, number of osteoblasts, and the number of capillaries in the expanded maxillary sutures were higher in the RJ and RJN groups than in the other groups. Statistical analysis also demonstrated that new bone formation and the number of osteoblasts was also highest in the RJN group.ConclusionsThe systemic administration of Royal Jelly in conjunction with rapid maxillary expansion may increase the quality of regenerated bone. Key words:Bone formation, rapid maxillary expansion, Royal jelly.

Does Shilajit Have an Effect on New Bone Remodelling in the Rapid Maxillary Expansion Treatment? A Biochemical, Histopathological and Immunohistochemical Study

“…The amount of new bone formation and capillary intensity were increased in the shilajit group relative to the OE group. These results are in agreement with another researchs that used antioxidants and found that increase in bone formation (1,14,15,25). In contrast to previous studies (14,15), there was no significant difference for the inflammatory cell infiltration between the groups in our study.…”

“…We preferred rat model for experimental maxillary expansion in this study similar to previous studies (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15). Altan et al (14) reported that rats and rabbits were well-established animal models for showing the changes in the midpalatal suture under forces.…”

“…In active stage, midpalatal suture broadens by expansion forces to disarticulate the two parts of the maxillary bone. In passive stage, suture tissues undergo bone remodeling, resorption and fiber rearrangement up to a new stability is provided (1,2). The expanded maxillary dental arch tends to return to its pretreatment widths after retention period.…”

Effects of the different administration frequencies of teriparatide (PTH [1‐34]) on new bone formation of expanded midpalatal sutures in rats: A histomorphometric and micro‐computed tomography analysis

“…In studies, the authors preferred a 5-day expansion period and 12-day retention period. 5,6,17,18 In the present study, the force (50 g) applied to separate the midpalatal suture and expansion/retention times was compatible with the literature.…”

“…Deynek and Ramoğlu 19 reported that the preferred amount of force for the purpose of separating the midpalatal suture in rats is between 50 and 70 grams. In studies, the authors preferred a 5‐day expansion period and 12‐day retention period 5,6,17,18 …”