Effect of Roux-en-Y gastric bypass-induced weight loss on the transcriptomic profiling of subcutaneous adipose tissue

González-Plaza, Juan José; Gutiérrez-Repiso, Carolina; García‐Serrano, Sara; Rodríguez‐Pacheco, Francisca; Garrido‐Sánchez, Lourdes; Santiago‐Fernández, Concepción; García-Arnés, Juan Antonio; Moreno-Ruiz, Francisco J.; Rodríguez‐Cañete, Alberto; Garcı́a-Fuentes, Eduardo

doi:10.1016/j.soard.2015.07.007

Cited by 23 publications

(13 citation statements)

References 30 publications

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“…That has the effect of more available energy for the immune system (synthesis of glucose, increased circulating levels of glucose) for fuelling the immune system, in keeping with the reported observations of the Warburg effect in some immune cell lines. Interestingly enough, this subchronic inflammation state is similar to what has been reported in obesity studies (72,73), and as reviewed here has a direct impact on the phenotype of NK cells.…”

Section: Crosstalk Between Metabolism and The Innate Immune Systemsupporting

confidence: 86%

Role of metabolism during viral infections, and crosstalk with the innate immune system

Plaza

Hulak

Каусова

et al. 2016

IRDR

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Section: Crosstalk Between Metabolism and The Innate Immune Systemsupporting

confidence: 86%

Role of metabolism during viral infections, and crosstalk with the innate immune system

Plaza

Hulak

Каусова

et al. 2016

IRDR

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“…The increase in the expression of several elongase genes in adipose tissue is consistent with earlier reports demonstrating that weight loss, induced by gastric bypass, leads to altered expression and methylation of genes in adipose tissue. 35 , 36 More specifically, we observed increases in the expressions of ELOVL3, ELOVL5 and ELOVL6. ELOVL5 plays a role in the elongation of n-3 and n-6 FAs, 37 and although the proportion of total n-6 FAs was not altered, the proportion of a long-chain adrenic acid (22:4 n-6) was significantly increased in adipose tissue, which could be a result of upregulation of ELOVL5 .…”

Section: Discussionmentioning

confidence: 56%

Alterations in fatty acid metabolism in response to obesity surgery combined with dietary counseling

et al. 2017

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Background:The effects of obesity surgery on serum and adipose tissue fatty acid (FA) profile and FA metabolism may modify the risk of obesity-related diseases.Methods:We measured serum (n=122) and adipose tissue (n=24) FA composition and adipose tissue mRNA expression of genes regulating FA metabolism (n=100) in participants of the Kuopio Obesity Surgery Study (KOBS, age 47.2±8.7 years, BMI 44.6±6.0, 40 men, 82 women) before and one year after obesity surgery. As part of the surgery protocol, all the subjects were instructed to add sources of unsaturated fatty acids, such as rapeseed oil and fatty fish, into their diet. The results were compared with changes in serum FA composition in 122 subjects from the Finnish Diabetes Prevention study (DPS) (age 54.3±7.1 years, BMI 32.2±4.6, 28 men, 94 women).Results:The proportion of saturated FAs decreased and the proportion of n-3 and n-6 FAs increased in serum triglycerides after obesity surgery (all P<0.002). Weight loss predicted changes in quantitative amounts of saturated FAs, monounsaturated FAs, n-3 and n-6 FAs in triglycerides (P<0.002 for all). Moreover, the changes in adipose tissue FAs reflected the changes in serum FAs, and some of the changes were associated with mRNA expression of elongases and desaturases in adipose tissue (all P<0.05). In line with this the estimated activity of elongase (18:1 n-7/16:1 n-7) increased significantly after obesity surgery in all lipid fractions (all P<4 × 10−7) and the increase in the estimated activity of D5D in triglycerides was associated with higher weight loss (r=0.415, P<2 × 10−6). Changes in serum FA profile were similar after obesity surgery and lifestyle intervention, except for the change in the absolute amounts of n-3 FAs between the two studies (P=0.044).Conclusions:Beneficial changes in serum and adipose tissue FAs after obesity surgery could be associated with changes in endogenous metabolism and diet.

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“…While inconsistent, these data collectively support the notion that at least acutely, weight loss does not attenuate inflammatory processes in adipose tissue, in part at least due to the efflux and associated trafficking of lipids [51]. Furthermore, existing evidence suggests that a period of weight stabilization may be required before reductions in pro-inflammatory cytokine gene expression, as well as immune cell number, can consistently be observed [27, 49, 52, 53]. …”

Section: Discussionmentioning

confidence: 69%

The short-term and long-term effects of bariatric/metabolic surgery on subcutaneous adipose tissue inflammation in humans

et al. 2017

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Context The mechanisms mediating the short- and long-term improvements in glucose homeostasis following bariatric/metabolic surgery remain incompletely understood. Objective To investigate whether a reduction in adipose tissue inflammation plays a role in the metabolic improvements seen after bariatric/metabolic surgery, both in the short-term and longer-term. Design Fasting blood and subcutaneous abdominal adipose tissue were obtained before (n=14), at one month (n=9), and 6–12 months (n=14) after bariatric/metabolic surgery from individuals with obesity who were not on insulin or anti-diabetes medication. Adipose tissue inflammation was assessed by a combination of whole-tissue gene expression and flow cytometry-based quantification of tissue leukocytes. Results One month after surgery, body weight was reduced by 13.5±4.4 kg (p<0.001), with improvements in glucose tolerance reflected by a decrease in area-under-the-curve (AUC) glucose in 3-h oral glucose tolerance tests (−105±98 mmol/L*min; p=0.009) and enhanced pancreatic β-cell function (insulinogenic index: +0.8±0.9 pmol/mmol; p=0.032), but no change in estimated insulin sensitivity (Matsuda insulin sensitivity index [ISI]; p=0.720). Furthermore, although biomarkers of systemic inflammation and pro-inflammatory gene expression in adipose tissue remained unchanged, the number of neutrophils increased in adipose tissue 15–20 fold (p<0.001), with less substantial increases in other leukocyte populations. By the 6–12 month follow-up visit, body weight was reduced by 34.8±10.8 kg (p<0.001) relative to baseline, and glucose tolerance was further improved (AUC glucose −276±229; p<0.001) along with estimated insulin sensitivity (Matsuda ISI: +4.6±3.2; p<0.001). In addition, improvements in systemic inflammation were reflected by reductions in circulating C-reactive protein (CRP; −2.0±5.3 mg/dL; p=0.002), and increased serum adiponectin (+1,358±1,406 pg/mL; p=0.003). However, leukocyte infiltration of adipose tissue remained elevated relative to baseline, with pro-inflammatory cytokine mRNA expression unchanged, while adiponectin mRNA expression trended downward (p=0.069). Conclusion Both the short- and longer-term metabolic improvements following bariatric/metabolic surgery occur without significant reductions in measures of adipose tissue inflammation, as assessed by measuring the expression of genes encoding key mediators of inflammation and by flow cytometric immunophenotyping and quantification of adipose tissue leukocytes.

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Effect of Roux-en-Y gastric bypass-induced weight loss on the transcriptomic profiling of subcutaneous adipose tissue

Cited by 23 publications

References 30 publications

Role of metabolism during viral infections, and crosstalk with the innate immune system

Role of metabolism during viral infections, and crosstalk with the innate immune system

Alterations in fatty acid metabolism in response to obesity surgery combined with dietary counseling

The short-term and long-term effects of bariatric/metabolic surgery on subcutaneous adipose tissue inflammation in humans

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