Effect of roundup® (glyphosate formulation) in the energy metabolism and reproductive traits of Hyalella castroi (Crustacea, Amphipoda, Dogielinotidae)

Abstract

Roundup(®) (glyphosate formulation) is a nonselective and posts emergent herbicide used for controlling aquatic weeds and different concentrations are used in cultures around the world. The objective of this investigation was to examine the effects of Roundup(®) (glyphosate formulation) on the biochemical composition, levels of lipoperoxidation, Na(+)/K(+)ATPase activity and reproductive traits in the Hyalella castroi. Amphipods were collected in summer 2009, in the southern Brazilian highlands. In the laboratory, the animals were kept in aquariums under controlled conditions for 7 days, and after this period they were exposed to 0.36, 0.52, 1.08 and 2.16 mg/l of glyphosate for 7 days. After the period of exposure, the animals were immediately frozen for determination of glycogen, proteins, lipids, triglycerides, cholesterol, levels of lipoperoxidation, and Na(+)/K(+)ATPase activity. During each day of the cultivation reproductive traits (number of reproductive pairs, ovigerous females and eggs in the marsupium) were observed. All concentrations of Roundup(®) induced significant decreases in all biochemical parameters and Na(+)/K(+)ATPase activity, and significant increase in lipoperoxidation levels. Showing this form a potentially toxic effect at very low concentrations, this pattern of results can lead to significant changes in trophic structure of limnic environments because these amphipods are important links in food chain in these habitats.

Population structure of two freshwater amphipods (Crustacea: Peracarida: Hyalellidae) from southern Brazil

“…Besides, Castiglioni and Bond-Buckup (2008a) observed a continuous reproduction for H. pleoacuta and H. castroi, but more intense in winter and fall, respectively. Food availability (Xinqing et al, 2013) and quality (Dutra et al, 2011) influenced the reproductive capacity of individuals, causing abundance fluctuations. In those months that the temperature was higher, there was a decrease in water volume and an increase in macrophyte abundance (personal observation), providing a suitable environment for the species reproduction.…”

Effects of sublethal doses of glyphosate on honeybee navigation

“…A negative effect has also been reported in amphibians after chronic exposure to different concentrations of glyphosate (3.8-18 mg l −1 ; Howe et al, 2004;Relyea, 2005a,b). Despite these findings and others that report negative and lethal effects on invertebrates such as amphipods (Dutra et al, 2011), the sublethal impacts of GLY on non-target organisms such as insect pollinators have so far been poorly evaluated (Herbert et al, 2014;Thompson et al, 2014). In this study, we used sublethal concentrations of GLY ranging from 2.5 to 10 mg l −1 .…”

Impact of field‐realistic doses of glyphosate and nutritional stress on mosquito life history traits and susceptibility to malaria parasite infection

“…In other aquatic invertebrate species, this molecule tends to have more significant effect. Glyphosate exposure, even at low concentration (0.1–0.05 mg/L), induced a reduction of juvenile size in the planktonic crustacean Daphnia magna (Cuhra, Traavik, & Bøhn, 2013) and negatively impacted the survival rate in the amphipod Hyalella castroi and the crayfish Cherax quadrinatus (Avigliano, Fassiano, Medesani, Ríos de Molina, & Rodríguez, 2014; Dutra, Fernandes, Failace, & Oliveira, 2011). This suggests that mosquito larvae are more tolerant to glyphosate than other aquatic invertebrate species.…”