Effect of Rosmarinic Acid and Alcohol on Fat Graft Survival in Rat Model

Cin, Baris; Çiloğlu, Nesibe Sinem; Omar, Shahruk; Terzi, Neslihan Kaya

doi:10.1007/s00266-019-01519-x

Cited by 7 publications

(6 citation statements)

References 28 publications

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Section: Discussionsupporting

confidence: 84%

“…In contrast, the ethanol group had a higher survival rate of 73%, and the rosmarinic acid group, which used another plant-based antioxidant substance, showed an even higher survival rate of 82%. 45 Our results are comparable to these studies, and potential use of these agents to improve fat graft viability can be further investigated. Many theories exist regarding fat graft survival, including the one proposed by Eto et al, in which the authors discuss that adipocyte death occurs mostly in the first 24 hours after fat graft transplantation.…”

Section: Plastic and Reconstructive Surgery • June 2024supporting

confidence: 84%

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Increased Total Antioxidant Capacity Value Improves Survival of Fat Grafts in Rat Model

Cinar

Yalcin

Ayas

et al. 2023

Plastic &Amp; Reconstructive Surgery

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Background: While studies aiming to increase fat graft survival continue, in this study, we aimed to investigate the effects of different antioxidants on total antioxidant capacity and their effect on graft survival. Methods: Thirty-two male Wistar rats were divided into four equally sized groups, including a control group and three antioxidant groups receiving either Melatonin (10mg/kg), Zinc (2mg/kg), or Vitamin E and C (100mg/kg). Autologous fat grafts (1.7± 0.4gr) were transferred to the dorsal subcutaneous region, and total antioxidant capacity was measured on day 0 and 1, week 1 and monthly until the third month. Transferred graft volume and mass (1.3± 0.4gr) were measured using the liquid overflow method and precision scales at the end of the study. Routine hematoxylin-eosin staining and immunohistochemistry against perilipin were performed for semiqualitative analysis and h-score for viable adipose cells, respectively. Results: Collected fat grafts measured significantly less in weight and volume and the survival rate was lower in the control group (p<0.01). Control group exhibited a reduction in TAC, while all groups receiving antioxidants had an increase in TAC during the first week (p=0.02, 0.008, and 0.004 for melatonin, zinc, and vitamins, respectively). Immunohistochemistry of the antioxidant group demonstrated a statistically significant excess and reactivity of cells staining with perilipin antibodies. Conclusions: In this animal study, the beneficial effect of antioxidants on fat graft survival can be related to the significant increase in TAC following the first week of their administration.

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Section: Discussionsupporting

confidence: 84%

Section: Plastic and Reconstructive Surgery • June 2024supporting

confidence: 84%

Increased Total Antioxidant Capacity Value Improves Survival of Fat Grafts in Rat Model

Cinar

Yalcin

Ayas

et al. 2023

Plastic &Amp; Reconstructive Surgery

View full text Add to dashboard Cite

show abstract

“…We read with great interest the article entitled ''Effect of Rosmarinic Acid and Alcohol on Fat Graft Survival in Rat Model'' by Cin B et al [1] in Aesthetic Plastic Surgery. In this article, the authors conducted a preclinical study on the effects of rosmarinic acid on fat graft survival in the rat model for autologous fat grafting.…”

Section: Sirmentioning

confidence: 99%

Letter on Effect of Rosmarinic Acid and Alcohol on Fat Graft Survival in Rat Model

Hua

Wei

2020

Aesth Plast Surg

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“…The fat graft survival is affected by the donor and recipient site of the graft and the processes applied during the grafting procedure [11][12][13][14] . To reduce the RR, an environment with wellestablished vascularity and less inflammation and apoptosis should be provided 14,15 .…”

Section: Introductionmentioning

confidence: 99%

“…[11][12][13][14] To reduce the RRs, an environment with well-established vascularity and less inflammation and apoptosis should be provided. 14,15 The nicotinamide adenine dinucleotide phosphate oxidases (NOXs), multi-unit enzymes in many cells, are a substantial origin of the reactive oxygen radicals (ROSs). 16 ROSs cause cell damage and apoptosis by damaging DNA, RNA, and structural elements of the cell.…”

mentioning

confidence: 99%

The Effect of Locally Delivered Apocynin on Fat Graft Survival in an Experimental Rat Animal Model

2022

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Objectives: We aimed to investigate the efficacy of locally delivered apocynin on fat graft survival in an experimental autologous fat grafting (AFG) model created in rats. Methods: Twenty-one Wistar albino male rats were included in this study. The 0.647 g mean weight grafts were harvested from the inguinal region and transferred to the nape of every rat. The subjects were randomly separated into three groups. Saline, dimethyl sulfoxide (DMSO) and apocynin, a dose of 20 mg/kg, solutions were applied once a day for 2 weeks. After 3 months, the rats were sacrificed. The evaluation of physical measurements (weight and volume) and survival rates of the grafts for volume (SRV) and weight (SRW), the viable cell count (VC) with the MTT assay, and histopathological parameters were done. Results: All biophysical parameters were found to be significantly higher in the apocynin group compared to other groups (p < .05). In the MTT test, the saline group was normalized to 100%. According to this, DMSO and apocynin groups’ means were 106% and 163%, respectively. The VC was significantly higher in the apocynin group than the other groups (p < .05). The VC was significantly higher in the DMSO group than in the saline group (p < .05). No significant difference was found in other comparisons performed according to biophysical and histopathological parameters (p > .05). Conclusion: The locally delivered apocynin decreases fat graft volume loss in an experimental AFG model. Consequently, apocynin can be used as an effective substance to increase graft survival.

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Effect of Rosmarinic Acid and Alcohol on Fat Graft Survival in Rat Model

Cited by 7 publications

References 28 publications

Increased Total Antioxidant Capacity Value Improves Survival of Fat Grafts in Rat Model

Increased Total Antioxidant Capacity Value Improves Survival of Fat Grafts in Rat Model

Letter on Effect of Rosmarinic Acid and Alcohol on Fat Graft Survival in Rat Model

The Effect of Locally Delivered Apocynin on Fat Graft Survival in an Experimental Rat Animal Model

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