Effect of Rocket (Eruca sativa) Extract on MRSA Growth and Proteome: Metabolic Adjustments in Plant-Based Media

Doulgeraki, Agapi I.; Efthimiou, Georgios; Paramithiotis, Spiros; Pappas, Katherine M.; Typas, Milton A.; Nychas, George‐John E.

doi:10.3389/fmicb.2017.00782

Cited by 8 publications

(6 citation statements)

References 55 publications

(69 reference statements)

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“…[71]. It was found that extracts of E. sativa seeds had a remarkable antibacterial action against S. aureus and B. cereus [72]. Eruca seed oil also exerts antibacterial and antifungal effects [73].…”

Section: Antioxidant Effectmentioning

confidence: 99%

Eruca Sativa Linn.: Pharmacognostical and Pharmacological Properties and Pharmaceutical Preparations

Jaafar

Jaafar²

2019

Asian J Pharm Clin Res

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Eruca sativa (jarjeer) is an annual herb (family Brassicaceae), which contains a wide range of chemicals and minerals with nutraceutical and organoleptic characteristics. Jarjeer was generally used as a food and traditionally mainly consumed due to its aphrodisiac properties. This crop known to contain various phytochemicals such as flavonoids, phenolic acids, terpens, carotenoids, tannins, glycosides, saponins, sterols, alkaloids, and other secondary metabolites. In leaves, kaempferol and its derivatives, glucosativin, are the main flavonoids and glucosinolate, respectively, while erucic acid and glucoerucin are the main fatty acid and glucosinolate, respectively. Medicinally, the plant has antibacterial, antidiabetic, antihypertensive, antiplatelet, and antioxidant activity and stimulates hair growth and other effects. Trails on topical pharmaceutical preparations involve the use of E. sativa which had been done. These preparations include creams and waxs which are intended to be used for potentiating hair growth and skin fungal and bacterial infection.

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Section: Antioxidant Effectmentioning

confidence: 99%

Eruca Sativa Linn.: Pharmacognostical and Pharmacological Properties and Pharmaceutical Preparations

Jaafar

Jaafar²

2019

Asian J Pharm Clin Res

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“…Interestingly, a study by Doulgeraki et al . [ 46 ] investigated the growth of methicillin-resistant Staphylococcus aureus in a watery rocket salad extract and found an inhibitory effect early in the growth phase.…”

Section: Discussionmentioning

confidence: 99%

“…However, for any compound to be effective in the humid environment of a salad washing factory, high water solubility is a prerequisite. Interestingly, a study by Doulgeraki et al [46] investigated the growth of methicillin-resistant Staphylococcus aureus in a watery rocket salad extract and found an inhibitory effect early in the growth phase.…”

Section: Constituents In the Anti-listerial Rocket Salad Fractionmentioning

confidence: 99%

Anti-listerial properties of chemical constituents of Eruca sativa (rocket salad): From industrial observation to in vitro activity

et al. 2021

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The frequency of foodborne outbreaks epidemiologically associated with Listeria monocytogenes in fresh produce has increased in recent years. Although L. monocytogenes may be transferred from the environment to vegetables during farming, contamination of food products most commonly occurs in food processing facilities, where L. monocytogenes has the ability to establish and persist on processing equipment. The current study was undertaken to collect data on the occurrence of L. monocytogenes and the identity of the endogenous microbiota in a fresh produce processing facility, for which information has remained scarce. L. monocytogenes was not detected in the facility. Experiments simulating conditions in the processing environment were performed, including examination of bacterial growth in nutrients based on vegetables (salad juice) compared to in other types of nutrients (fish, meat). Results showed that the endogenous microbiota (dominated by Pseudomonas) grew well in iceberg lettuce and rocket salad juice at low temperatures, while growth inhibition of L. monocytogenes was observed, particularly in rocket salad juice. The anti-listerial activity in rocket salad juice was retained in a polar chromatographic fraction containing several metabolites. Characterization of this active fraction, using LC-MS/MS, led to identification of 19 compounds including nucleosides and amino acids. Further work is necessary to determine the molecular mechanism responsible for the inhibitory activity of rocket salad constituents. The study nevertheless suggests that the available nutrients, as well as a low temperature (3 °C) and the in-house bacterial flora, may influence the prevalence of L. monocytogenes in fresh produce processing facilities.

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“…The current antimicrobial activities of the leaves and sprouts extracts may attributed to the presence of bioactive compounds such as erucic acid, Novel Research in Microbiology Journal, 2020 isothiocyanates and (-) vebrenon in these extracts, in agreement with the previous findings of Khoobchandani et al, (2010);Paraschos et al, (2011). Recently, Doulgeraki et al, (2017) study showed that there is a notable effect of E. sativa seeds on the antibacterial potential against S. aureus and B. cereus. In addition, Arribas et al, (2019) demonstrated that the presence tocopherols, oryzanol and β-sitosterol in the rice bran are mainly related to the antimicrobial efficacy of rice.…”

Section: Antimicrobial Potential Of E Sativa Leaves and Sprouts Ethamentioning

confidence: 99%

Assessment of the effects of different extraction methods on the phytochemicals, antimicrobial and anticancer activities of Eruca sativa extracts

Ahmad

Shehta

2020

Novel Research in Microbiology Journal

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The present study aimed to investigate the benefits of ultrasonic method of extraction compared to maceration method, on intensifying the phytochemicals, antimicrobial, and cytotoxicity activities of Eruca sativa leaves and sprouts ethanolic extracts. The ultrasonic treatments of E. sativa leaves and sprout, were tested after 10, 20 and 30 min., whereas, maceration treatments of E. sativa leaves and sprout, were considered after 72 h. Results of Gas Chromatography-Mass Spectrometry (GC-MS) analysis showed that E. sativa leaves and sprouts are reach sources of active components such as phenols and flavonoids. Moreover, the sprout macerated extracts showed higher total antioxidant activity (TAA), total phenolic contents (TPC) and total flavonoids (TFC), compared to those of the sprout ultrasonic extracts. On the contrary, the ultrasonic extraction of leaves was more efficient than maceration. Results of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity showed that ultrasonic treated leaves, sprouts (10 min.), and macerated sprouts (72 h), recorded the lowest effective concentrations (EC 50) of; 2.77, 3.846, and 3.676 mg/100 ml, respectively. The antimicrobial activity of the extracts was assessed using the well diffusion method. Results showed that ultrasonic treated sprouts (10 min.) and ultrasonic treated leaves (10 and 30 min.) have better antifungal activity against Aspergillus fumigatus (recording diameter of inhibition zone of 13 mm) and Candida albicans (recording diameters of inhibition zones of 13 and 11 mm, respectively). For bacteria, the Staphylococcus aureus was effectively inhibited (recording inhibition zone diameter of 13 mm) by the ultrasonic treated leaves (20 min.), Bacillus cereus was effectively inhibited with ultrasonic treated sprouts (20 and 30 min., both recording 13 mm), ultrasonic treated leaves (10 and 20 min., recording 14, 13 mm, respectively), and the macerated leaves (72 h, recording 13 mm). Salmonella typhimurium recorded (13 mm) with ultrasonic treated leaves (10 min.), and (12 mm) with macerated sprouts. Results of the cytotoxic potency demonstrated high activity of the macerated sprouts on HepG2 cell lines, whereas the macerated sprouts and ultrasonic treated leaves (30 min.) presented high efficacy on the HT-29 cells. Thus we concluded that extracts of E. sativa leaves and sprouts produced using different extraction methods, have a substantial beneficial antioxidant, antimicrobial and anticancer activities.

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Effect of Rocket (Eruca sativa) Extract on MRSA Growth and Proteome: Metabolic Adjustments in Plant-Based Media

Cited by 8 publications

References 55 publications

Eruca Sativa Linn.: Pharmacognostical and Pharmacological Properties and Pharmaceutical Preparations

Eruca Sativa Linn.: Pharmacognostical and Pharmacological Properties and Pharmaceutical Preparations

Anti-listerial properties of chemical constituents of Eruca sativa (rocket salad): From industrial observation to in vitro activity

Assessment of the effects of different extraction methods on the phytochemicals, antimicrobial and anticancer activities of Eruca sativa extracts

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