Effect of RIP Overexpression on Abiotic Stress Tolerance and Development of Rice

Abstract

Ribosome-inactivating proteins (RIPs) are a class of cytotoxic enzymes that can inhibit protein translation by depurinating rRNA. Most plant RIPs are synthesized with a leader sequence that sequesters the proteins to a cell compartment away from the host ribosomes. However, several rice RIPs lack these signal peptides suggesting they reside in the cytosol in close proximity to the plant ribosomes. This paper aims to elucidate the physiological function of two nucleocytoplasmic RIPs from rice, in particular, the type 1 RIP referred to as OsRIP1 and a presumed type 3 RIP called nuRIP. Transgenic rice lines overexpressing these RIPs were constructed and studied for developmental effects resulting from this overexpression under greenhouse conditions. In addition, the performance of transgenic seedlings in response to drought, salt, abscisic acid and methyl jasmonate treatment was investigated. Results suggest that both RIPs can affect methyl jasmonate mediated stress responses.

Environmental Stress and Plants

“…The distinct roles of UGT74E2 indicate different regulatory networks between Arabidopsis and rice. Rice is also used in the study of Wytynck et al [15] to investigate the role of ribosome-inactivating proteins (RIPs). RIPs are enzymes capable of irreversible modification of ribosomal RNAs.…”

Physiological and molecular implications of multiple abiotic stresses on yield and quality of rice

“…He et al (2018) reported that ABA treatment mimicked protein perturbations in rice subjected to combined salinity stress and desiccation. In another study, Wytynck et al, 2021 reported similar ultrastructural changes in young leaf cells of rice seedlings subjected to salinity or high temperature stress, including the enhanced formation of rough endoplasmic reticulum assembly, reduced cristae formation in mitochondria, and disorganized cell wall fibrils.…”

The Structural Characterization and Antipathogenic Activities of Quinoin, a Type 1 Ribosome-Inactivating Protein from Quinoa Seeds

“…The pioneering work of Stirpe et al reported that the increase in the inhibitory activity of protein synthesis and activity on DNA is due to the presence of RIPs in the leaves of both Hura crepitans L. and P. americana when subjected to heat or osmotic stress [38]. Since then, experimental evidence has confirmed the control of the expression of RIPs at the gene level during abiotic and biotic stress in plants [39,40]. The de novo assembly of the pokeweed (P. americana) genome showed that the genes of both the pokeweed antiviral protein (PAP) and the PAP isoforms exhibited a long 5 UTR containing one intron and cis-regulatory elements associated with diverse biotic and abiotic stresses [41].…”