Effect of rifampin resistance on nodulating competitiveness of <i>Rhizobium meliloti</i>

Lewis, D. M.; Bromfield, E. S. P.; Barran, L. R.

doi:10.1139/m87-059

Cited by 23 publications

(9 citation statements)

References 10 publications

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“…(2, 12, 13) and Rhizobium spp. (10,29). These studies demonstrated that chromosomally mediated rifampin resistance does not affect the colonizing ability of strain WCS358, confirming the results of Glandorf et al (19).…”

Section: Discussionsupporting

confidence: 87%

Siderophore receptor PupA as a marker to monitor wild-type Pseudomonas putida WCS358 in natural environments

Raaijmakers

Bitter

Punte

et al. 1994

Appl Environ Microbiol

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For application of genetically engineered fluorescent Pseudomonas spp., specific markers are required for monitoring of wild-type Pseudomonas strains and their genetically modified derivatives in natural environments. In this study, the specific siderophore receptor PupA of plant growth-promoting Pseudomonas putida WCS358 was used as a marker to monitor wild-type strain WCS358. After introduction into natural soil and rhizosphere environments, strain WCS358 could be recovered efficiently on a medium amended with 300 ,IM pseudobactin 358. Although low population densities of indigenous pseudomonads ('103/g of soil or root) were recovered on the pseudobactin 358-amended medium, subsequent agglutination assays with a WCS358-specific polyclonal antiserum enabled accurate monitoring of populations of wild-type strain WCS358 over a range of approximately 103 to 107 CFU/g of soil or root. Genetic analysis of the background population by PCR and Southern hybridization revealed that natural occurrence of the pupA gene was limited to a very small number of indigenous Pseudomonas spp. which are very closely related to P. putida WCS358. The PupA marker system enabled the study of differences in rhizosphere colonization among wild-type strain WCS358, rifampinresistant derivative WCS358rr, and Tn5 mutant WCS358::xylE. Chromosomally mediated rifampin resistance did not affect the colonizing ability of P. putida WCS358. However, Tn5 mutant WCS358:xylE colonized the radish rhizosphere significantly less than did its parental strain.

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Section: Discussionsupporting

confidence: 87%

Siderophore receptor PupA as a marker to monitor wild-type Pseudomonas putida WCS358 in natural environments

Raaijmakers

Bitter

Punte

et al. 1994

Appl Environ Microbiol

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“…Selection of chromosomal-mediated antibiotic resistance often results in associated genetic changes which influence ecologically important traits (Andrews, 1986). Rifampicin resistance has been reported to diminish symbiotic effectiveness (Pankhurst, 1977) and nodulating competitiveness (Lewis et al, 1987) of Rhizobium spp. Davies and Whitbread (1989) reported an increase in the number of nonfluorescent colonies when Rift mutants of strains of fluorescent Pseudomonas were cultured in liquid KB medium in the presence of iron.…”

Section: Discussionmentioning

confidence: 99%

“…However, it cannot be excluded that rifampicin resistance can change characteristics in the Pseudomonas strains used, or is unstable under field conditions. Among Rhizobium spp., spontaneous rifampicin resistance (Rift) was associated with diminished symbiotic effectiveness and nodulating competitiveness (Lewis et al, 1987;Pankhurst, 1977). Certain Rift mutants of P. fluorescens were less able to compete with the parental strain in sterile soil (Compeau et al, 1988).…”

Section: Introductionmentioning

confidence: 99%

Stability of rifampicin resistance as a marker for root colonization studies of Pseudomonas putida in the field

et al. 1992

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The stability of rifampicin resistance in plant growth-promoting Pseudornonas putida strain WCS358 was studied in potato rhizosphere in the field. Three out of seven rifampicin-resistant mutants of strain WCS358 were selected in this study. Their specific growth rate, competitive growth in liquid medium and colonization of potato roots in non-sterile soil, was comparable to that of their parental strain. These rifampicin-resistant mutants were used to treat potato seed tubers, which were thereafter sown in the field. To test the stability of the rifampicin resistance in the field, about 1200 fluorescent Pseudomonas isolates obtained from underground plant parts at 82, 95,109 and 130 days after seeding, were tested for rifampicin resistance and for agglutination with an antiserum specific for strain WCS358. All fluorescent Pseudomonas isolates that showed a positive agglutination reaction with the antiserum, were also rifampicin-resistant. Twelve agglutination-positive isolates, selected at random, were all identified as strain WCS358 from patterns of lipopolysaccharides after sodium dodecyl sulphatepolyacrylamide gel electrophoresis. Thus, rifampicin resistance seems to be a stable marker in the mutants of strain WCS358 tested, also under field conditions. It is concluded that rifampicin resistance can be used as a reliable marker for ecological studies on rhizosphere pseudomonads.

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“…Therefore, mutation to streptomycin and erythromycin resistance did not adversely affect the symbiotic N 2 -fixation system in these strains. Although similarity in N 2 -fixation capacity between spontaneous antibiotic-resistant mutants and (brady)rhizobia parental strains has been shown by others (Gollobin and Levin 1974;Levin and Montgomery 1974;Lewis et al 1987), this is not always the case (Bromfield and Jones 1979;Date and Hurse 1992;Pankhurst 1977;Schwinghamer and Dudman 1973;Turco et al 1986). Therefore, evaluation of symbiotic properties is essential before a mutant can be used to represent its parent in ecological studies.…”

Section: Symbiotic Efficiency Of Mutantsmentioning

confidence: 91%

Using spontaneous antibiotic-resistant mutants to assess competitiveness of bradyrhizobial inoculants for nodulation of soybean

Ramirez¹,

Israel²,

Wollum³

1998

Can. J. Microbiol.

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Spontaneous mutants (3/parental strain) of soybean bradyrhizobia resistant to streptomycin and erythromycin were selected from strains isolated from bradyrhizobial populations indigenous to Cape Fear and Dothan soils. These were used to evaluate (i) the validity of using antibiotic-resistant mutants to make inferences about the competitiveness of parental strains in soil environments and (ii) the recovery of strains in nodules after inoculation of soybeans grown in soils with indigenous bradyrhizobial populations. Streptomycin and erythromycin resistances of all mutants were stable after approximately 27 generations of growth in yeast extract -mannitol medium, but 33% of the mutants lost resistance to erythromycin upon passage through nodules. Only 17% of the mutants were as competitive as their parental strain when inoculated in a ratio near 1:1 in vermiculite. Four of 10 mutants, which differed in competitiveness from their parental strain in vermiculite, had competitiveness against the soil populations equal to that of their parental strain. Therefore, assessment of competitiveness of mutants and parental strains in non-soil media may not accurately reflect their competitiveness in soil systems. For both the Cape Fear and Dothan soils, recovery of a given mutant from nodules of field-grown plants was always lower than from nodules of plants grown in the greenhouse. Inoculation of the entire rooting zone in the greenhouse experiment and of only a portion of the rooting zone in the field experiments may account for this difference in recovery. Techniques that increase the volume of soil inoculated may enhance nodulation by inoculant strains.Résumé : Des mutants spontanés (3/souche parentale) de bradyrhizobiums du soja résistants à la streptomycine et à l'érythromycine ont été sélectionnés de populations indigènes de sols de Cape Fear et de Dothan. Ces mutants ont été utilisés pour (i) valider l'utilisation de mutants résistants aux antibiotiques pour établir la compétitivité des souches parentales dans les sols et (ii) évaluer la récupération de souches dans les nodules après inoculation de fèves de soja dans les sols avec des populations indigènes de bradyrhizobiums. Chez tous les mutants, la résistance à la streptomycine et à l'érythromycine demeurait stable après environ 27 générations dans un milieu à l'extrait de levure avec mannitol, mais 33% des mutants perdaient leur résistance à l'érythromycine suite à un passage à travers les nodules. Seulement 17% des mutants étaient aussi compétitifs que la souche parentale lorsqu'inoculés dans de la vermiculite dans un rapport de l'ordre de l:l. Quatre des 10 mutants qui avaient une compétitivité différente de leur souche parentale dans la vermiculite, se sont montrés compétitifs contre des populations dans le sol comparables à celles de leur souche parentale. En conséquence, l'évaluation de la compétitivité des mutants et des souches parentales dans les milieux exempts de sol pourrait ne pas refléter adéquatement leur compétitivité dans les systèmes contenant du so...

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Effect of rifampin resistance on nodulating competitiveness of Rhizobium meliloti

Cited by 23 publications

References 10 publications

Siderophore receptor PupA as a marker to monitor wild-type Pseudomonas putida WCS358 in natural environments

Siderophore receptor PupA as a marker to monitor wild-type Pseudomonas putida WCS358 in natural environments

Stability of rifampicin resistance as a marker for root colonization studies of Pseudomonas putida in the field

Using spontaneous antibiotic-resistant mutants to assess competitiveness of bradyrhizobial inoculants for nodulation of soybean

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