Effect of rice-expressed amyloid β in the Tg2576 Alzheimer's disease transgenic mouse model

Nojima, Jun; Maeda, Azusa; Aoki, Sho; Suo, Satoshi; Yanagihara, Dai; Watanabe, Yoshihisa; Yoshida, Taiji; Ishiura, Shoichi

doi:10.1016/j.vaccine.2011.06.073

Cited by 21 publications

(19 citation statements)

References 24 publications

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“…A correlation between Aβ accumulation in the brain and the number of entries was found in previous experiments. 19) Furthermore, increase in the number of entries was observed when the rats got damage in the entorhinal cortex. 34) However, there was no difference between SAMP8 mice-fed WT rice and SAMP8 mice-fed Aβ rice, or between SAMP8 and SAMR1 mice (Fig.…”

Section: Spontaneous Alternation In a Y-maze Testmentioning

confidence: 99%

“…16) We previously developed a rice vaccine, 17,18) and immunized familial AD model mice (Tg2576); as a result, the antibody titer increased and Aβ accumulation in the mouse brain was prevented compared with Tg2576-fed wild type (WT) rice. 19) In this study, we immunized senescence-accelerated mouse prone 8 (SAMP) mice, a sporadic AD model, 20) with the rice vaccine. SAMP8 is one of the strains in the senescence-accelerated mice (SAM).…”

Section: )mentioning

confidence: 99%

“…4) statistical software. 19) Quantification of Aβ in the brain by sandwich ELISA. The mouse brains were harvested.…”

Section: )mentioning

confidence: 99%

“…Thus, we quantified the accumulation of Aβ40. A standard peptide and the samples were incubated in microplates coated with the monoclonal antibody BNT77, which recognizes human and rat Aβ (11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28), for 1 h at 4°C. After washing five times, the plates were incubated with an HRP-conjugated antibody raised against the C-terminus of Aβ40 for 1 h at 4°C.…”

Section: )mentioning

confidence: 99%

“…Alternation rate (%) was calculated as the number of actual alternations performed divided by the number of possible alternations (defined as the number of arm entries minus two) multiplied by 100. [19][20][21][22][23][24][25][26][27][28][29][30] Immunostaining of SAMP8, SAMR1, and Tg2576 mouse brain slices. The remaining brain hemispheres were fixed in 10% formaldehyde and then embedded in paraffin.…”

Section: Spontaneous Alternation In a Y-maze Testmentioning

confidence: 99%

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Aβ induces oxidative stress in senescence-accelerated (SAMP8) mice

Takagane

Nojima

Mitsuhashi

et al. 2015

Bioscience, Biotechnology, and Biochemistry

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According to the amyloid hypothesis, amyloid β accumulates in brains with Alzheimer’s disease (AD) and triggers cell death and memory deficit. Previously, we developed a rice Aβ vaccine expressing Aβ, which reduced brain Aβ levels in the Tg2576 mouse model of familial AD. We used senescence-accelerated SAMP8 mice as a model of sporadic AD and investigated the relationship between Aβ and oxidative stress. Insoluble Aβ and 4-hydroxynonenal (4-HNE) levels tended to be reduced in SAMP8 mice-fed the rice Aβ vaccine. We attempted to clarify the relationship between oxidative stress and Aβ in vitro. Addition of Aβ peptide to the culture medium resulted in an increase in 4-HNE levels in SH-SY5Y cells. Tg2576 mice, which express large amounts of Aβ in their brain, also exhibited increased 4-HNE levels; this increase was inhibited by the Aβ vaccine. These results indicate that Aβ induces oxidative stress in cultured cells and in the mouse brain.

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Section: Spontaneous Alternation In a Y-maze Testmentioning

confidence: 99%

Section: )mentioning

confidence: 99%

“…4) statistical software. 19) Quantification of Aβ in the brain by sandwich ELISA. The mouse brains were harvested.…”

Section: )mentioning

confidence: 99%

Section: )mentioning

confidence: 99%

Section: Spontaneous Alternation In a Y-maze Testmentioning

confidence: 99%

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Aβ induces oxidative stress in senescence-accelerated (SAMP8) mice

Takagane

Nojima

Mitsuhashi

et al. 2015

Bioscience, Biotechnology, and Biochemistry

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Development of the Transgenic Rice Accumulating Flavonoids in Seed by Metabolic Engineering

Ogo

Takaiwa

2017

Phytonutritional Improvement of Crops

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The use of rice seeds to produce human pharmaceuticals for oral therapy

Wakasa

Takaiwa

2013

Biotechnology Journal

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Rice (Oryza sativa L.) is the major staple food consumed by half of the world's population. Rice seeds have gained recent attention as bioreactors for the production of human pharmaceuticals such as therapeutic proteins or peptides. Rice seed production platforms have many advantages over animal cell or microbe systems in terms of cost-effectiveness, scalability, safety, product stability and productivity. Rice seed-based human pharmaceuticals are expected to become innovative therapies as edible drugs. Therapeutic proteins can be sequestered within natural cellular compartments in rice seeds and protected from harsh gastrointestinal environments. This review presents the state-of-the-art on the construction of gene cassettes for accumulation of pharmaceutical proteins or peptides in rice seeds, the generation of transgenic rice plants, and challenges involved in the use of rice seeds to produce human pharmaceuticals.

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Effect of rice-expressed amyloid β in the Tg2576 Alzheimer's disease transgenic mouse model

Cited by 21 publications

References 24 publications

Aβ induces oxidative stress in senescence-accelerated (SAMP8) mice

Aβ induces oxidative stress in senescence-accelerated (SAMP8) mice

Development of the Transgenic Rice Accumulating Flavonoids in Seed by Metabolic Engineering

The use of rice seeds to produce human pharmaceuticals for oral therapy

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