Effect of riboflavin deficiency on sorbitol pathway in rat lens

KIOM-79 inhibits aldose reductase activity and cataractogenesis in Zucker diabetic fatty rats

“…The activity of aldose reductase was assayed according to the methods described above. Sorbitol level in the lens was estimated using an enzymatic method [11] . Briefly, the lens homogenate was centrifuged at 3000 rev/min for 10 min.…”

“…Sorbitol level in the lens was estimated using an enzymatic method. [11] Briefly, the lens homogenate was centrifuged at 3000 rev/min for 10 min. The supernatant was neutralized with 2 m potassium carbonate, centrifuged again, and the resulting supernatant was diluted with distilled water and used for the sorbitol assay.…”