Effect of resveratrol on peritoneal macrophages in rats with severe acute pancreatitis

Ma, Zhenhua; Ma, Qingyong; Wang, L.-C.; Sha, Huanchen; Wu, Shufang; Zhang, M.

doi:10.1007/s00011-005-1388-z

Cited by 70 publications

(44 citation statements)

References 24 publications

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“…The stilbene administered to rats in a single dose or for a few days alleviated the severity of acute pancreatitis (27,28,30,32,45). This action is postulated to be related to the inhibition of the activation of nuclear factor-B (NF-B) and decreased expression of tumor necrosis factor-␣ (TNF-␣) and interleukin-8 (IL-8) (30,32). Furthermore, the reduced damage of the pancreas by reactive oxygen species and diminished pancreatic tissue infiltration of neutrophils in resveratrol-treated rats were also shown (27,28).…”

mentioning

confidence: 99%

Resveratrol-induced inhibition of insulin secretion from rat pancreatic islets: evidence for pivotal role of metabolic disturbances

Szkudelski¹

2007

American Journal of Physiology-Endocrinology and Metabolism

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Resveratrol is a stilbene present in different plant species and exerting numerous beneficial effects, including prevention of diabetes and attenuation of some diabetic complications. Its inhibitory effect on insulin secretion was recently documented, but the exact mechanism underlying this action remains unknown. Experiments employing diazoxide and a high concentration of K+revealed that, in depolarized pancreatic islets incubated for 90 min with resveratrol (1, 10, and 100 μM), insulin secretion stimulated by glucose and leucine was impaired. The attenuation of the insulin secretory response to 6.7 mM glucose was not abrogated by blockade of intracellular estrogen receptors and was found to be accompanied by diminished islet glucose oxidation, enhanced lactate production, and reduced ATP levels. Glucose-induced hyperpolarization of the mitochondrial membrane was also reduced in the presence of resveratrol. Moreover, in depolarized islets incubated with 2.8 mM glucose, activation of protein kinase C or protein kinase A potentiated insulin release; however, under these conditions, resveratrol was ineffective. Further studies also revealed that, under conditions of blocked voltage-dependent calcium channels, the stilbene reduced insulin secretion induced by a combination of glucose with forskolin. These data demonstrate that resveratrol 1) inhibits the amplifying pathway of insulin secretion, 2) exerts an insulin-suppressive effect independently of its estrogenic/anti-estrogenic activity, 3) shifts islet glucose metabolism from mitochondrial oxidation to anaerobic, 4) fails to abrogate insulin release promoted without metabolic events, and 5) does not suppress hormone secretion as a result of the direct inhibition of Ca2+influx through voltage-dependent calcium channels.

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mentioning

confidence: 99%

Resveratrol-induced inhibition of insulin secretion from rat pancreatic islets: evidence for pivotal role of metabolic disturbances

Szkudelski¹

2007

American Journal of Physiology-Endocrinology and Metabolism

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“…Vasoactive mediators, such as bradykinin, platelet activating factor, endothelin and NO, participate in the development of pancreatic microcirculatory failure. Recently, in druginduced pancreatitis models, some researchers found that there is a correlation among NF-kappaB activation, serum amylase, reactive oxygen species level and tissue damage, suggesting that NF-kappaB and iNOS play a key role in the pathogenesis of acute pancreatitis [14][15][16] . After treatment with antioxidants or NOS inhibitors, the levels of myeloperoxidase, serum amylase and NO, as well as iNOS activities are decreased significantly, and the pancreatic inflammation is improved [14,15] .…”

Section: Discussionmentioning

confidence: 99%

“…After treatment with antioxidants or NOS inhibitors, the levels of myeloperoxidase, serum amylase and NO, as well as iNOS activities are decreased significantly, and the pancreatic inflammation is improved [14,15] . Ma et al [16] found that the expression of NF-kappaB and iNOS in peritoneal macrophages is significantly higher in rats with severe acute pancreatitis, and anti-inflammatory agents decrease the expression of TNF-alpha, IL-1 and NO in peritoneal macrophages, reducing the severity of pancreatitis. In FolchPuy's experiment, infusion of a contrast medium into the pancreatic duct could result in an inflammatory process characterized by increased lipase levels in plasma and edema as well as increased myeloperoxidase activity in pancreas, suggesting that activation of NF-kappaB is correlated with iNOS expression in pancreatic cells [17] .…”

Section: Discussionmentioning

confidence: 99%

Protective effect of inducible nitric oxide synthase inhibitor on pancreas transplantation in rats

Bai-Feng¹,

Li²,

Yongfeng³

et al. 2007

WJG

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AIM:To investigate the effect of inducible nitric oxide synthase inhibitor, aminoguanidine, on pancreas transplantation in rats. METHODS:A model of pancreas transplantation was established in rats. Streptozotocin-induced diabetic male Wistar rats were randomly assigned to sham-operation control group (n = 6), transplant control group (n = 6), and aminoguanidine (AG) treatment group (n = 18). In the AG group, aminoguanidine was added to intravascular infusion as the onset of reperfusion at the dose of 60 mg/kg, 80 mg/kg, 100 mg/kg body weight, respectively. Serum nitric oxide (NO) level, blood sugar and amylase activity were detected. Nitric oxide synthase (NOS) test kit was used to detect the pancreas cNOS and inducible NOS (iNOS) activity. Pancreas sections stained with HE and immunohistochemistry were evaluated under a light microscope. RESULTS:As compared with the transplant control group, the serum NO level and amylase activity decreased obviously and the evidence for pancreas injury was much less in the AG group. The AG (80 mg/kg body weight) group showed the most significant difference in NO and amylase (NO: 66.0 ± 16.6 vs 192.3 ± 60.0, P < 0.01 and amylase: 1426 ± 177 vs 4477 ± 630, P < 0.01).The expression and activity of tissue iNOS, and blood sugar in the AG (80 mg/kg body weight) group were much lower than those in the transplant control group (iNOS: 2.01 ± 0.23 vs 26.59 ± 5.78, P < 0.01 and blood sugar: 14.2 ± 0.9 vs 16.8 ± 1.1, P < 0.01). CONCLUSION:Selective iNOS inhibitor, aminoguanidine as a free radical, has a protective effect on pancreas transplantation in rats by inhibiting NO and reducing its toxicity.

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“…It has been shown to possess diverse biochemical and physiological functions, including anti-aging (9), anti-platelet aggregation (201,312), anti-inflammatory (268), cardioprotection (15,116,119), and estrogenic properties (160). Experimental results from animal models have revealed that the anti-inflammatory properties of resveratrol control the development of arthritis (66), pancreatitis (198,199), and colitis (153). Although resveratrol is a poor ROS scavenger in vitro, it behaves as a potent anti-oxidant due to its ability to increase the synthesis of nitric oxide in vivo (22,54,96,324).…”

Section: The Inhibitory Mechanisms Of Natural Compounds Against Npc Smentioning

confidence: 99%

Potential Therapeutic Molecular Targets for Nasopharyngeal Carcinoma

Chen¹

2012

Carcinogenesis, Diagnosis, and Molecular Targeted Treatment for Nasopharyngeal Carcinoma

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Effect of resveratrol on peritoneal macrophages in rats with severe acute pancreatitis

Abstract: The inhibiting effect on the inflammatory response and the decreased expression of TNF-alpha, IL-1 and NO in peritoneal macrophages suggest resveratrol as a novel anti-inflammatory agent for reducing the severity of SAP.

Cited by 70 publications

References 24 publications

Resveratrol-induced inhibition of insulin secretion from rat pancreatic islets: evidence for pivotal role of metabolic disturbances

Resveratrol-induced inhibition of insulin secretion from rat pancreatic islets: evidence for pivotal role of metabolic disturbances

Protective effect of inducible nitric oxide synthase inhibitor on pancreas transplantation in rats

Potential Therapeutic Molecular Targets for Nasopharyngeal Carcinoma

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