“…As the most valuable pollinators for modern agriculture and ecological diversity (The Honeybee Genome Sequencing Consortium, 2006;Klein et al, 2007;Belsky and Joshi, 2019), the development of honeybee populations is influenced by many factors (Potts et al, 2010;Belsky and Joshi, 2019;Denlinger and Yocum, 2020), including environmental temperature (Abou-Shaara et al, 2012. Environmental temperature can affect the internal and external activities of honeybee colonies (Tautz et al, 2003;Abou-Shaara et al, 2012, such as development (Tautz et al, 2003;Groh et al, 2004;Chuda-Mickiewicz and Samborski, 2015;Medina et al, 2020), phenology (Langowska et al, 2017), learnability (Tautz et al, 2003), behavioral performance (Tautz et al, 2003;Al-Qarni, 2006;Blazyte-Cereskiene et al, 2010), and susceptibility to disease (Groh et al, 2004;Conte and Navajas, 2008;Dalmon et al, 2019). And temperature can affect the biochemical and physiological processes of honeybees, including survival, tolerance, loss of body water, and the activity of antioxidant and detoxification enzymes (Dalmon et al, 2019;Li X. Y. et al, 2019).…”