Effect of respiratory inhibitors on mitochondrial complexes and ADP/ATP translocators in the Triticum aestivum roots

Gazizova, N. I.; Rakhmatullina, Daniya; Minibayeva, Farida

doi:10.1016/j.plaphy.2020.04.014

Cited by 8 publications

(11 citation statements)

References 37 publications

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“…The Complex I in-gel NADH-dehydrogenase activity stain was carried out as described by Sabar et al (2005). The bands were identified by comparison to the results of Gazizova et al (2020), who performed their assay on protein complexes isolated from wheat root mitochondria. The gel was rinsed thoroughly with double deionised water (DDI) prior to protein staining with colloidal Coomassie (Eubel et al 2005).…”

Section: Methodsmentioning

confidence: 99%

Mitochondrial Complex I activity is inhibited by changes in the abundance of phosphatidylinositol and phosphatidylserine in wheat plants exposed to high temperatures

Malone

Rupasinghe

Roessner

et al. 2023

Preprint

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We evaluated changes to the lipo-protein network of wheat mitochondria of differing heat tolerance in response to heat shock.Using targeted mass spectrometry, candidate transitions were selected to quantify changes in membrane lipids and the embedded protein components of the electron transport chain, which play a vital role in maintaining respiration.A significant increase in phosphatidylserine was exclusive to the mitochondria of the heat-tolerant wheat cultivar. In the absence of this, the heat-sensitive cultivar displayed a reduced Complex I activity. The minor membrane constituent phosphatidylserine plays a role in conveying thermotolerance, making this membrane lipid a focal point for the breeding of future heat tolerant crops.

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Section: Methodsmentioning

confidence: 99%

Mitochondrial Complex I activity is inhibited by changes in the abundance of phosphatidylinositol and phosphatidylserine in wheat plants exposed to high temperatures

Malone

Rupasinghe

Roessner

et al. 2023

Preprint

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“…As a result, on oxalic acid or phenolic substances, the CUE of soil microbial communities is significantly smaller than on glucose (Frey et al, 2013). Other characteristics, such as whether they block other metabolic pathways (Gazizova, Rakhmatullina & Minibayeva, 2020), where compounds reach central metabolism, whose cell components they are transformed to Gunina et al (2014), and how quickly those components are recycled, are likely to be significant for CUE (Dudley, Karim & Jewett, 2015). Finally, the effect of carbon quality on CUE may be influenced by the bacteria's past nutrient regime; bacteria that have been exposed to carbon-limited settings can metabolize a far broader range of substrates than those that have been exposed to carbon-rich settings (von Stockar & van der Wielen, 2013).…”

Section: Quality Of the Substratementioning

confidence: 99%

Variation of soil microbial carbon use efficiency (CUE) and its Influence mechanism in the context of global environmental change: a review

Adingo

Liu

et al. 2021

PeerJ

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Soil microbial carbon utilization efficiency (CUE) is the efficiency with which microorganisms convert absorbed carbon (C) into their own biomass C, also referred to as microorganism growth efficiency. Soil microbial CUE is a critical physiological and ecological parameter in the ecosystem’s C cycle, influencing the processes of C retention, turnover, soil mineralization, and greenhouse gas emission. Understanding the variation of soil microbial CUE and its influence mechanism in the context of global environmental change is critical for a better understanding of the ecosystem’s C cycle process and its response to global changes. In this review, the definition of CUE and its measurement methods are reviewed, and the research progress of soil microbial CUE variation and influencing factors is primarily reviewed and analyzed. Soil microbial CUE is usually expressed as the ratio of microbial growth and absorption, which is divided into methods based on the microbial growth rate, microbial biomass, substrate absorption rate, and substrate concentration change, and varies from 0.2 to 0.8. Thermodynamics, ecological environmental factors, substrate nutrient quality and availability, stoichiometric balance, and microbial community composition all influence this variation. In the future, soil microbial CUE research should focus on quantitative analysis of trace metabolic components, analysis of the regulation mechanism of biological-environmental interactions, and optimization of the carbon cycle model of microorganisms’ dynamic physiological response process.

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“…Thus, mETC in plants has alternative external and internal NADH DH ( Figure 4B ) that can directly use the matrix or cytosolic NAD(P)H and therefore they can be an alternative electron source. It is believed that these alternative matrix or cytosolic NAD(P)H DH are competing with the activity of Complex I, and some are activated even when Complex I is inhibited ( Gazizova et al., 2020 ). The role of alternative NADH DH is not fully defined; however, they may be linked with plant responses to stress.…”

Section: The Alternative Electron Entry Into Plant Metcmentioning

confidence: 99%

Ascorbate synthesis as an alternative electron source for mitochondrial respiration: Possible implications for the plant performance

et al. 2022

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The molecule vitamin C, in the chemical form of ascorbic acid (AsA), is known to be essential for the metabolism of humans and animals. Humans do not produce AsA, so they depend on plants as a source of vitamin C for their food. The AsA synthesis pathway occurs partially in the cytosol, but the last oxidation step is physically linked to the respiratory chain of plant mitochondria. This oxidation step is catalyzed by l-galactono-1,4-lactone dehydrogenase (l-GalLDH). This enzyme is not considered a limiting step for AsA production; however, it presents a distinguishing characteristic: the l-GalLDH can introduce electrons directly into the respiratory chain through cytochrome c (Cytc) and therefore can be considered an extramitochondrial electron source that bypasses the phosphorylating Complex III. The use of Cytc as electron acceptor has been debated in terms of its need for AsA synthesis, but little has been said in relation to its impact on the functioning of the respiratory chain. This work seeks to offer a new view about the possible changes that result of the link between AsA synthesis and the mitochondrial respiration. We hypothesized that some physiological alterations related to low AsA may be not only explained by the deficiency of this molecule but also by the changes in the respiratory function. We discussed some findings showing that respiratory mutants contained changes in AsA synthesis. Besides, recent works that also indicate that the excessive electron transport vial-GalLDH enzyme may affect other respiratory pathways. We proposed that Cytc reduction by l-GalLDH may be part of an alternative respiratory pathway that is active during AsA synthesis. Also, it is proposed that possible links of this pathway with other pathways of alternative electron transport in plant mitochondria may exist. The review suggests potential implications of this relationship, particularly for situations of stress. We hypothesized that this pathway of alternative electron input would serve as a strategy for adaptation of plant respiration to changing conditions.

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Effect of respiratory inhibitors on mitochondrial complexes and ADP/ATP translocators in the Triticum aestivum roots

Cited by 8 publications

References 37 publications

Mitochondrial Complex I activity is inhibited by changes in the abundance of phosphatidylinositol and phosphatidylserine in wheat plants exposed to high temperatures

Mitochondrial Complex I activity is inhibited by changes in the abundance of phosphatidylinositol and phosphatidylserine in wheat plants exposed to high temperatures

Variation of soil microbial carbon use efficiency (CUE) and its Influence mechanism in the context of global environmental change: a review

Ascorbate synthesis as an alternative electron source for mitochondrial respiration: Possible implications for the plant performance

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