Effect of resin infiltration on the color and microhardness of bleached white‐spot lesions in bovine enamel (an in vitro study)

Horuztepe, Sidika Aynur; Başeren, Meserret

doi:10.1111/jerd.12308

Cited by 32 publications

(47 citation statements)

References 31 publications

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“…Value L* represented the degree of lightness; it could vary from black (0) to white (100). Values a* and b* represented the red (+a*), green (−a*), yellow (+b*), and blue (−b*) degree in the samples [15][16][17]. Each sample was measured at two points in time: when the study began and after the mouth rinse application.…”

Section: Color Evaluationmentioning

confidence: 99%

Effect of mouth rinses on tooth enamel surface

Favaro¹,

Guiraldo²,

Lopes³

et al. 2020

J Oral Sci

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This study aims to evaluate the effect of mouth rinses on the color, microhardness, and surface roughness of tooth enamel. Sixty dental blocks were collected from human third molars and divided in five groups (n = 12): the control group (CG) without immersion in mouth rinse, Listerine Zero, Colgate Plax Fresh Mint, Listerine Whitening, and Colgate Luminous White. The groups were subjected to initial color analysis; the microhardness and roughness of the enamel surfaces were evaluated. Next, the samples were subjected to immersion in mouth rinses or brushing with conventional fluoride toothpaste (CG) according to the manufacturer's instructions; after a 12-week treatment, the color, microhardness, and roughness were once again assessed and compared with the initial analysis. Data were tabulated and analyzed through a two-way analysis of variance (ANOVA) (color and roughness) followed by Tukey's test. Microhardness was analyzed through the Kruskal-Wallis test followed by Dunn's test; the level of significance was 5%. All groups immersed in mouth rinses had a higher level of microhardness loss than CG; additionally, all groups showed changes in the enamel surface. Enamel surface loss was observed using a roughness test, and the mouth rinses promoted a higher level of color changes than CG. Given the results, it can be concluded that the mouth rinses led to significant changes in tooth enamel.

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Section: Color Evaluationmentioning

confidence: 99%

Effect of mouth rinses on tooth enamel surface

Favaro¹,

Guiraldo²,

Lopes³

et al. 2020

J Oral Sci

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“…Indeed, the tunnel preparation approach (even if challenging with regard to caries removal [74]) dispenses with full surgical intervention as the last resort [14], and aims at a complete preservation of the proximal enamel, including the previously demineralized lesion, now reinforced by means of the double-sided infiltration [32]. Although the non-filled polymerized infiltrant resin itself reveals a low microhardness (even after accelerated aging) [37], it is noteworthy that the reported increase of surface microhardness of infiltrated enamel lesions [17][18][19][20][21][22]25] is comparably high; this obviously is due to a uniform complex composed of triethylene glycol dimethacrylate (TEGDMA) and hydroxyapatite, and this interaction with crystals results in improved mechanical strengths [22] and aesthetic appearance [2,23].…”

Section: Discussionmentioning

confidence: 99%

“…Notwithstanding, initial enamel lesions are characterized by a reduced mineral content, thus leading to a decreased microhardness [16], and this in turn will result in a decreased stability, possibly jeopardizing the integrity of the respective regions; resin infiltration, however, has been reported to increase surface microhardness of demineralized human [17][18][19] or bovine [20][21][22] enamel significantly. All in all, this would suggest a recovered surface resistance of the respective areas [23]; nevertheless, it should be kept in mind that in particular with progressed enamel carious lesions the infiltration frequently will be inhomogeneous [24] and incomplete [3,24] with respect to the total lesion depth.…”

Section: Introductionmentioning

confidence: 99%

Ex vivo investigation on internal tunnel approach/internal resin infiltration and external nanosilver-modified resin infiltration of proximal caries exceeding into dentin

Kielbassa¹,

Leimer²,

Hartmann³

et al. 2020

PLoS ONE

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This ex vivo proof-of-concept study aimed to investigate the effect of nanosilver particles (AgNP) added to a conventional infiltrant resin (Icon) on external penetration into natural proximal enamel caries exceeding into dentin after internal tunnel preparation and internal infiltration. Carious lesions (ICDAS codes 2/3) of extracted human (pre-)molars revealing proximal caries radiographically exceeding into dentin (E2/D1 lesions) were preselected. Then, 48 of those specimens showing demineralized areas transcending the enamel-dentin border as assessed by means of near-infrared light transillumination (DIAGNOcam) were deproteinized (NaOCl, 5%). Using an internal tunnel approach, occlusal cavities central to the marginal ridge were prepared. Excavation of carious dentin, total etch procedure (H 3 PO 4 , 40%), and internal resin infiltration (FITC-labeled) followed, along with final restorations (flowable composite resin). Outer lesion surfaces were etched (HCl, 15%) prior to external infiltration (RITC-labeled). Group 1 (control; n = 24) used non-modified infiltrant, while an infiltrant/ AgNP mixture (20 nm; 5.5 wt%) was used with experimental Group 2 (n = 24). Non-infiltrated pores of cut lesions were stained (Berberine), and specimens were analyzed using confocal laser scanning microscopy. Compared to the non-filled infiltrant, incorporation of AgNP had no effect on the resin's external penetration. Between the groups, no significant differences regarding internal or external infiltration could be detected, and non-infiltrated lesion areas did not differ significantly (p>0.109; t-test). The internal tunnel preparation in combination with both an internal resin infiltration and an additional external infiltration approach using a nanosilver-modified infiltrant resin leads to increased infiltrated lesion areas, thus occluding and adhesively stabilizing the porous volume of the demineralized enamel. While exerting antimicrobial effects by the nanosilver particles, this approach should have the potential as a viable treatment alternative for proximal lesions extending into dentin, thus avoiding the sacrifice of sound enamel, postponing the frequently inevitable restoration/re-restoration cycle of conventional proximal caries treatment, and improving dental health.

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“…The initial Vicker's hardness number (VHN) of each enamel surface in a sample was measured on four different points with a micro-indentation hardness tester (Zwick.roell, system 5153. UK) that was fitted with a 50-g load for 15 seconds [10].…”

Section: Methodsmentioning

confidence: 99%

“…More recently, a noninvasive alternative treatment was proposed, based on caries infiltration with a hydrophobic resin. These "infiltrant" materials occlude the micro-pore structures found in de-mineralized enamel and block the passages that bacteria and acid require to cause further dissolution of the enamel structure, thus inhibiting lesion progression [9,10] . Caries infiltration is considered a noninvasive treatment option for noncavitated enamel lesions extending to the outer third of dentin.…”

Section: Introductionmentioning

confidence: 99%

Effect of resin infiltrant and fluoride varnish on micro-hardness of de-mineralized enamel submitted to pH challenge

Attia

2018

Egyptian Dental Journal

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The purpose of this study was to evaluate the micro-hardness of artificially induced demineralized enamel after application of resin infiltrant and fluoride varnish and after pH challenge.Material and methods: In the current study; sound bovine incisors were used. A total of 40 sound enamel were then embedded in pre-cut metal cylinders. Two layers of acid-resistant nail varnish were applied to cover most of the enamel surfaces, leaving a window of 4×4 mm for demineralization. Each specimen was immersed in 32 ml of a de-mineralizing solution containing 50 mM acetate buffer solution and 1.28 mM Ca(NO3)2_4H2O, 0.74 mM (NaH2PO4)_2H2O, and 0.03 ppm F at pH 5.0,10 for 24 hours at 37˚C , then microhardness was recorded. The samples then were divided into two groups according to the material used to treat the de-mineralized enamel, each group consisted of 20 samples. Group 1: The samples of de-mineralized enamel were infiltrated with resin infiltration. Then the micro-hardness was recorded for all resin infiltrated samples before pH cycling challenge. The samples were then submitted to a pH cycling model at 37 C over 7 days. The pH cycling consisted of immersing the samples in 35.5 ml of de-mineralizing solution: (2.0 mmol/ L Ca, 2.0 mmol/ L P, 0.075 mol/ L acetate buffer, 2.22 mL/mm 2 of enamel surface) for 6 hours, alternated with immersion in 17.75 mL of re-mineralizing solution: (1.5 mmol/ L Ca, 0.9 mmol/ L P, 0.15 mol/ L KCl, 0.02 mol/ L cacodylate buffer, pH 7.0, 0.25 mL/mm 2 ) for 18 hours for 5 days. Then, specimens were kept for 2 more days in a fresh re-mineralizing solution, completing 7 days of treatment. The samples were washed in de-ionized water for 30 seconds among demineralizing and re-mineralizing cycles. Group 2: Fluoride varnish (Duraphat , Colgate-Palmolive ,NSW, Australia) was applied as a thin layer by a brush and totally dried, then the micro-hardness was recorded. The samples were then subjected to a pH cycling as in group 1. Then after challenge the micro-hardness measurements were performed as formerly described. Results:The difference was highly significant between resin infiltrant and fluoride varnish treated enamel. The comparison among micro-hardness values of initial, de-mineralized enamel, resin-infiltrated enamel and resin infiltrated after pH cycling showed that there was a significant difference. The difference between micro-hardness values was highly significant when comparison was accomplished between initial and de-mineralized enamel, initial and after pH cycling, demineralized enamel and resin infiltrated enamel, de-mineralized enamel and after pH cycling and finally between resin infiltrated enamel and after pH cycling. The difference between microhardness values was not significant regarding initial and resin infiltrated enamel. Conclusion:Within the limitations of this study, it was concluded that the micro-hardness of resin infiltrated enamel was higher than that of de-mineralized enamel treated with fluoride varnish before and after pH cycling.(500) Reham M. Attia E.D.

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Effect of resin infiltration on the color and microhardness of bleached white‐spot lesions in bovine enamel (an in vitro study)

Abstract: The resin infiltration enhanced the microhardness of the WSLs regardless of whether or not a bleaching treatment was used. Using a bleaching treatment before resin infiltration resulted in color alteration, and it was also found to negatively influence the penetration of the infiltrant.

Cited by 32 publications

References 31 publications

Effect of mouth rinses on tooth enamel surface

Effect of mouth rinses on tooth enamel surface

Ex vivo investigation on internal tunnel approach/internal resin infiltration and external nanosilver-modified resin infiltration of proximal caries exceeding into dentin

Effect of resin infiltrant and fluoride varnish on micro-hardness of de-mineralized enamel submitted to pH challenge

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