Effect of Removing Seminal Plasma Using a Sperm Filter on the Viability of Refrigerated Stallion Semen

Neto, C. Ramires; Monteiro, Gabriel Augusto; Soares, R. F.; Pedrazzi, C.; Dell’Aqua, J.A.; Papa, Federico; Alvarenga, Marco Antônio

doi:10.1016/j.jevs.2012.04.008

Cited by 17 publications

(28 citation statements)

References 16 publications

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“…In a similar study, Ramires Neto et al [19] evaluated the effect of removal of SP by filtration and centrifugation on frozen stallion semen. These authors also found no difference in sperm kinetics (MT, MP, VCL, and RAP) when SP was removed, and they reported semen filtration was as efficient as centrifugation at Â600g for 10 minutes in the maintenance of sperm viability.…”

Section: Discussionmentioning

confidence: 99%

“…The technique generally used for the removal of SP is centrifugation; however, its intensity and duration can negatively interfere with sperm motility and plasma membrane integrity [19]. Another easily applied separation method is the SpermFilter (Ceafepe Tecnologia Veterinária Ind., Sorocaba, São Paulo, Brazil) proposed by Alvarenga et al [20].…”

Section: Introductionmentioning

confidence: 99%

“…This method consists of a synthetic hydrophilic membrane filter with a pore size that permits the passage of SP, while retaining only spermatozoa. In stallions, this filter has been shown to be as efficient as centrifugation in the removal of SP and has the advantage of greater practicality and a lower rate of sperm loss [19].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effect of seminal plasma removal before cryopreservation of bovine semen obtained by electroejaculation on semen quality and in vitro fertility

et al. 2017

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Cryopreservation of bull semen is a common biotechnology procedure in cattle breeding. However, when the ejaculate is obtained by electroejaculation, wide variation is observed in the sperm/seminal plasma (SP) ratio that can affect the freezability of semen in this species. The removal of SP may improve the quality of frozen bull semen. The objective of this study was to evaluate the effect of SP removal from the ejaculate on the cryopreservation of semen from 38 Nellore bulls collected by electroejaculation. After collection, the ejaculate was divided into three aliquots: (1) control (N) diluted to a concentration of 60 × 10 spermatozoa/mL and frozen with SP; (2) centrifugation (C) at ×600g for 10 minutes and the pellet resuspended and frozen at the same concentration as N; and (3) filtration (F) through SpermFilter and sperm recovered and frozen at the same concentration as N. After thawing, sperm kinetics, plasma and acrosome membrane integrity, mitochondrial membrane potential, oxidative stress, and in vitro fertility were evaluated. Statistical analysis was performed using the SAS 9.2 package, and differences were considered significant when P < 0.05. Higher average path velocity and straight-line velocity were observed in the groups submitted to SP removal compared to the control group (P < 0.01). In contrast, filtered samples exhibited higher beat cross frequency, straightness, and linearity compared to the other groups. Plasma membrane integrity was reduced when SP was removed, but lower oxidative stress was observed in groups C and F (34.91 ± 2.95% and 31.63 ± 2.95%, respectively) compared to group N (57.39 ± 2.95%). However, the percentage of hatched blastocysts was similar in the N and F groups (21.22 ± 1.05% and 24.00 ± 1.05%, respectively) and higher compared to group C (18.83 ± 1.05%). In conclusion, removal of SP by centrifugation for bull semen freezing reduced the rate of in vitro-produced embryos, whereas filtration of prefrozen semen was found to be an efficient alternative in terms of semen freezability and in vitro production of bovine embryos.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of seminal plasma removal before cryopreservation of bovine semen obtained by electroejaculation on semen quality and in vitro fertility

et al. 2017

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“…Sperm maturation occurs in the tract of the epididymis, with the sperm acquiring important functions essential for fertilization such as motility, differentiation of the midpiece and acrosome [11] [12] and changes and modifications to the chromatin of the nucleus [5] [13] [31]. The differences detected in the sperm collected in different segments of the epididymis reflect the biochemical changes that occur during sperm passage [32] [33]. When sperm reach the cauda they come in contact with various substances including immobiline which greatly reduces their metabolism to minimize the energy demand and minimize the occurrence of residues; primarily because the epithelium has not been developed for absorbing wastes, which are higher in the segment of the head and somewhat less in the corpus of the epididymis [33] [34].…”

Section: Discussionmentioning

confidence: 99%

Azteca Breed Horse Epididymal Sperm Evaluation: A Comparison of Head, Corpus and Cauda Sperm Quality

Parra-Forero¹,

Mendoza

Góngora³

et al. 2015

ARSci

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The main objective of this study was to determine differences in sperm recovered from the three segments of the epididymis, i.e., head, corpus and cauda, using routine measurements of sperm quality and DNA fragmentation assessment. There was a larger sperm concentration (P < 0.05) in the head (3.34 × 10 6 sperm/mL) compared with the corpus and cauda. The percentage of normal morphology was higher in the cauda but had lower motility (P < 0.05). The DNA fragmentation index was higher for sperm obtained from the head and body of the epididymis (P < 0.01) compared with the cauda. The DNA fragmentation index values were 12% higher (P < 0.01) using Wright stain compared with DAPI stain in sperm from the head and corpus of the epididymis, but similar in the cauda. Spermatobioscopy parameters match the maturation changes that occur in the epididymal tract with higher quality sperm being from the cauda, but with a lower concentration compared to sperm found in the head.

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“…Therefore, the need for an inexpensive, practical, time-efficient method for removal of seminal plasma has driven the development of the SpermFilter (Botu-Pharma, Botucatu, Brazil) (Alvarenga and others 2010, Neto and others 2013). This disposable hydrophilic synthetic membrane filter uses surface tension to draw the smaller molecules of seminal plasma through the filter, leaving the spermatozoa concentrated above the membrane (Alvarenga and others 2010, Neto and others 2013).…”

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confidence: 99%

Comparison of cushioned centrifugation and SpermFilter filtration on longevity and morphology of cooled‐stored equine semen

et al. 2016

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This study compares two methods for seminal plasma removal by evaluating sperm recovery rates, and motility and morphology of cooled-stored semen. Ejaculates were divided into three groups: control, filtration and cushioned centrifugation. Semen was extended to 25 million sperm/ml using a skim-milk-based extender and stored at 5°C for all groups. Sperm motility (total motility (%TM) and progressive motility (%PM)) was determined at 0, 24, 48 and 72 hours by a computer-assisted sperm analyser. Sperm morphology was assessed using differential interference microscopy. Overall, %TM of the centrifugation group was significantly higher than the filter group, but not significantly different than the control. No significant difference in %TM or %PM was detected for the control group and filter. Cushioned centrifugation was a superior method to obtain progressively motile sperm compared with control (P=0.03) and filter groups (P<0.001). No significant difference was found for the per cent of normal sperm cells and detached heads between the groups. This study demonstrated that cushioned centrifugation was a superior method to remove seminal plasma while preserving %TM and enhancing %PM for stallions under cooled storage over three days. However, as the differences appear to be negligible, the SpermFilter may represent an alternative for farms lacking a centrifuge.

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Effect of Removing Seminal Plasma Using a Sperm Filter on the Viability of Refrigerated Stallion Semen

Cited by 17 publications

References 16 publications

Effect of seminal plasma removal before cryopreservation of bovine semen obtained by electroejaculation on semen quality and in vitro fertility

Effect of seminal plasma removal before cryopreservation of bovine semen obtained by electroejaculation on semen quality and in vitro fertility

Azteca Breed Horse Epididymal Sperm Evaluation: A Comparison of Head, Corpus and Cauda Sperm Quality

Comparison of cushioned centrifugation and SpermFilter filtration on longevity and morphology of cooled‐stored equine semen

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