Effect of Removal of the Liver on Blood Coagulation

Shonyo, Elwyn S.; Mann, Frank C.

doi:10.1152/ajplegacy.1950.164.1.111

Cited by 25 publications

(3 citation statements)

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“…Ldecreased; 7 increased; = equal; 0 null. proved by the effects ofremoval of the liver on blood coagulation factors (17), by variation of prothrombin after hepatic injury (18,19), by immunohistochemi~l studies (2) and by synthesis in isolated perfused rat liver (20). Studies of isolated perfused rabbit liver (21) and histologic data (3) demonstrated the role of the liver in the fibrinogen synthesis.…”

Section: Quantitative Datamentioning

confidence: 99%

Albumin, Fibrinogen, Prothrombin and Antithrombin III Variations in Blood, Urines and Liver in Rat Nephrotic Syndrome (Heymann Nephritis)

et al. 1983

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SummaryAlbumin, fibrinogen, prothrombin and antithrombin in (AT ID) variations have been studied in blood, urines and liver during an experimental nephrotic syndrome in rats (Heymann nephritis). A quantitative morphometric study (light microscopy) has been performed in the liver using an immunocytochemical technique – (PAP) method – to evaluate the protein synthesis by the number of protein-containing hepatocytes. Some sections were also studied by electron microscopy. The nephrotic animals were compared with control rats. In the blood of nephrotic rats, fibrinogen and prothrombin concentrations were increased and albumin and AT III concentrations were decreased. In the urines of nephrotic rats, albumin, prothrombin and AT III were lost, but no fibrinogen. The morphometric study in the liver has shown a significantly higher number of fibrinogen and prothrombin containing hepatocytes in nephrotic rats than in controls, suggesting an increased synthesis of these proteins; no change was observed concerning albumin and AT III between nephrotic and control animals. In electron microscopy, albumin was demonstrated in Golgi apparatus, proving that the peroxidase-positive cells are related to protein synthesis. These results show that the mechanisms of regulation of the protein synthesis during nephrotic syndrome are different from one protein to another and, particularly, that their blood level is not the only regulating factor for their synthesis.

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Section: Quantitative Datamentioning

confidence: 99%

Albumin, Fibrinogen, Prothrombin and Antithrombin III Variations in Blood, Urines and Liver in Rat Nephrotic Syndrome (Heymann Nephritis)

et al. 1983

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“…In addition, observations after hepatectomy (3,4) and hepatic injury (5,6) and in hepatic disease (7,8) have provided a body of evidence consistent with hepatic synthesis of fibrinogen, prothrombin, and Factors V, VII, IX, and X. This evidence, however, has not been considered definitive, mainly because it is based on indirect observations in vivo in humans and in animals suffering from variable, often profound, metabolic abnormalities induced by hepatic dysfunction or extirpation.…”

mentioning

confidence: 99%

Synthesis of clotting factors by the isolated perfused rat liver.

Olson¹,

Miller²,

Troup³

1966

J. Clin. Invest.

101

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The liver has been proposed as a site of synthesis of blood-clotting factors, in part because of the demonstrated dominant role of the liver in synthesis of all plasma protein fractions except the y-globulins (1, 2). In addition, observations after hepatectomy (3, 4) and hepatic injury (5, 6) and in hepatic disease (7,8) have provided a body of evidence consistent with hepatic synthesis of fibrinogen, prothrombin, and Factors V, VII, IX, and X. This evidence, however, has not been considered definitive, mainly because it is based on indirect observations in vivo in humans and in animals suffering from variable, often profound, metabolic abnormalities induced by hepatic dysfunction or extirpation.This report describes the use of the isolated perfused rat liver in a more direct experimental evaluation of the role of the liver in the production of blood-clotting factors. The use of the isolated perfused liver was encouraged by Lupton's observations of shortened prothrombin time during perfusions of 3 to 4 hours (9), and by Pool and Robinson's studies of changes in clotting factor activity during in vitro incubation of rat liver slices (10). Presented here is not only more detailed direct evidence of hepatic synthesis of prothrombin and Factor VII, but also the first direct evidence for hepatic synthesis of Factors V and X.

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“…More recently Murphy and Seegers (2) found 190 to 205 units in dog and 290 to 315 in human blood. Mann and his associates (3,4) reported that the average for dog blood is 183 units per cc. and for human blood 319 units.…”

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confidence: 99%