Effect of Reconstituted Pulmonary Surfactant Containing the 6000-Dalton Hydrophobic Protein on Lung Compliance of Prematurely Delivered Rabbit Fetuses

Yu, Shou-Haw; Wallace, Duncan G.; Bhavnani, Bhagu R.; Enhörning, Göran; Harding, Paul G.R.; Possmayer, Fred

doi:10.1203/00006450-198801000-00006

Cited by 28 publications

(14 citation statements)

References 29 publications

(60 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Oxidized SP-B was prepared by treatment of BLES with hypochlorous acid before purification of the SP-B as described via LH-20 chromatography (30,42). Protein levels for purified SP-B and oxidized SP-B were quantified using a modified Lowry procedure (29) containing optimal SDS (30,53); BLES oxidation did not impact protein quantification. All SP-A and SP-B preparations were provided by Drs.…”

Section: Spsmentioning

confidence: 99%

Surfactant protein B inhibits secretory phospholipase A₂hydrolysis of surfactant phospholipids

Hite

Grier

Waite

et al. 2012

American Journal of Physiology-Lung Cellular and Molecular Phys

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Spsmentioning

confidence: 99%

Surfactant protein B inhibits secretory phospholipase A₂hydrolysis of surfactant phospholipids

Hite

Grier

Waite

et al. 2012

American Journal of Physiology-Lung Cellular and Molecular Phys

Self Cite

View full text Add to dashboard Cite

show abstract

“…Estimates of the molecular masses of SP-B and SP-C by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) have given varying results, probably due to heterogeneous preparations, and to the great hydrophobicity of the proteins. Values of 15-18 kDa and about 7 kDa have been obtained for SP-B in nonreduced and reduced form, respectively [207][208][209]. Larger, disulphide-dependent polymers of SP-B, with molecular masses of about 20 and 26 kDa, have also been observed [210].…”

Section: Hydrophobic Proteinsmentioning

confidence: 99%

“…Larger, disulphide-dependent polymers of SP-B, with molecular masses of about 20 and 26 kDa, have also been observed [210]. The corresponding values observed for SP-C are about 10 kDa (nonreduced) and about 5 kDa (reduced) [207][208][209].…”

Section: Hydrophobic Proteinsmentioning

confidence: 99%

“…However, depalmitoylated SP-C and synthetic SP-C without palmitoyl groups are much more difficult to handle than the intact molecule, probably, at least partly, because of formation of oligomeric forms. The disulphide-dependent dimeric forms of SP-C observed upon SDS-PAGE [207][208][209]238], may be artifacts formed by inadvertent cleavage of the thioester linkages and subsequent formation of intermolecular disulphides. However, BAATZ et al [238] reported that about 15-20% of bovine SP-C is probably a true dimer.…”

Section: Functional Correlationsmentioning

confidence: 99%

See 1 more Smart Citation

The proteins of the surfactant system

Johansson¹,

Curstedt²,

Robertson³

1994

Eur Respir J

203

143

View full text Add to dashboard Cite

T Th he e p pr ro ot te ei in ns s o of f t th he e s su ur rf fa ac ct ta an nt t s sy ys st te em m SP-B is a 79-residue polypeptide that contains three intrachain disulphide bridges. It exists mainly as a homodimer, which is strongly positively charged and may selectively remove anionic and unsaturated lipid species from the alveolar surface film, thereby increasing surface pressure.SP-C is a mainly α-helical, extraordinarily hydrophobic polypeptide containing 35 amino acid residues and covalently linked palmitoyl groups. Its α-helical portion is inserted into surfactant lipid bilayers. SP-C accelerates the adsorption of lipid bilayers to an interfacial monolayer. In babies with respiratory distress syndrome, the clinical response to treatment with surfactant containing SP-B and SP-C is much faster than in babies treated with protein-free synthetic surfactant.We speculate that, in the near future, surfactant preparations based on recombinant hydrophobic proteins will be available for clinical use.

show abstract

“…ture (3,4), function (5)(6)(7)(8), and metabolism (9) of surfactant. The role of SP-D in the function of surfactant is not yet defined (10).…”

mentioning

confidence: 99%

Dexamethasone Enhances Surfactant Protein Gene Expression in Streptozotocin-Induced Immature Rat Lungs

1995

View full text Add to dashboard Cite

Because surfactant protein (SP) mRNA levels in rat fetuses are increased by maternal dexamethasone (dex) treatment and decreased in streptozotocin-induced diabetic (STZ-DB) pregnancy, we investigated the in vivo effects of dex on SP gene expression in STZ-DB pregnancy. 2) a 2-3-fold increase in SP-C mRNA levels was observed in response to dex in d 18 and 20 fetuses; 3) the increase in transcription of SP-A and SP-B in d 20 fetuses after dex is 68 and 60%, respectively, of the increase in their mRNA levels whereas in STZ-DB, the decrease in transcription compared with mRNA levels is 3.67-fold for SP-A and 2.42 fold SP-B; and 4) changes in SP-C transcription in either in vivo model, dex-treated or STZ-DB, correspond well with changes in mRNA levels. Together, these findings indicate that dex can enhance SP expression in STZ-DB immature lungs and support differential regulation of fetal SP genes in the models studied. (Pediatr Res 38: 870-877, 1995)

show abstract

Effect of Reconstituted Pulmonary Surfactant Containing the 6000-Dalton Hydrophobic Protein on Lung Compliance of Prematurely Delivered Rabbit Fetuses

Cited by 28 publications

References 29 publications

Surfactant protein B inhibits secretory phospholipase A₂hydrolysis of surfactant phospholipids

Surfactant protein B inhibits secretory phospholipase A₂hydrolysis of surfactant phospholipids

The proteins of the surfactant system

Dexamethasone Enhances Surfactant Protein Gene Expression in Streptozotocin-Induced Immature Rat Lungs

Contact Info

Product

Resources

About

Effect of Reconstituted Pulmonary Surfactant Containing the 6000-Dalton Hydrophobic Protein on Lung Compliance of Prematurely Delivered Rabbit Fetuses

Cited by 28 publications

References 29 publications

Surfactant protein B inhibits secretory phospholipase A2hydrolysis of surfactant phospholipids

Surfactant protein B inhibits secretory phospholipase A2hydrolysis of surfactant phospholipids

The proteins of the surfactant system

Dexamethasone Enhances Surfactant Protein Gene Expression in Streptozotocin-Induced Immature Rat Lungs

Contact Info

Product

Resources

About

Surfactant protein B inhibits secretory phospholipase A₂hydrolysis of surfactant phospholipids

Surfactant protein B inhibits secretory phospholipase A₂hydrolysis of surfactant phospholipids