Effect of Rebamipide, a Novel Antiulcer Agent, on Helicobacter pylori Adhesion to Gastric Epithelial Cells

Abstract

Helicobacter pylori is a major etiological agent in gastroduodenal disorders. The adhesion of H. pylori to human gastric epithelial cells is the initial step of H. pylori infection. Inhibition of H. pylori adhesion is thus a therapeutic target in the prevention of H. pyloriinfection. Experiments were performed to evaluate the effect of rebamipide, a novel antiulcer agent, on H. pylori adhesion to gastric epithelial cells. MKN-28 and MKN-45 cells, derived from human gastric carcinomas, were used as target cells. Ten H. pylori strains isolated from patients with chronic gastritis and gastric ulcer were used in the study. We evaluated the effect of rebamipide on H. pylori adhesion to MKN-28 and MKN-45 cells quantitatively using our previously established enzyme-linked immunosorbent assay. The adhesion of H. pylori to MKN-28 and MKN-45 cells was significantly inhibited by pretreatment of these cells with 100 μg of rebamipide per ml. However, the adhesion was not affected by the pretreatment of H. pylori with rebamipide. On the other hand, the viabilities of H. pylori, MKN-28 cells, and MKN-45 cells were not affected by rebamipide. Our studies suggest that rebamipide inhibits the adhesion of H. pylorito gastric epithelial cells.

Influence of lansoprazole, famotidine, roxatidine and rebamipide administration on the urea breath test for the diagnosis of Helicobacter pylori infection

“…Although the administration of ranitidine at higher doses was demonstrated to decrease UBT sensitivity, 19 our study clarified that the false‐negative rate caused by H2RA administration was far lower than that caused by the administration of a PPI. Rebamipide, which has protective activity on the gastric mucosa, is widely used in patients with gastric ulcers and gastritis in Japan 20,21 . Administration of rebamipide for 14 days did not affect the results of the UBT, probably because rebamipide has no anti‐urease or antisecretory activity 20,21 …”

“…Rebamipide, which has protective activity on the gastric mucosa, is widely used in patients with gastric ulcers and gastritis in Japan 20,21 . Administration of rebamipide for 14 days did not affect the results of the UBT, probably because rebamipide has no anti‐urease or antisecretory activity 20,21 …”

Suppression of CD74 Expression andHelicobacter pyloriAdhesion by Auraptene Targeting Serum Starvation-Activated ERK1/2 in NCI-N87 Gastric Carcinoma Cells

“…The adhesion assay was performed as described previously. 24,25) Briefly, the cells (5:0 Â 10 5 cells/1.0 ml) were grown on a 24-well microplate and preincubated for 24 h. The medium on the plate was then replaced with serum-free RPMI1640 containing the samples dissolved in DMSO for 48 h. The cells were exposed to H. pylori in serum-free RPMI1640 (5:0 Â 10 7 cells/1.0 ml) after the medium had been changed. After 6 h, the medium and unbound H. pylori were washed out three times with phosphatebuffered saline (PBS).…”

15th Anniversary of Rebamipide: Looking Ahead to the New Mechanisms and New Applications

“…There are numerous underlying mechanisms of rebamipide action. It inhibits adhesion of H. pylori to the gastric epithelial cells (34), overexpression of IL-8 caused by H. pylori at the transcriptional level through the inactivation of NF-κB (31), and overexpression of adhesion molecule CD18 on neutrophils caused by this microorganism (35). In addition, ammonia produced by H. pylori is another injurious factor.…”