Effect of rearing temperature on sex ratio in juvenile Atlantic halibut, Hippoglossus hippoglossus

Hughes, Victoria; Benfey, Tillmann J.; Martin-Robichaud, D. J.

doi:10.1007/s10641-007-9214-9

Cited by 12 publications

(3 citation statements)

References 28 publications

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“…This information was obtained mainly from sex ratios in progenies of meiogynogenetics and triploids, and both XX/XY and ZZ/ZW mechanisms have been reported (Purdom 1972;Tabata 1991;Howell et al 1995;Tvedt et al 2006;Chen et al 2009). Environmental factors, such as temperature, do (Tabata 1995;Goto et al 1999;Luckenbach et al 2005) or do not affect (Hughes et al 2008) gonad differentiation in flatfish, but this appears not to be a primary factor in sex determination in this group (OspinaAlvarez and Piferrer 2008). Segregation patterns of the closest markers to the SD region in turbot support a ZZ/ZW mechanism in the five families analyzed.…”

Section: Discussionmentioning

confidence: 99%

Identification of the Major Sex-Determining Region of Turbot (Scophthalmus maximus)

et al. 2009

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Sex determination in fish is a labile character in evolutionary terms. The sex-determining (SD) master gene can differ even between closely related fish species. This group is an interesting model for studying the evolution of the SD region and the gonadal differentiation pathway. The turbot (Scophthalmus maximus) is a flatfish of great commercial value, where a strong sexual dimorphism exists for growth rate. Following a QTL and marker association approach in five families and a natural population, we identified the main SD region of turbot at the proximal end of linkage group (LG) 5, close to the SmaUSC-E30 marker. The refined map of this region suggested that this marker would be 2.6 cM and 1.4 Mb from the putative SD gene. This region appeared mostly undifferentiated between males and females, and no relevant recombination frequency differences were detected between sexes. Comparative genomics of LG5 marker sequences against five model species showed no similarity of this chromosome to the sex chromosomes of medaka, stickleback, and fugu, but suggested a similarity to a sex-associated QTL from Oreochromis spp. The segregation analysis of the closest markers to the SD region demonstrated a ZW/ZZ model of sex determination in turbot. A small proportion of families did not fit perfectly with this model, which suggests that other minor genetic and/or environmental factors are involved in sex determination in this species.

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Section: Discussionmentioning

confidence: 99%

Identification of the Major Sex-Determining Region of Turbot (Scophthalmus maximus)

et al. 2009

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“…Meiotic gynogenetic H. hippoglossus were all-female, suggesting an XX/XY sex-determining system [16]. Temperature has not been shown to have an effect on H. hippoglossus sex ratio [17]. Gonadal sex differentiation can be manipulated through in-feed synthetic steroid treatments ( e.g.…”

Section: Introductionmentioning

confidence: 99%

Mapping the sex determination locus in the Atlantic halibut (Hippoglossus hippoglossus) using RAD sequencing

et al. 2013

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BackgroundAtlantic halibut (Hippoglossus hippoglossus) is a high-value, niche market species for cold-water marine aquaculture. Production of monosex female stocks is desirable in commercial production since females grow faster and mature later than males. Understanding the sex determination mechanism and developing sex-associated markers will shorten the time for the development of monosex female production, thus decreasing the costs of farming.ResultsHalibut juveniles were masculinised with 17 α-methyldihydrotestosterone (MDHT) and grown to maturity. Progeny groups from four treated males were reared and sexed. Two of these groups (n = 26 and 70) consisted of only females, while the other two (n = 30 and 71) contained balanced sex ratios (50% and 48% females respectively). DNA from parents and offspring from the two mixed-sex families were used as a template for Restriction-site Associated DNA (RAD) sequencing. The 648 million raw reads produced 90,105 unique RAD-tags. A linkage map was constructed based on 5703 Single Nucleotide Polymorphism (SNP) markers and 7 microsatellites consisting of 24 linkage groups, which corresponds to the number of chromosome pairs in this species. A major sex determining locus was mapped to linkage group 13 in both families. Assays for 10 SNPs with significant association with phenotypic sex were tested in both population data and in 3 additional families. Using a variety of machine-learning algorithms 97% correct classification could be obtained with the 3% of errors being phenotypic males predicted to be females.ConclusionAltogether our findings support the hypothesis that the Atlantic halibut has an XX/XY sex determination system. Assays are described for sex-associated DNA markers developed from the RAD sequencing analysis to fast track progeny testing and implement monosex female halibut production for an immediate improvement in productivity. These should also help to speed up the inclusion of neomales derived from many families to maintain a larger effective population size and ensure long-term improvement through selective breeding.

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“…The sex of the larvae determined was assessed in 20 specimens per treatment every week, from 110 DPH to 173 DPH. This time chosen was from a previous study of another flatfish, Hippoglossus hippoglossus , which showed that sexual differentiation occurred on 140 DPH (Hughes et al, 2008).…”

Section: Methodsmentioning

confidence: 99%

Exposure of larvae to daily thermocycles affects gonad development, sex ratio, and sexual steroids in Solea senegalensis, kaup

et al. 2011

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The effect of water temperature during the development of fish larvae on sex differentiation is well known, but not so well known is the impact of the daily thermocycles. Our aim was to investigate the effect of early exposure of Senegal sole larvae to different temperature cycles on gonad development, sex ratio, and sex steroid (11-ketotestosterone (11-KT); estradiol (E(2) ); and testosterone, (T)) content in muscle extracts of juveniles. From 1 to 97 days posthatching (DPH) fish larvae and post-larvae were subjected to three temperature regimes: Thermophase-Cryophase (TC), Cryophase-Thermophase (CT), and constant temperature. In fish exposed to TC, sex determination occurred earlier, because 90% of soles were males/females at 110 DPH, whereas 45% of fish under CT were undifferentiated at that time. Fish under TC showed the highest growth rates, followed by fish under constant temperature and by fish under CT, the differences being statistically significant between the TC and CT groups. Regarding sex ratio, juveniles exposed to TC showed a higher proportion of females than fish under CT or constant temperature. Under TC, fish showed the highest concentration of E(2) , whereas 11-KT concentration was highest in fish under CT and constant temperature. Fish under constant temperature and CT showed higher T levels than those under TC. These results provide the first insights into the effect of daily thermocycles on sex differentiation in fish, and underline the key role of natural environmental cycles on the control of sex ratios during larval development, which may be applied to the manipulation of sex ratio in aquaculture.

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Effect of rearing temperature on sex ratio in juvenile Atlantic halibut, Hippoglossus hippoglossus

Cited by 12 publications

References 28 publications

Identification of the Major Sex-Determining Region of Turbot (Scophthalmus maximus)

Identification of the Major Sex-Determining Region of Turbot (Scophthalmus maximus)

Mapping the sex determination locus in the Atlantic halibut (Hippoglossus hippoglossus) using RAD sequencing

Exposure of larvae to daily thermocycles affects gonad development, sex ratio, and sexual steroids in Solea senegalensis, kaup

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