Effect of reactive oxygen species on germination of <i>Cynara cardunculus</i> (L.) cultivars

Huarte, Héctor Roberto; Puglia, G.; Varisco, D; Pappalardo, H.; Calderaro, P.; Toscano, V.; Raccuia, S.A.

doi:10.17660/actahortic.2020.1284.3

Cited by 4 publications

(1 citation statement)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In A. thaliana the addition in the germination medium of methyl viologen, a ROS-generating compound, partially released seed dormancy, while in sunflower, it alleviated significantly dormancy activating downstream elements of the ethylene signaling pathway but without altering ABA production [ 20 , 22 , 23 ]. In wild cardoon, previous studies showed an increment of germination in the presence of H 2 O 2 [ 24 , 25 ]. On the other hand, when ROS level exceeded a certain value, the activation of antioxidant systems was observed in many species, which allows maintaining ROS homeostasis within the oxidative window for germination [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

Seed Transcriptome Annotation Reveals Enhanced Expression of Genes Related to ROS Homeostasis and Ethylene Metabolism at Alternating Temperatures in Wild Cardoon

Huarte

Puglia

Prjibelski

et al. 2020

Plants

View full text Add to dashboard Cite

The association among environmental cues, ethylene response, ABA signaling, and reactive oxygen species (ROS) homeostasis in the process of seed dormancy release is nowadays well-established in many species. Alternating temperatures are recognized as one of the main environmental signals determining dormancy release, but their underlying mechanisms are scarcely known. Dry after-ripened wild cardoon achenes germinated poorly at a constant temperature of 20, 15, or 10 °C, whereas germination was stimulated by 80% at alternating temperatures of 20/10 °C. Using an RNA-Seq approach, we identified 23,640 and annotated 14,078 gene transcripts expressed in dry achenes and achenes exposed to constant or alternating temperatures. Transcriptional patterns identified in dry condition included seed reserve and response to dehydration stress genes (i.e., HSPs, peroxidases, and LEAs). At a constant temperature, we observed an upregulation of ABA biosynthesis genes (i.e., NCED9), ABA-responsive genes (i.e., ABI5 and TAP), as well as other genes previously related to physiological dormancy and inhibition of germination. However, the alternating temperatures were associated with the upregulation of ethylene metabolism (i.e., ACO1, 4, and ACS10) and signaling (i.e., EXPs) genes and ROS homeostasis regulators genes (i.e., RBOH and CAT). Accordingly, the ethylene production was twice as high at alternating than at constant temperatures. The presence in the germination medium of ethylene or ROS synthesis and signaling inhibitors reduced significantly, but not completely, germination at 20/10 °C. Conversely, the presence of methyl viologen and salicylhydroxamic acid (SHAM), a peroxidase inhibitor, partially increased germination at constant temperature. Taken together, the present study provides the first insights into the gene expression patterns and physiological response associated with dormancy release at alternating temperatures in wild cardoon (Cynara cardunculus var. sylvestris).

show abstract

Section: Introductionmentioning

confidence: 99%

Seed Transcriptome Annotation Reveals Enhanced Expression of Genes Related to ROS Homeostasis and Ethylene Metabolism at Alternating Temperatures in Wild Cardoon

Huarte

Puglia

Prjibelski

et al. 2020

Plants

View full text Add to dashboard Cite

show abstract

Effect of Gibberellic Acid on Seed Germination of Onopordum nervosum Subsp. platylepis Murb

Dhen,

Essaidi,

Naffati

et al. 2024

Advances in Science, Technology &Amp; Innovation

View full text Add to dashboard Cite

Reactive oxygen and nitrogen species (RONS) signalling in seed dormancy release, perception of environmental cues, and heat stress response

Puglia

2023

Plant Growth Regul

View full text Add to dashboard Cite

Seed germination is a crucial plant-life process whose success depends largely on the seed's ability to germinate under favourable environmental conditions. Through molecular signalling, a seed is able to perceive environmental information, assimilate it, and transmit signals that determine its destiny. Reactive Oxygen and Nitrogen Species (RONS) function as signalling molecules that influence multiple phases of plant development. In the process of seed germination, their presence generally promotes germination completion, though not to the same extent in all species and environments. As signalling molecules, they participate in the sensing of light and temperature fluctuations as favourable germination cues, but they also play a role in inhibiting germination when temperatures exceed the optimal range, preventing seedling exposure to heat. Depending on environmental conditions, RONS set up crosstalk with the major phytohormones involved in germination, ABA, GA, and even auxin, regulating their biosynthesis and signalling. Here, we show relevant studies on how RONS exert seed germination control on multiple levels, such as through protein oxidation, epigenetic control, promotion of phytohormone key-metabolism genes expression, post-translational protein modifications, and redox interactions with DOG1. This review summarises the current understanding of the role of RONS in the seed, from its maturation to the transduction of environmental conditions. Special consideration is given to the RONS-mediated germination response to favourable stimuli, such as light or temperature fluctuations, and to conditions that inhibit germination, such as high temperatures.

show abstract

Effect of reactive oxygen species on germination of Cynara cardunculus (L.) cultivars

Cited by 4 publications

References 23 publications

Seed Transcriptome Annotation Reveals Enhanced Expression of Genes Related to ROS Homeostasis and Ethylene Metabolism at Alternating Temperatures in Wild Cardoon

Seed Transcriptome Annotation Reveals Enhanced Expression of Genes Related to ROS Homeostasis and Ethylene Metabolism at Alternating Temperatures in Wild Cardoon

Effect of Gibberellic Acid on Seed Germination of Onopordum nervosum Subsp. platylepis Murb

Reactive oxygen and nitrogen species (RONS) signalling in seed dormancy release, perception of environmental cues, and heat stress response

Contact Info

Product

Resources

About