Effect of Rapid Tissue Growth on the Uptake of Fluorene-2,7-Di-(Sulfonamido-2-Naphthalene)-S35 by the Liver and Spleen of Rats and Hamsters

Abstract

PREVIOUS studies on the localization of fluorene-2,7-di-(sulfonamido-2-naphthalene)-S35 revealed that the presence of a subcutaneously transplanted tumor decreased the uptake of this radioactive compound by the liver and spleen of mice (Argus and Hewson, 1954; Argus, Hewson and Ray, 1956). This phenomenon was demonstrated in both CAF1/Jax mice bearing a squamous cell stomach carcinoma and C3H mice bearing the Barrett mammary adenocarcinoma. Autoradiograms indicated that it is a phagocytic function of the reticuloendothelial system that is impaired (Argus, Hewson and Ray, 1956). Subsequent studies to determine if other stress conditions produce this phenomenon showed that, unlike the presence of a tumor, neither X-irradiation nor cortisone affects the uptake of fluorene-2,7-di-(sulfonamido-2-naphthalene)-S35 by the liver and spleen of mice or rats (Argus, Kane and Ray, 1960;Malejka, Argus and Ray, 1961).In the present studies this localization phenomenon was investigated in hamsters and rats as influenced by the presence of two different types of transplanted tumors (a fibrosarcoma and Walker carcinosarcoma 256). The effect of metastatic tumors existing after surgical removal of the transplanted sarcoma was studied in hamsters, and the effects of liver regeneration (resulting from partial hepatectomy) and of pregnancy were observed in rats. To determine if the phenomenon occurs as a result of the increase in proliferation of liver cells (Laird andBarton, 1959, 1960) caused by the carcinogen, 2-acetylaminofluorene, before the gross appearance of tumors, the uptake of fluorene-2,7-di-(sulfonamido-2-naphthalene)-S35 by the liver and spleen of rats fed the carcinogen, 2-acetylaminofluorene, for varying periods of time was investigated. MATERIALS AND METHODSFluorene-2,7-di-(sulfonamido-2-naphthalene)-S35 having a specific activity of 13,400 disintegrations/sec. /mg. (0-36 /ac/mg.) was prepared as previously reported (Argus and Hewson, 1954). For administration, the radioactive compound was dissolved (9 mg./ml.) in 0 05N NaOH except for a study of two groups of hamsters where the injection medium was dilute sodium bicarbonate and * Present address:

Distribution of new fluorene disulphonamido derivatives in rats with transplanted Walker carcinosarcoma.

“…In the spleen of tumour-free rats 1-5 times more Compound VII is found than in the presence of tumour (0.05 < P < 0.1), and also larger amounts of Compounds V and VI are found in this organ of the control group than in the tumour-bearers (the differences between the two groups for Compounds V and VI are not, however, significant). Thus, in general, the four disulphonamides (Compounds IV, V, VI and VII) tend to follow the behaviour of the pattern compound: N,N'-bis(naphthalene)-2,7-fluorene[35S]disulphonamide with which an impaired function of the reticuloendothelial system in the presence of tumour has been shown (Argus, 1961;Argus, Hudson, Seepe, Kane and Ray, 1962).…”

“…The phenomenon of an impaired phagocytic function of the reticuloendothelial system was demonstrated in the presence of various tumours in different species using N,N'-bis(naphthalene)-2, 7-fluorene[35S]disulphonamide (Argus and Hewson, 1954; Argus, Hudson, Seepe, Kane and Ray, 1962). The same reaction was observed with N,N'-bis(p-sulphamoyl-phenyl)-2,7-fluorene[35S]disulphonamide (Malejka, 1962).…”