Effect of Raloxifene on Periapical Lesions in Ovariectomized Rats

Gomes‐Filho, João Eduardo; Wayama, Marcelo Tadahiro; Dornelles, Rita Cássia Menegati; Ervolino, Edílson; Coclete, Gilberto Aparecido; Duarte, Paulo Carvalho Tobias; Yamanri, Guilherme Hiroshi; Lodi, Carolina Simonetti; Dezan, Elói; Cintra, Luciano Tavares Ângelo

doi:10.1016/j.joen.2014.11.027

Cited by 26 publications

(31 citation statements)

References 28 publications

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“…The influence of Lactobacillus rhamnosus and Lactobacillus acidophilus lowered the severity of inflammatory infiltrate and proinflammatory cytokine expression (IL-1b and IL6) concomitantly with higher anti-inflammatory cytokine expression (IL-10). The 30-day experimental period was chosen because it is considered long enough for chronic AP development (Gomes-Filho et al 2015, Cintra et al 2016, Dal-Fabbro et al 2019). Therefore, the null hypothesis was rejected.…”

Section: Discussionmentioning

confidence: 99%

“…Apical periodontitis was induced by exposing the pulp of rat molars to the oral environment allowing pulp tissue infection and culminating in pulp necrosis and subsequent AP development. The 30-day experimental period was chosen because it is considered long enough for chronic AP development (Gomes-Filho et al 2015, Cintra et al 2016, Dal-Fabbro et al 2019). In addition, the chronic AP model can guarantee the absence of signs of acute infection, such as abscesses, which may influence the expression of proinflammatory mediators in periapical tissues.…”

Section: Discussionmentioning

confidence: 99%

“…Histopathological analysis was conducted using the quality of inflammation and the cellularity pattern of dental and periodontal tissues as guidelines to score the inflammatory infiltrate as follows: absent (0 to few inflammatory cells, score = 1), mild (< 25 cells, score = 2), moderate (25-125 cells, score = 3) and severe (> 125 cells, score = 4) (Azuma et al 2017a,b). Presence or absence of necrosis was also recorded (Gomes-Filho et al 2015, Dal-Fabbro et al 2019.…”

Section: Histopathological and Histometric Analysesmentioning

confidence: 99%

“…The measurement was conducted in five equidistant sections to the root canal. For this, an image-processing system was used consisting of a light microscope (DM 4000 B; Leica), a colour camera (DFC 500; Leica), a colour image processor (Leica Qwin V3 software; Leica) and a personal computer (Intel Core i5, Intel Corp, Santa Clara, CA, USA; Windows 10, Microsoft Corp, Redmond, WA, USA) (Gomes-Filho et al 2015).…”

Section: Histopathological and Histometric Analysesmentioning

confidence: 99%

“…Immunolabeling of the histological sections was performed using an indirect immunoperoxidase technique (Gomes-Filho et al 2015. Histological sections were arranged into three batches, and each batch was incubated with one of the following primary antibodies (1:100): IL-10 (Rabbit -orb221323, Biorbyt, San Francisco, CA, USA); IL-1b (Rabbit -orb101745 Biorbyt) and IL 6 (Rabbit -orb6210 Biorbyt).…”

Section: Immunohistochemical Analysismentioning

confidence: 99%

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Systemic administration of probiotics reduces the severity of apical periodontitis

Cosme‐Silva¹,

Dal‐Fabbro²,

Cintra³

et al. 2019

Int Endodontic J

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Aim To evaluate the effect of systemic administration of probiotics on the severity of apical periodontitis (AP). Methodology Twenty‐four male Wistar rats were used. AP was induced in the maxillary left/right first molars. The animals were arranged into groups: Control, Lactobacillus rhamnosus, and Lactobacillus acidophilus. Probiotics were administered orally for gavage (109 colony‐forming units diluted in 5 mL of water for 30 days) during the development of AP. After 30 days, cardiac puncture was performed to analyse the complete blood count. Moreover, microbiological analysis of the root canal contents and saliva was performed. Then, the animals were euthanized and the jaw removed for histopathological and IL‐10, IL‐1β and IL‐6 immunolabeling analyses. After the Shapiro–Wilk test of normality, the Kruskal–Wallis followed by Dunn's test was performed for nonparametric data, and analysis of variance followed by the Tukey test was performed for parametric data (P < 0.05). Results No significance difference was observed in the blood profiles and in the counts of microorganisms from the saliva samples among the groups (P > 0.05). Total microorganism counts in the root canal, the inflammatory infiltrate and the immunostaining for IL‐1β and IL‐6 in AP were significantly lower in the probiotic groups when compared with the control group (P < 0.05). IL‐10 was significantly more immunolabled in the probiotic groups than in the control group (P < 0.05). Conclusion Supplementation with probiotics (Lactobacillus rhamnosus and Lactobacillus acidophilus) had a significant effect on the severity of apical periodontitis in rats, demonstrating the anti‐inflammatory effect of probiotics on the development of apical periodontitis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Histopathological and Histometric Analysesmentioning

confidence: 99%

Section: Histopathological and Histometric Analysesmentioning

confidence: 99%

Section: Immunohistochemical Analysismentioning

confidence: 99%

See 3 more Smart Citations

Systemic administration of probiotics reduces the severity of apical periodontitis

Cosme‐Silva¹,

Dal‐Fabbro²,

Cintra³

et al. 2019

Int Endodontic J

View full text Add to dashboard Cite

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Experimental apical periodontitis alters salivary biochemical composition and induces local redox state disturbances in the salivary glands of male rats

Vazão,

Claudino,

Pimpinato

et al. 2024

Clin Oral Invest

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Evolution of endodontic medicine: a critical narrative review of the interrelationship between endodontics and systemic pathological conditions

et al. 2021

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Endodontics has gained emphasis in the scientific community in recent years due to the increase in clinical and in animal models studies focused on endodontic medicine, which aims to evaluate the interrelationship between systemic and periapical tissues pathological conditions. These studies have shown that systemic changes can boost the pathogenesis of endodontic infection, favoring its development and progression. A contrary relationship is reported in numerous studies that affirm the potential of endodontic infection to trigger systemic damage and may lead to the worsening of pre-existing pathologies. Recently, the potential of filling materials to develop systemic changes such as neurological alterations had been evaluated, also showing that systemic diseases can negatively influence tissue responses to filling materials after endodontic treatment. Despite advances in endodontic medicine studies, there are still gaps in knowledge on the mechanisms of interactions between apical periodontitis (AP) and systemic diseases and much research to be done. In this sense, this critical narrative literature review aimed to show the evolution of studies in endodontic medicine to help the endodontist to know the role of systemic diseases in the pathogenesis of AP and the possible interference in the repair of periapical tissues after endodontic treatment, as well as to evidence the systemic complications that can be triggered or aggravated in the presence of endodontic infection.

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Effect of Raloxifene on Periapical Lesions in Ovariectomized Rats

Cited by 26 publications

References 28 publications

Systemic administration of probiotics reduces the severity of apical periodontitis

Systemic administration of probiotics reduces the severity of apical periodontitis

Experimental apical periodontitis alters salivary biochemical composition and induces local redox state disturbances in the salivary glands of male rats

Evolution of endodontic medicine: a critical narrative review of the interrelationship between endodontics and systemic pathological conditions

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