Effect of Radiation and Chemical Mutagen on Variability in Saffron (Crocus Sativus L.)

The results of the study of 12 varieties and promising lines of glumaceous oat in the competitive variety testing of the FASC of the North-East (Kirov region) in the period from 2018 to 2020 are presented. The purpose of the research is to assess the productivity, ecological plasticity and stability of the genotypes of glumaceous oat for feed and universal use in the conditions of the Volga-Vyatka region. The dependences of grain yield and dry matter harvesting on the state of agro-climatic resources (HTC), temperature and precipitation are established. The contrasting weather conditions during the years of research allowed to conduct the most complete assessment of the studied genotypes. The most favorable conditions for the formation of a high yield of green mass of oat were formed in 2020 (Ij=3.76), grain - in 2019 (Ij=1.35). The average degree of positive dependence of green mass yield on the height of oat plants was revealed (r=0.51). The variety of the high-intensity type of the universal direction Medved, promising lines of the mowing direction (178h13 and 245h14) are distinguished.

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“…During this period, the need of plants for heat and moisture increases [17]. The yield is significantly affected by environmental factors [3,18].…”

Section: Resultsmentioning

confidence: 99%

Promising varieties of spring glumaceous oat of the mowing and grain-mowing directions, adapted to the natural and climatic conditions of the Volga-Vyatka region

Vologzhanina

Баталова

2021

BIO Web Conf.

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“…To our knowledge, in vitro EMS mutagenesis in saffron has received no attention so far. Past scientific reports on mutation induction in saffron have been entirely ex-vitro, using physical and chemical mutagens (Nehvi et al 2009;Khan et al 2011;Salwee and Nehvi 2014). Among the treatment methods tested, the dip method was successful in terms of various growth responses following mutagenic treatment, whereas the inoculation (media enrichment) and direct injection method failed to produce any response.…”

Section: Ideal Treatment Methods and Optimum Ems Dosagementioning

confidence: 99%

“…The autotriploid nature of C. sativus renders the prospects of crop improvement through traditional means, like hybridization, very unlikely (Basker and Negbi 1983). However, there have been efforts by different researchers in this direction using non-conventional breeding techniques such as biotechnological interventions and use of mutagens for induction of genetic variability in saffron (Dhar et al 1988;Bagheri 1989;Nehvi 2003;Nehvi et al 2010;Khan et al 2011). Mutation breeding is an important tool in the improvement of vegetatively propagated crops; particularly in plants with reproductive sterility, where this is the only alternative (Broertjes and Van Harten 2013).…”

Section: Introductionmentioning

confidence: 99%

Development of an efficient in vitro mutagenesis protocol for genetic improvement of saffron (Crocus sativus L.)

Kashtwari

Wani

Dhar

et al. 2018

Physiol Mol Biol Plants

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Saffron ( L) is a triploid (2n = 3x = 24), sterile geophyte which can only be propagated by means of underground vegetative corms. Since corm multiplication does not induce genome variations, therefore, the entire saffron population is expected to have a similar genetic makeup. Keeping in view the economic importance of the plant and the factors responsible for its low yield, the present investigation has been undertaken to establish an in vitro ethyl methanesulfonate (EMS) mutagenesis protocol followed by characterization of the induced variability in the advanced generations. The present report is limited to standardization of in vitro mutagenesis protocol only. Among the mutagenic treatments tested, concentrations ranging from 0.1 to 0.5% EMS showed a varied survival of explants. Based on various growth parameters, the LD was calculated to be 0.3% EMS for 3 h. Among the two types of explants analyzed, the corm explant gave better results for in vitro survival and the growth parameters than callus explant. An average of 57.33 and 92.00 daughter cormlets in all EMS treatments as compared to 47.67 and 57.67 daughter cormlets in control, obtained from callus and corm explants respectively, were transferred to the field. The maximum, average daughter cormlet weight was obtained in control (3.01 g, corm explant) followed by 0.1% EMS (2.8 g, corm explant). In general, the growth parameters showed decreasing trend with an increase in EMS concentration in both the explants. The present study has been a significant achievement in the sense that the first mutagenesis protocol for has been standardized.

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“…In vitro mutagenesis using EMS in saffron has not yet received attention. Previous scientific reports on the mutation induction were entirely in vivo using physical and chemical mutagens (Nehvi et al, 2010;Khan et al, 2011;Salwee and Nehvi, 2014). It is widely emphasized that EMS can induce nuclear as well as a cytoplasmic mutation in cultivated plants (Akhund et al, 1975).…”

Section: Induced Mutagenesis At Crocus Sativusmentioning

confidence: 99%

Micropropagation and potential of bioactive compounds of saffron (Crocus sativus L.) for nutrition and health

et al. 2022

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To inquire about the possibility of durable valorization of saffron, this review highlights the different techniques of in vitro culture and varietal creation of this specie. Then, a reveal main component of saffron with some pharmacological activities that make this product a serious therapeutic hope. Saffron (Crocus sativus L.) is a sterile triploid geophyte that propagated by corms. To respond to the increasing global demand for saffron, it is necessary to expand the area under cultivation. Thus, in vitro techniques can produce a large quantity of propagating material in reduced time. It is well-known that saffron is traditionally used as a coloring or flavoring agent, but recent research showed its potential for health promotion. The interest components include crocin, crocetin, picrocrocin, and safranal have all demonstrated a wide range of use in medical field. Previous studies have reported that biological activities of saffron alleviates or prevents health problems such as stomach upset, cardiovascular disease, and depression. In addition, saffron is also promising in cancer prevention due to its antioxidant properties.

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Effect of Radiation and Chemical Mutagen on Variability in Saffron (Crocus Sativus L.)

Cited by 12 publications

References 5 publications

Promising varieties of spring glumaceous oat of the mowing and grain-mowing directions, adapted to the natural and climatic conditions of the Volga-Vyatka region

Promising varieties of spring glumaceous oat of the mowing and grain-mowing directions, adapted to the natural and climatic conditions of the Volga-Vyatka region

Development of an efficient in vitro mutagenesis protocol for genetic improvement of saffron (Crocus sativus L.)

Micropropagation and potential of bioactive compounds of saffron (Crocus sativus L.) for nutrition and health

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