Effect of Rabbit Spermatozoa on the Incorporation of Labeled Precursors into Endometrial Macromolecules

3H-sialyl residues transfer by subcellular epididymal fractions [plasma membrane (PM), microsomes (MI), and mitochondria (MT)] to both endogenous and exogenous acceptors was studied. Their fraction purity was valued (S'nucleotidase and glucose 6-phosphatase activities). The glycoprotein or glycolipid N-acetyl neuraminyl transferase activity in microsomes were 5 times higher in caput than in cauda (624 to 125 pmoles/30 min/mg protein). PM activity also was higher in caput. In MT fraction, the activity was smaller.Two mechanisms related to spermatozoa glycoprotein changes during maturation are proposed: secretion to the lumen of molecules previously glycosilated in microsomes and transialylation to spermatozoa from membranal ectoenzymes localized on the surface of the epididymal epithelium.

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Re-Evaluation of the Role of Spermatozoa as Inducers of Protein Synthesis by the Rabbit Endometrium

Hernández-Pérez

Luna

Reyes

1983

Archives of Andrology

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The effect of spermatozoa as inducers of protein synthesis by the rabbit endometrium was studied. The presence of spermatozoa increased the leucine incorporation into proteins from 15.6 +/- 1.8 to 44.8 +/- 4.3 dpm X 10(-3)/mg DNA after 2 hr of incubation. There was no difference in the amount of incorporated leucine induced by live or by dead spermatozoa. Latex particles also induced an increase on protein synthesis (24 +/- 3.1 dpm X 10(-3)/mg DNA). These results seem to indicate that this increase in protein synthesis was nonspecific. The number of contaminant cells was always significantly greater in the recovered incubation medium after incubation with highly metabolically active sperm than with dead cells (230 +/- 85 and 86 +/- 33 X 10(-3) cells respectively). This leukocytic effect was smaller than that found in In vivo systems and may play a significant role on the proposed activity of sperm cells on the endometrium.

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Effect of Rabbit Spermatozoa on the Incorporation of Labeled Precursors into Endometrial Macromolecules

Cited by 3 publications

References 7 publications

Induction of antioxidant enzymes by dexamethasone in the adult rat lung

Induction of antioxidant enzymes by dexamethasone in the adult rat lung

Microsomal and Plasma Membrane Sialyltransferase Activity in Rat Epididymis

Re-Evaluation of the Role of Spermatozoa as Inducers of Protein Synthesis by the Rabbit Endometrium

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