Effect of quercetin on nonshivering thermogenesis of brown adipose tissue in high-fat diet-induced obese mice

Pei, Ya; Otieno, Dammah; Gu, Inah; Lee, Sun‐Ok; Parks, John S.; Schimmel, Keith; Kang, Hye Won

doi:10.1016/j.jnutbio.2020.108532

Cited by 45 publications

(26 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our results showed that the combination of quercetin and vitamin E reduced the total cholesterol levels and increased the triglyceride levels in aged male chickens. This result was consistent with the findings of Pei et al (2021) , Jeong et al ( 2012 , 2005 ), and Hidiroglou et al (2004) who reported that Quercetin and vitamin E played a vital role in reducing cholesterol levels in animals. Additionally, the liver and reproductive systems interact in a multifaceted bidirectional manner.…”

Section: Discussionsupporting

confidence: 93%

Dietary quercetin and vitamin E supplementation modulates the reproductive performance and antioxidant capacity of aged male breeder chickens

et al. 2022

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 93%

Dietary quercetin and vitamin E supplementation modulates the reproductive performance and antioxidant capacity of aged male breeder chickens

et al. 2022

View full text Add to dashboard Cite

“…This is in agreement with previous studies demonstrating that high-fat diets are linked to increased oxidative stress, leading to mitochondrial dysfunction and additional ROS production [86,87]. Oxidative stress induced by high-fat diets has been shown to be blocked by dietary factors, such as yogurt, quercetin, geraniin (polyphenol derived from Nephelium lappaceum L. fruit rind), and Terminalia arjuna extract, to name a few [47][48][49][50]. Indeed, in our study, worms fed HB101 or HT115 following MeHg exposure had significantly lower ROS production than the worms fed OP50.…”

Section: Discussionsupporting

confidence: 91%

“…Oxidative stress is a hallmark of MeHg exposure and can drive neurotoxicity and metabolic alterations. Dietary components can quench ROS [47][48][49][50]. We therefore investigated whether altering the bacterial diet fed to worms could prevent oxidative stress derived from MeHg treatment in C. elegans.…”

Section: Bacterial Diet Improves Measures Of Oxidative Stress In Response To Mehgmentioning

confidence: 99%

Methylmercury-Induced Metabolic Alterations in Caenorhabditis elegans Are Diet-Dependent

Crawford

Martell

Nielsen

et al. 2021

Toxics

View full text Add to dashboard Cite

Methylmercury (MeHg) is a well-known neurotoxicant; however, its role in metabolic diseases has been gaining wider attention. Chronic exposure to MeHg in human populations shows an association with diabetes mellitus and metabolic syndrome (MS). As the incidences of both obesity and MS are on the rise globally, it is important to understand the potential role of MeHg in the development of the disease. There is a dearth of information on dietary interactions between MeHg and lipids, which play an important role in developing MS. We have previously shown that MeHg increases food seeking behaviors, lipid levels, fat storage, and pro-adipogenic gene expression in C. elegans fed the standard OP50 Escherichia coli diet. However, we hypothesized that these metabolic changes could be prevented if the worms were fed a bacterial diet lower in lipid content. We tested whether C. elegans developed metabolic alterations in response to MeHg if they were fed two alternative E. coli strains (HT115 and HB101) that are known absorb significantly less lipids from their media. Additionally, to explore the effect of a high-lipid and high-cholesterol diet on MeHg-induced metabolic dysfunction, we supplemented the OP50 strain with twice the standard concentration of cholesterol in the nematode growth media. Wild-type worms fed either the HB101 or HT115 diet were more resistant to MeHg than the worms fed the OP50 diet, showing a significant right-hand shift in the dose–response survival curve. Worms fed the OP50 diet supplemented with cholesterol were more sensitive to MeHg, showing a significant left-hand shift in the dose–response survival curve. Changes in sensitivity to MeHg by differential diet were not due to altered MeHg intake in the worms as measured by inductively coupled mass spectrometry. Worms fed the low-fat diets showed protection from MeHg-induced metabolic changes, including decreased food consumption, lower triglyceride content, and lower fat storage than the worms fed either of the higher-fat diets. Oxidative stress is a common characteristic of both MeHg exposure and high-fat diets. Worms fed either OP50 or OP50 supplemented with cholesterol and treated with MeHg had significantly higher levels of reactive oxygen species, carbonylated proteins, and loss of glutathione than the worms fed the HT115 or HB101 low-lipid diets. Taken together, our data suggest a synergistic effect of MeHg and dietary lipid levels on MeHg toxicity and fat metabolism in C. elegans, which may affect the ability of MeHg to cause metabolic dysfunction.

show abstract

“…Previous studies indicated that EMIQ downregulates adipocyte differentiation and, simultaneously upregulates fatty acid oxidation and WAT browning through the activation of AMP-activated protein kinase (AMPK) α in vivo [ 30 ]. Another study suggested that quercetin increases WAT browning and BAT nonshivering thermogenesis by upregulating β3-AR, mitogen-activated protein kinase (MAPK), and AMPK pathways in an HFD-induced obesity model in mouse [ 39 ]. Further investigation is required to understand the molecular mechanisms underlying the anti-obesity effects of HTGT and EMIQ.…”

Section: Discussionmentioning

confidence: 99%

Anti-obesity effects of heat-transformed green tea extract through the activation of adipose tissue thermogenesis

Lee

Kim

et al. 2022

Nutr Metab (Lond)

View full text Add to dashboard Cite

Background Adipose tissue thermogenesis is a potential therapeutic target to increase energy expenditure and thereby combat obesity. The aim of the present study was to investigate the thermogenic and anti-obesity effects of heat-transformed green tea extract (HTGT) and enzymatically modified isoquercetin (EMIQ). Methods Immortalized brown pre-adipocytes and C3H10T1/2 cells were used for in vitro analyses. A high-fat diet (HFD)-induced obesity mouse model and CIDEA-reporter mice were used for in vivo experiments. The effects of HTGT and EMIQ on mitochondrial metabolism were evaluated by immunoblot, mitochondrial staining, and oxygen consumption rate analyses. In vivo anti-obesity effects of HTGT and EMIQ were measured using indirect calorimetry, body composition analyses, glucose tolerance tests, and histochemical analyses. Results Co-treatment with HTGT and EMIQ (50 μg/mL each) for 48 h increased brown adipocyte marker and mitochondrial protein levels (UCP1 and COXIV) in brown adipocytes by 2.9-fold, while the maximal and basal oxygen consumption rates increased by 1.57- and 1.39-fold, respectively. Consistently, HTGT and EMIQ treatment increased the fluorescence intensity of mitochondrial staining in C3H10T1/2 adipocytes by 1.68-fold. The combination of HTGT and EMIQ (100 mg/kg each) increased the expression levels of brown adipocyte markers and mitochondrial proteins in adipose tissue. Two weeks of HTGT and EMIQ treatment (100 mg/kg each) led to a loss of 3% body weight and 7.09% of body fat. Furthermore, the treatment increased energy expenditure by 8.95% and improved glucose tolerance in HFD-fed mice. Conclusions The current study demonstrated that HTGT and EMIQ have in vivo anti-obesity effects partly by increasing mitochondrial metabolism in adipocytes. Our findings suggest that a combination of HTGT and EMIQ is a promising therapeutic agent for the treatment of obesity and related metabolic diseases.

show abstract

Effect of quercetin on nonshivering thermogenesis of brown adipose tissue in high-fat diet-induced obese mice

Cited by 45 publications

References 45 publications

Dietary quercetin and vitamin E supplementation modulates the reproductive performance and antioxidant capacity of aged male breeder chickens

Dietary quercetin and vitamin E supplementation modulates the reproductive performance and antioxidant capacity of aged male breeder chickens

Methylmercury-Induced Metabolic Alterations in Caenorhabditis elegans Are Diet-Dependent

Anti-obesity effects of heat-transformed green tea extract through the activation of adipose tissue thermogenesis

Contact Info

Product

Resources

About