Effect of Quercetin-loaded liposomes on induced oxidative stress in human spermatozoa

Moretti, Elena; Mazzi, Lucia; Bonechi, Claudia; Salvatici, Maria Cristina; Iacoponi, Francesca; Rossi, Claudio; Collodel, Giulia

doi:10.1016/j.reprotox.2016.02.012

Cited by 30 publications

(24 citation statements)

References 50 publications

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“…The results of this study demonstrated that the C. sativa leaf extract used at dilutions of 1 : 100, 1 : 200, and 1 : 500 did not show any toxicity on sperm motility. These results are different from those obtained by some of our group using purified polyphenols [20,21], which, at high concentration, decreased sperm motility and viability and damaged DNA, as we observed using liposomes loaded with 100 μM quercetin [42].…”

Section: Oxidative Medicine and Cellular Longevitycontrasting

confidence: 99%

“…We have previously demonstrated the scavenging activity of some natural compounds, such as resveratrol, quercetin, rutin, naringenin, epicatechin, and Propolfenol®, a phytocomplex rich in European propolis and catechins, against LPO induced in vitro to human spermatozoa [20][21][22]42]. Recently, Kedechi et al [39] demonstrated the antioxidant Oxidative Medicine and Cellular Longevity effect of hydroxytyrosol, the main phenolic compound of virgin oil, during sperm centrifugation.…”

Section: Oxidative Medicine and Cellular Longevitymentioning

confidence: 99%

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Antioxidant Effect of the Castanea sativa Mill. Leaf Extract on Oxidative Stress Induced upon Human Spermatozoa

Biagi

Noto

Corsini

et al. 2019

Oxidative Medicine and Cellular Longevity

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This study was aimed at evaluating in vitro the effects of a 75% v/v ethanolic extract of leaves of Castanea sativa Mill. (var. Bastarda Rossa, Mount Amiata, Tuscany, Italy) on ejaculated human sperm. Total polyphenols and total flavonoids contained in the extract were determined by a colorimetric assay and HPLC-DAD. The DPPH test and electrochemistry were utilized to study the antioxidant activity of the extract. Swim-up-selected sperm from 8 healthy men were treated with the C. sativa leaf extract at different dilutions (1 : 100, 1 : 200, and 1 : 500), and sperm motility was assessed following the WHO guidelines. Swim-up-selected spermatozoa were incubated with 100 μM H2O2 to induce lipid peroxidation (LPO) and with H2O2 and the leaf extract (1 : 100, 1 : 200, and 1 : 500) to test the antioxidant activity of the extract. The levels of LPO were determined by measuring malondialdehyde (MDA) concentrations. The treated samples were also analyzed by transmission electron microscopy (TEM) for ultrastructural evaluation. The chemical analysis showed that one-third ca. of the polyphenols in the C. sativa extract were made up of flavonoids, with hyperoside present in high concentration. A good antioxidant activity was demonstrated by both the DPPH test and electrochemical analysis. The C. sativa leaf extract did not decrease sperm motility at all tested dilutions. Treatment with H2O2 alone caused a significant increment in MDA levels (P=0.006993), while the treatment with H2O2 plus C. sativa extract diluted to 1 : 100 and 1 : 200 significantly reduced MDA levels (P=0.01476 and P=0.01571, respectively), with respect to H2O2 alone. TEM analysis confirmed the protective effect of the extract on damage induced by LPO, in particular that occurring at the plasma membrane level. The C. sativa leaf extract could be used in human and farm animal protocols for gamete handling, such as techniques of assisted reproduction and cryopreservation of semen, all conditions in which oxidative stress is exacerbated.

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Section: Oxidative Medicine and Cellular Longevitycontrasting

confidence: 99%

Section: Oxidative Medicine and Cellular Longevitymentioning

confidence: 99%

Antioxidant Effect of the Castanea sativa Mill. Leaf Extract on Oxidative Stress Induced upon Human Spermatozoa

Biagi

Noto

Corsini

et al. 2019

Oxidative Medicine and Cellular Longevity

Self Cite

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“…The AO (3, 6‐bis [dimethylamino] acridine, hemi [zinc chloride] salt, BDH Chemicals Ltd, Poole, England) test was performed as previously reported by Tejada et al. () and Moretti et al., . Slides were evaluated with a Leitz Aristoplan Fluorescence Microscope (Leica, Wetzlar, Germany) equipped with a 490‐nm excitation light and a 530‐nm barrier filter.…”

Section: Methodsmentioning

confidence: 99%

Sperm with fibrous sheath dysplasia and anomalies in head-neck junction: focus on centriole and centrin 1

et al. 2016

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Spermatozoa with a rare combination of two monomorphic sperm defects, dysplasia of the fibrous sheath (DFS) and alterations in head-mid-piece junction were analysed. The main focus was to explore the status of the centriole, a key organisation during fertilisation, using the centrin 1, a calcium-binding protein linked to this structure. The sperm quality was examined by light, scanning and transmission electron microscopy (SEM, TEM); immunocytochemistry was performed for tubulin, A-kinase anchor protein 4 (AKAP4) and centrin 1. Spermatozoa showed DFS defect associated with anomalies in head-tail attachment detected by SEM and TEM. Immunolocalisation of tubulin, AKAP4 and centrin 1 confirmed these alterations. Centrin 1 was visible in 67% of spermatozoa (in only 13% centrin localised in a normal position); in the majority of sperm centrin 1's location was altered, sometimes bent; often four spots, indicating the presence of two implantation fossae, were detected. At the centriolar level, immunoreactive fragments, frequently invading the entire short and thick tail, were observed. Centrin 1 is an essential component of the spermatozoa connecting piece and plays a role in centrosome dynamics during sperm morphogenesis and in zygotes and early embryos during spindle assembly. It is important to shed light on these rare conditions in order to better manage the patients during assisted reproductive technology.

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“…Ancak, hem anti-kanser etkisini ortaya çıkarmak, hem de hedef dokuya daha kolay ulaşmasını sağlamak için yeni yaklaşımlara gereksinim vardır. Kuersetin nanopartikülleri ile yapılan çalışmalar kullanılan dozun düşürülmesi, biyoyararlanımının artması ve anti-kanser etkisinde artış gibi sonuçlara neden olmuştur (88,89). Ayrıca, kuersetin türevlerinin sentezi ile hem toksisitenin azaltılacağı hem de biyoyararlanımının ve etkinliğinin arttırılabileceği savunulmaktadır (90,91).…”

Section: şEkil 5 Kuersetinin Anti-kanser Etkisiyle Ilgili Hücre Içi unclassified

Kurkumi̇n, Kuerseti̇n Ve Çay Kateşi̇nleri̇ni̇n Anti̇-Kanser Etki̇leri̇

Yalçın¹,

Yılmaz²,

Altundağ³

et al. 2016

Marmara Pharmaceutical Journal

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Effect of Quercetin-loaded liposomes on induced oxidative stress in human spermatozoa

Cited by 30 publications

References 50 publications

Antioxidant Effect of the Castanea sativa Mill. Leaf Extract on Oxidative Stress Induced upon Human Spermatozoa

Antioxidant Effect of the Castanea sativa Mill. Leaf Extract on Oxidative Stress Induced upon Human Spermatozoa

Sperm with fibrous sheath dysplasia and anomalies in head-neck junction: focus on centriole and centrin 1

Kurkumi̇n, Kuerseti̇n Ve Çay Kateşi̇nleri̇ni̇n Anti̇-Kanser Etki̇leri̇

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