Effect of Qing’e formula on the in vitro differentiation of bone marrow-derived mesenchymal stem cells from proximal femurs of postmenopausal osteoporotic mice

Shuai, Bo; Shen, Lin; Zhu, Rui; Zhou, Ping

doi:10.1186/s12906-015-0777-2

Cited by 19 publications

(14 citation statements)

References 31 publications

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“…It is widely recognized that AR activating the neurotransmitter-based effects [24–27], rather than the estrogen activity [28, 29], is an important mechanism for alleviating menopausal symptoms, such as hot flashes and night sweats. Numerous studies have also confirmed that AR does not have a stimulating effect on the levels of blood estrogen and progestin or estrogen target organs such as the uterus, ovaries, breast, and blood vessels [9, 22, 30]. However, studies on the effect of AR on the skeletal system revealed that AR promotes the secretion of OPG and OC by human osteoblasts, increases the OPG/RANKL ratio, and promotes the formation of mineralized nodules in MC3T3-E1 preosteoblasts, all of which could be blocked by ICI 182,780 [17, 18].…”

Section: Discussionmentioning

confidence: 99%

“…A previously described method was applied for cell extraction and maintenance [22]. SD rats were euthanized and the thigh and shank bones were quickly isolated.…”

Section: Methodsmentioning

confidence: 99%

“…Cells were collected on day 8 after treatment, lysed as previously described, and centrifuged [22]. The supernatant (30 μ L) was evenly mixed with detection reagent, and color development was measured as absorbance at 520 nm using a microplate reader.…”

Section: Methodsmentioning

confidence: 99%

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Role of the ER/NO/cGMP Signaling Pathway in the Promotion of Osteogenic Differentiation of Rat Bone Marrow Mesenchymal Stem Cells by Actaea racemosa Extract

Yang

Zhou²,

Shuai

et al. 2016

Evidence-Based Complementary and Alternative Medicine

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Purpose/Objective. To investigate the effect of Actaea racemosa (AR) extract on in vitro osteogenic differentiation of rat bone marrow mesenchymal stem cells (BMSCs) via the ER/NO/cGMP signaling pathway. Methods/Materials. Rat BMSCs were treated with osteogenic differentiation-inducing medium containing AR; estrogen receptor antagonist, ICI 182,780 (10−6 mol/L); and nitric oxide synthase inhibitor, L-nitro arginine methyl ester (L-NAME, 6 × 10−3 mol/L). Markers of osteogenic differentiation (alkaline phosphatase [ALP] activity, osteocalcin secretion, and calcium ion deposit levels) and the levels of key signaling molecules (nitric oxide synthase [NOS], nitric oxide [NO], and cyclic guanosine monophosphate [cGMP]) were assessed. Results. AR (10−1–10−6 g/L) increased ALP activity in a dose-dependent manner, and the highest ALP, osteocalcin, and osteoprotegerin activities were achieved at an AR concentration of 10−4 g/L. Therefore, the concentration of 10−4 g/L was used for promoting osteogenic differentiation of BMSCs in subsequent analyses. At this concentration, AR increased the levels of NO and cGMP, and such effects could be blocked by the estrogen receptor antagonist (ICI 182,780) and nitric oxide synthase inhibitor (L-NAME). Conclusion. AR induced osteogenic differentiation of rat BMSCs through the ER/NO/cGMP signaling pathway. This finding provides the theoretical foundation for the mechanism of AR in the treatment of postmenopausal osteoporosis.

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Section: Discussionmentioning

confidence: 99%

“…A previously described method was applied for cell extraction and maintenance [22]. SD rats were euthanized and the thigh and shank bones were quickly isolated.…”

Section: Methodsmentioning

confidence: 99%

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Role of the ER/NO/cGMP Signaling Pathway in the Promotion of Osteogenic Differentiation of Rat Bone Marrow Mesenchymal Stem Cells by Actaea racemosa Extract

Yang

Zhou²,

Shuai

et al. 2016

Evidence-Based Complementary and Alternative Medicine

Self Cite

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“…Therefore, understanding the time and dose effects of ZGP on BMSC osteogenic differentiation will be clinically relevant in the treatment of OP. Although the effect of classic kidney-tonifying compounds on BMSC osteogenic differentiation is still poorly understood, a few preparations such as six-ingredient rehmannia pill, sexoton pill [ 71 , 72 ], Erxian decoction [ 73 ], and Qing'e pill [ 74 ] have been shown to promote osteogenic differentiation and inhibit angiogenic differentiation of BMSCs. Therefore, it is important to compare the effects of the different kidney-tonifying compounds on BMSC osteogenic differentiation vis-à-vis OP treatment.…”

Section: Future Directions?mentioning

confidence: 99%

Mechanisms of Zuogui Pill in Treating Osteoporosis: Perspective from Bone Marrow Mesenchymal Stem Cells

Yang

You

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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The current treatment strategies for osteoporosis (OP) involve promoting osteogenic differentiation and inhibiting adipogenic differentiation of bone marrow mesenchymal stem cells (BMSCs). According to a theory of traditional Chinese medicine (TCM), the kidneys contain an “essence” that regulate bone metabolism and generate marrow. Kidney disorders are therefore considered to be a major cause of OP as per the principles of TCM, which recommends kidney-tonifying treatments for OP. The Zuogui pill (ZGP) is a classic kidney-tonifying medication that effectively improves OP symptoms. Studies have shown that ZGP can promote the osteogenic differentiation of BMSCs, providing scientific evidence for the TCM theory linking kidneys with bone metabolism. In this review, we have provided an overview of recent studies that examined the underlying mechanisms of ZGP mediated regulation of BMSC osteogenic and adipogenic differentiation.

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“…However, the long-term use of estrogen increases the risk of breast cancer, endometrial cancer, cardiovascular accident and vascular embolism (19). In recent years, the prevention and treatment of PMOP by traditional Chinese medicine is attracting more and more attention (20,21). It is of great significance to find an estrogen replacement medicine for the prevention and treatment of postmenopausal osteoporosis in traditional medicine.…”

mentioning

confidence: 99%

Mongolian Medicine echinops prevented postmenopausal osteoporosis and induced ER/AKT/ERK pathway in BMSCs

Liu

Wang

Chang

et al. 2018

BST

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Hormone replacement medicine such as traditional Chinese medicine has proven to be effective in decreasing the risk of osteoporosis. Mongolian medicine echinops prevents osteoporosis, but its mechanism remains unclear. In this study, we explored the mechanism underlying echinops prevents and treats postmenopausal osteoporosis. Osteoporosis model was established by ovariectomy in rats. Rats were treated to Echinops (16.26, 32.5, or 65 mg/kg/day) by oral gavage for 3 months. Bone mineral density (BMD) was detected by micro-CT detection of left proximal medial metaphyseal tibia. Hematoxylin and eosin (H&E) and toluidine blue O staining were also performed. Serum levels of E2, ALP and testosterone were examined. Bone marrow-derived bone marrow stem cells (BMSCs) were isolated and treated with echinops-containing serum. Estrogen receptors (ER) including ERα and ERβ in bone specimens and BMSCs were detected by qRT-PCR. Cell viability and colon formation of BMSCs were detected. Expressions of ERα, ERβ, AKT, p-AKT, ERK, and p-ERK in BMSCs were detected by western blot. Results showed that echinops significantly increased trabecular interconnectivity, thickness of trabeculae, and connection of trabecula. Echinops significantly increased BMD and E2, but significantly reduced ALP and testosterone in dose-dependent manners. Echinops induced ERα and ERβ in both bone specimens and BMSCs. Echinops enhanced cell viability and ability of colony formation of BMSCs, and increased ERα, ERβ, p-AKT, and p-ERK. Thus, Mongolian echinops reduced bone loss and delayed the occurrence and development of osteoporosis, and increased ERα, ERβ, p-AKT, and P-ERK in BMSCs. These results provide experimental basis for clinical prevention and treatment of postmenopausal osteoporosis by echniops.

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Effect of Qing’e formula on the in vitro differentiation of bone marrow-derived mesenchymal stem cells from proximal femurs of postmenopausal osteoporotic mice

Cited by 19 publications

References 31 publications

Role of the ER/NO/cGMP Signaling Pathway in the Promotion of Osteogenic Differentiation of Rat Bone Marrow Mesenchymal Stem Cells by Actaea racemosa Extract

Role of the ER/NO/cGMP Signaling Pathway in the Promotion of Osteogenic Differentiation of Rat Bone Marrow Mesenchymal Stem Cells by Actaea racemosa Extract

Mechanisms of Zuogui Pill in Treating Osteoporosis: Perspective from Bone Marrow Mesenchymal Stem Cells

Mongolian Medicine echinops prevented postmenopausal osteoporosis and induced ER/AKT/ERK pathway in BMSCs

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