Effect of pure curcumin, demethoxycurcumin, and bisdemethoxycurcumin on WT1 gene expression in leukemic cell lines

Anuchapreeda, Songyot; Tima, Singkome; Duangrat, Chadarat; Limtrakul, Pornngarm

doi:10.1007/s00280-007-0642-1

Cited by 60 publications

(58 citation statements)

References 39 publications

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“…Hydrogenated l-α-phosphatidylcholine from egg yolk (HEPC), soybean oil, polysorbate 80 (polyoxyethylene (20) sorbitanmonooleate, Tween 80), and chloroform were purchased from NacalaiTesque Inc., Kyoto, Japan. In HEPC, the phospholipid content was more than 99% and the phosphatidylcholine content was approximately 70%.…”

Section: Chemicalsmentioning

confidence: 99%

“…Curcumin is effective for preventing and ameliorating gastric lesions. It also possesses anti-inflammatory [4], antibacterial [5,6], antifungal and antiyeast [7], antihypocholesterolemic [8], anticancer [9][10][11][12], antimutagen [13], antiparasitic [14], antitumorpromoting [15], antiproliferative [16], MDR modulator effects [17], and so on.…”

Section: Introductionmentioning

confidence: 99%

“…The authors found that curcumin is a potent antiproliferative agent for the tested cancer cell lines [19,20]. A recent report indicates that pure curcumin concentration for oncogene target inhibition and inhibitory effect on cancer cell proliferation is ∼15-20 μM [21].…”

Section: Introductionmentioning

confidence: 99%

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Preparation of Lipid Nanoemulsions Incorporating Curcumin for Cancer Therapy

Anuchapreeda

Fukumori

Okonogi

et al. 2012

Journal of Nanotechnology

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104

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The aim of this study was to develop a new formulation of a curcumin lipid nanoemulsion having the smallest particle size, the highest loading, and a good physical stability for cancer chemotherapy. Curcumin lipid nanoemulsions were prepared by a modified thin-film hydration method followed by sonication. Soybean oil, hydrogenated l-α-phosphatidylcholine from egg yolk, and cosurfactants were used to formulate the emulsions. The resultant nanoemulsions showed mean particle diameter of 47-55 nm, could incorporate 23-28 mg curcumin per 30 mL, and were stable in particle size for 60 days at 4• C. The cytotoxicity studies of curucumin solution and curcumin-loaded nanoemulsion using B16F10 and leukemic cell lines showed IC 50 values ranging from 3.5 to 30.1 and 22.2 to 53.7 μM, respectively. These results demonstrated the successful incorporation of curcumin into lipid nanoemulsion particles with small particle size, high loading capacity, good physical stability, and preserved cytotoxicity.

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Section: Chemicalsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Preparation of Lipid Nanoemulsions Incorporating Curcumin for Cancer Therapy

Anuchapreeda

Fukumori

Okonogi

et al. 2012

Journal of Nanotechnology

Self Cite

104

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“…Recently, more and more studies have demonstrated the stronger activity of DMC when compared with curcumin in many aspects (8)(9)(10). Numerous studies have shown that DMC induces cytotoxic effects in many cancer cell lines such as colon (11) and renal cell cancer (12), glioma (9) and leukemic cell lines (13). However, no study exists which shows the effects of DMC on human lung cancer cells, and the role of DMC in inducing cell cycle arrest and apoptosis has never been investigated in detail.…”

Section: Introductionmentioning

confidence: 99%

Demethoxycurcumin induces the apoptosis of human lung cancer NCI-H460 cells through the mitochondrial-dependent pathway

Lien

Liu

et al. 2015

Oncology Reports

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Abstract. Lung cancer is the most common cause of cancerrelated mortality in the US as well as other regions of the world. Curcumin, demethoxycurcumin (DMC) and bisdemethoxycurcumin (BDMC) are the major components of Curcuma longa L. It has been reported that curcumin inhibits the growth of various types of cancer cells in vitro and in vivo. However, the mechanisms involved in the inhibition of cell growth and induced apoptosis by DMC in human lung cancer cells remain unclear. In the present study, we investigated the effect of DMC on cell death via the induction of apoptosis in NCI-H460 human lung cancer cells. Flow cytometric assay was used to examine the total percentage of viable cells, the population of cells in the sub-G1 phase of the cell cycle, the level of reactive oxygen species (ROS), Ca 2+ production, mitochondrial membrane potential (ΔΨm) and caspase activity. Western blotting was used to examine the changes in the expression of cell cycle-and apoptosis-associated proteins. Confocal microscopy was used to examine the translocation of apoptosis-associated proteins. The results indicated that DMC significantly induced cell morphological changes and decreased the percentage of viable NCI-H460 cells and DMC induced apoptosis based on the cell distribution in the sub-G1 phase. Moreover, DMC promoted ROS and Ca 2+ production and decreased the level of ΔΨm and promoted the activities of caspase-3, -8 and -9. The Western blotting results showed that DMC promoted the expression of AIF, Endo G and PARP. The levels of Fas ligand (Fas L) and Fas were also upregulated. Furthermore, DMC promoted expression of ER stress-associated proteins such as GRP78, GADD153, IRE1β, ATF-6α, ATF-6β and caspase-4. Based on the findings, we suggest that DMC may be used as a novel anticancer agent for the treatment of lung cancer in the future.

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“…The mechanism may be that curcumin could suppress the activation of transcription factor NF-B [6][7][8] and signaling molecules related to carcinogenesis [9,10]. In vivo studies on rodent models further confirmed its anti-diabetes [11,12], antiAlzheimer's disease [13][14][15][16][17], and anti-tumorigenesis activities [18][19][20][21][22][23]. In addition, several clinical studies have revealed that curcumin is safe for human even at an oral dosage of 12 g per day [24,25].…”

Section: Introductionmentioning

confidence: 99%

Metabolic and pharmacokinetic studies of curcumin, demethoxycurcumin and bisdemethoxycurcumin in mice tumor after intragastric administration of nanoparticle formulations by liquid chromatography coupled with tandem mass spectrometry

Qiao

et al. 2011

Journal of Chromatography B

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Effect of pure curcumin, demethoxycurcumin, and bisdemethoxycurcumin on WT1 gene expression in leukemic cell lines

Cited by 60 publications

References 39 publications

Preparation of Lipid Nanoemulsions Incorporating Curcumin for Cancer Therapy

Preparation of Lipid Nanoemulsions Incorporating Curcumin for Cancer Therapy

Demethoxycurcumin induces the apoptosis of human lung cancer NCI-H460 cells through the mitochondrial-dependent pathway

Metabolic and pharmacokinetic studies of curcumin, demethoxycurcumin and bisdemethoxycurcumin in mice tumor after intragastric administration of nanoparticle formulations by liquid chromatography coupled with tandem mass spectrometry

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