Effect of Pulsed Wave Low-Level Laser Therapy on Tibial Complete Osteotomy Model of Fracture Healing With an Intramedullary Fixation

Abstract

Background:Fractures pose a major worldwide challenge to public health, causing tremendous disability for the society and families. According to recent studies, many in vivo and in vitro experiments have shown the positive effects of PW LLLT on osseous tissue.Objectives:The aim of this study was to evaluate the outcome of infrared pulsed wave low-level laser therapy (PW LLLT) on the fracture healing process in a complete tibial osteotomy in a rat model, which was stabilized by an intramedullary pin.Materials and Methods:This experimental study was conducted at Shahid Beheshti University of Medical Sciences in Tehran, Iran. We performed complete tibial osteotomies in the right tibias for the population of 15 female rats. The rats were divided randomly into three different groups: I) Control rats with untreated bone defects; II) Rats irradiated by a 0.972 J/cm2 PW LLLT; and III) Rats irradiated by a 1.5 J/cm2 PW LLLT. The right tibias were collected six weeks following the surgery and a three-point bending test was performed to gather results. Immediately after biomechanical examination, the fractured bones were prepared for histological examinations. Slides were examined using stereological method.Results:PW LLLT significantly caused an increase in maximum force (N) of biomechanical repair properties for osteotomized tibias in the first and second laser groups (30.0 ± 15.9 and 32.4 ± 13.8 respectively) compared to the control group (8.6 ± 4.5) LSD test, P = 0.019, P = 0.011 respectively). There was a significant increase in the osteoblast count of the first and second laser groups (0.53 ± 0.06, 0.41 ± 0.06 respectively) compared to control group (0.31 ± 0.04) (LSD test, P = 0001, P = 0.007 respectively).Conclusions:This study confirmed the efficacy of PW LLLT on biomechanical strength, trabecular bone volume, callus volume, and osteoblast number of repairing callus in a complete tibial osteotomy animal model at a relatively late stage of the bone healing process.

Influence of class IV laser therapy on the outcomes of tibial plateau leveling osteotomy in dogs

“…This skewed distribution between treatment groups makes it less likely that the laser treatments improved osteotomy healing. The lack of effect on osteotomy healing supports the findings of another clinical trial in dogs but conflicts with in vivo studies that used rodent models as well as with a clinical trial in man . Many variables can affect bone healing in response to laser therapy, including frequency of application .…”

“…The lack of effect on osteotomy healing supports the findings of another clinical trial in dogs but conflicts with in vivo studies that used rodent models as well as with a clinical trial in man . Many variables can affect bone healing in response to laser therapy, including frequency of application . Chang, Wu et al demonstrated improved fracture healing in human hand and wrist fractures after 5 treatments per week for 2 weeks .…”

A Comprehensive Analysis of Near‐Contact Photobiomodulation Therapy in the Host–Bacteria Interaction Model Using 3D‐Printed Modular LED Platform

“…It has been reported that pulsed wave (PW) light therapy can be more effective than continuous wave (CW) light therapy in some biological systems 26–28. To compare the efficacy of PBM therapy on anti‐inflammation activity in the LPS involved host–bacteria interaction model, we examined the cell viability and cytokine productions under low or high pulse frequency conditions (5 or 500 Hz; Figure 5 ).…”

Comparison of the effects of once‐weekly and once‐daily rhPTH (1–34) injections on promoting fracture healing in rodents

“…All rats underwent surgery to produce mid‐diaphyseal fractures of the left tibiae using a previously reported method . The animals were anesthetized with an intraperitoneal injection of 10% chloral hydrate (3.3 ml/kg).…”

“…All rats underwent surgery to produce mid-diaphyseal fractures of the left tibiae using a previously reported method. 14,15 The animals were anesthetized with an intraperitoneal injection of 10% chloral hydrate (3.3 ml/kg). A 1-cm incision was made anterior to the upper end of the right tibia through the skin and the subcutaneous tissues, and the tibia plateau was exposed under sterile conditions.…”