Effect of pulsatile shear stress on endothelial attachment to native vascular surfaces

Thompson, M. M.; Budd, J. S.; Eady, Sarah L.; James, R. F. L.; Bell, P. R. F.

doi:10.1002/bjs.1800810813

Cited by 26 publications

(15 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The vessel thickness can also be measured in real-time via ultrasonic probe [132]. Duplex ultrasonography techniques have also been adapted to directly measure the flow profile to facilitate FSS calculation [148]. By altering the flow waveforms generated by the perfusion pump, researchers can investigate the effects of steady, pulsatile, or oscillatory FSS of [132,146].…”

Section: Neonatal Rat Pulmonary Fibroblastsmentioning

confidence: 99%

Device-Based In Vitro Techniques for Mechanical Stimulation of Vascular Cells: A Review

Davis

Zambrano

Anumolu

et al. 2015

Journal of Biomechanical Engineering

View full text Add to dashboard Cite

The most common cause of death in the developed world is cardiovascular disease. For decades, this has provided a powerful motivation to study the effects of mechanical forces on vascular cells in a controlled setting, since these cells have been implicated in the development of disease. Early efforts in the 1970 s included the first use of a parallel-plate flow system to apply shear stress to endothelial cells (ECs) and the development of uniaxial substrate stretching techniques (Krueger et al., 1971, "An in Vitro Study of Flow Response by Cells," J. Biomech., 4(1), pp. 31-36 and Meikle et al., 1979, "Rabbit Cranial Sutures in Vitro: A New Experimental Model for Studying the Response of Fibrous Joints to Mechanical Stress," Calcif. Tissue Int., 28(2), pp. 13-144). Since then, a multitude of in vitro devices have been designed and developed for mechanical stimulation of vascular cells and tissues in an effort to better understand their response to in vivo physiologic mechanical conditions. This article reviews the functional attributes of mechanical bioreactors developed in the 21st century, including their major advantages and disadvantages. Each of these systems has been categorized in terms of their primary loading modality: fluid shear stress (FSS), substrate distention, combined distention and fluid shear, or other applied forces. The goal of this article is to provide researchers with a survey of useful methodologies that can be adapted to studies in this area, and to clarify future possibilities for improved research methods.

show abstract

Section: Neonatal Rat Pulmonary Fibroblastsmentioning

confidence: 99%

Device-Based In Vitro Techniques for Mechanical Stimulation of Vascular Cells: A Review

Davis

Zambrano

Anumolu

et al. 2015

Journal of Biomechanical Engineering

View full text Add to dashboard Cite

show abstract

“…More than 70% of seeded ECs are washed away during restoration of the blood flow [Rosenman et al, 1985]. EC detachment from the surface, which essentially occurs during the first 20 min after restoration of the blood flow, is greater at increasing levels of shear stress [Thompson et al, 1994]. Various groups have studied the role of adhesive proteins such as fibronectin, collagen, gelatine, whole ECM and fibrinogen in enhancing the EC attachment.…”

Section: Enhanced Endothelial Attachment and Shear Stress Resistancementioning

confidence: 99%

Fibrin: A Natural Biodegradable Scaffold in Vascular Tissue Engineering

Shaikh¹,

Callanan²,

Kavanagh³

et al. 2008

Cells Tissues Organs

173

110

View full text Add to dashboard Cite

Arterial occlusive disease remains a major health issue in the developed world and a rapidly growing problem in the developing world. Although a growing number of patients are now being effectively treated with minimally invasive techniques, there remains a tremendous pressure on the vascular community to develop a synthetic small-diameter vascular graft with improved long-term patency rates. The field of tissue engineering offers an exciting alternative in the search for living organ replacement structures. Several methodologies have emerged for constructing blood vessel replacements with biological functionality. Common strategies include cell-seeded biodegradable synthetic scaffolds, cell self-assembly, cell-seeded gels and xenogeneic acellular materials. A wide range of materials are being investigated as potential scaffolds for vascular tissue engineering applications. Some are commercialised and others are still in development. Recently, researchers have studied the role of fibrin gel as a three-dimensional scaffold in vascular tissue engineering. This overview describes the properties of fibrin gel in vascular tissue engineering and highlights some recent progress and difficulties encountered in the development of cell fibrin scaffold technology.

show abstract

“…26 Achieving TEVG endothelialization that is stable under physiological loading conditions is challenging, and engineered EC layers frequently delaminate upon exposure to high shear pulsatile flow. 30,37 A range of methods have been employed to enhance stable vascular graft endothelialization, including specialized EC seeding methods 37 and luminal coatings (e.g., fibronectin, collagen). 24 However, only moderate long-term success has been attained with these approaches.…”

Section: Introductionmentioning

confidence: 99%

Approach for Fabricating Tissue Engineered Vascular Grafts with Stable Endothelialization

et al. 2010

View full text Add to dashboard Cite

A major roadblock in the development of tissue engineered vascular grafts (TEVGs) is achieving construct endothelialization that is stable under physiological stresses. The aim of the current study was to validate an approach for generating a mechanically stable layer of endothelial cells (ECs) in the lumen of TEVGs. To accomplish this goal, a unique method was developed to fabricate a thin EC layer using poly(ethylene glycol) diacrylate (PEGDA) as an intercellular "cementing" agent. This EC layer was subsequently bonded to the lumen of a tubular scaffold to generate a bi-layered construct. The viability of bovine aortic endothelial cells (BAECs) through the "cementing" process was assessed. "Cemented" EC layer expression of desired phenotypic markers (AcLDL uptake, VE-cadherin, eNOS, PECAM-1) as well as of injury-associated markers (E-selectin, SM22alpha) was also examined. These studies indicated that the "cementing" process allowed ECs to maintain high viability and expression of mature EC markers while not significantly stimulating primary injury pathways. Finally, the stability of the "cemented" EC layers under abrupt application of high shear pulsatile flow (approximately 11 dyn/cm(2), P (avg) approximately 95 mmHg, DeltaP approximately 20 mmHg) was evaluated and compared to that of conventionally "seeded" EC layers. Whereas the "cemented" ECs remained fully intact following 48 h of pulsatile flow, the "seeded" EC layers delaminated after less than 1 h of flow. Furthermore, the ability to extend this approach to degradable PEGDA "cements" permissive of cell elongation was demonstrated. Combined, these results validate an approach for fabricating bi-layered TEVGs with stable endothelialization.

show abstract

Effect of pulsatile shear stress on endothelial attachment to native vascular surfaces

Cited by 26 publications

References 25 publications

Device-Based In Vitro Techniques for Mechanical Stimulation of Vascular Cells: A Review

Device-Based In Vitro Techniques for Mechanical Stimulation of Vascular Cells: A Review

Fibrin: A Natural Biodegradable Scaffold in Vascular Tissue Engineering

Approach for Fabricating Tissue Engineered Vascular Grafts with Stable Endothelialization

Contact Info

Product

Resources

About