Effect of PUFA on embryo cryoresistance, gene expression and AMPKα phosphorylation in IVF-derived bovine embryos

Darwich, Abdulrahman Al; Perreau, C.; Petit, Marie Hélène; Papillier, Pascal; Dupont, Joëlle; Guillaume, Daniel; Mermillod, Pascal; Guignot, Florence

doi:10.1016/j.prostaglandins.2010.06.002

Cited by 59 publications

(55 citation statements)

References 35 publications

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“…However, similar to the findings of our study, an increased rate of embryonic development has been observed for bovine oocytes, although an optimum range of ALA in that study showed to be 50 μM [3]. In another study in cattle, addition of ALA at 1, 10, and 100 μM concentrations to the culture medium did not improve the cleavage and blastocyst rates [25]. These different findings suggest that ALA might be more significant in the time of maturation.…”

Section: Discussionsupporting

confidence: 86%

“…These different findings suggest that ALA might be more significant in the time of maturation. Furthermore, Al Darwich et al (2010) also found that the viability of the produced bovine embryos was influenced in a positive manner by the presence of ALA in culture medium [25] that may be reflect the anti-apoptotic effect of ALA on mRNA abundance of the BAX and BCL-2.…”

Section: Discussionmentioning

confidence: 90%

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Effect of Linolenic acid during in vitro maturation of ovine oocytes: embryonic developmental potential and mRNA abundances of genes involved in apoptosis

Veshkini

Asadi

Khadem

et al. 2015

J Assist Reprod Genet

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Purpose To study the effect of α-linolenic acid (ALA) on meiotic maturation, mRNA abundance of apoptosis-related (Bax and Bcl-2) molecules, and blastocyst formation in ovine oocytes. Methods A preliminary experiment was conducted to analyze the concentration of ALA in Bsmall^(≤2 mm) and Blarge( ≥6 mm) follicles using gas chromatography/mass spectrometry analysis. The concentration of ALA in small and large follicles was determined to be in a range of 75.4 to 125.7 μM,

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 90%

Effect of Linolenic acid during in vitro maturation of ovine oocytes: embryonic developmental potential and mRNA abundances of genes involved in apoptosis

Veshkini

Asadi

Khadem

et al. 2015

J Assist Reprod Genet

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“…Although these are different cell types, the early embryo cells show high metabolic activity, similarly to tumor cells [18], and it is possible that trans -10, cis- 12 CLA causes some effects in the embryonic cells similar to those reported for somatic cells. The apparent contradiction between potentially beneficial and detrimental effects of trans -10, cis- 12 CLA has not been addressed in previous studies evaluating embryos cultured in the absence of additional antioxidant protection, such as routinely used in the IVEP industry [17,19,20]. …”

Section: Introductionmentioning

confidence: 99%

Trans-10, cis-12 conjugated linoleic acid reduces neutral lipid content and may affect cryotolerance of in vitro- produced crossbred bovine embryos

Batista

Raposo

Campos-Júnior

et al. 2014

J Animal Sci Biotechnol

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BackgroundDue to high neutral lipids accumulation in the cytoplasm, in vitro-produced embryos from Bos primigenius indicus and their crosses are more sensitive to chilling and cryopreservation than those from Bos primigenius taurus. The objective of the present study was to evaluate the effects of trans-10, cis-12 conjugated linoleic acid (CLA) on the development and cryotolerance of crossbred Bos primigenius taurus x Bos primigenius indicus embryos produced in vitro, and cultured in the presence of fetal calf serum. Bovine zygotes (n = 1,692) were randomly assigned to one of the following treatment groups: 1) Control, zygotes cultured in Charles Rosenkrans 2 amino acid (CR2aa) medium (n = 815) or 2) CLA, zygotes cultured in CR2aa medium supplemented with 100 μmol/L of trans-10, cis-12 CLA (n = 877). Embryo development (cleavage and blastocyst rates evaluated at days 3 and 8 of culture, respectively), lipid content at morula stage (day 5) and blastocyst cryotolerance (re-expansion and hatching rates, evaluated 24 and 72 h post-thawing, respectively) were compared between groups. Additionally, selected mRNA transcripts were measured by Real–Time PCR in blastocyst stage.ResultsThe CLA treatment had no effect on cleavage and blastocyst rates, or on mRNA levels for genes related to cellular stress and apoptosis. On the other hand, abundance of mRNA for the 1-acylglycerol-3-phosphate 0-acyltransferase-encoding gene (AGPAT), which is involved in triglycerides synthesis, and consequently neutral lipid content, were reduced by CLA treatment. A significant increase was observed in the re-expansion rate of embryos cultured with trans-10, cis-12 CLA when compared to control (56.3 vs. 34.4%, respectively, P = 0.002). However, this difference was not observed in the hatching rate (16.5 vs. 14.0%, respectively, P = 0.62).ConclusionsThe supplementation with trans-10, cis-12 CLA isomer in culture medium reduced the lipid content of in vitro produced bovine embryos by reducing the gene expression of 1-acylglycerol 3-phosphate 0-acyltransferase (AGPAT) enzyme. However, a possible improvement in embryo cryotolerance in response to CLA, as suggested by increased blastocyst re-expansion rate, was not confirmed by hatching rates.

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“…On the basis of the multi-faceted influences of long-chain PUFAs on membrane properties and membrane-linked processes, considerable additional research is required for optimal concentrations (or more pragmatically, biologically beneficial ranges) to be deduced. Such information could complement and facilitate insights into the roles of endogenous lipids (Sturmey et al, 2009) as well as highlighting their inter-connectedness; a case in point is recent evidence that, in the case of EPA and DHA, these two long-chain PUFAs could have beneficial effects in so far as they regulate the expression of the desaturases (Al Darwich et al, 2010) and potentially therefore the nature of the fatty acid-derived energy supply.…”

Section: Discussionmentioning

confidence: 99%

“…In contrast, the same group had previously found that supplementation with α-linolenic acid enhanced maturation rate and increased bovine blastocyst yield (Marei et al, 2009). In a recent study by Al Darwich et al (2010) enhanced in vitro bovine embryo survival was also obtained with linolenic acid supplementation compared with that achieved in presence of either conjugated linoleic acid (CLA) or docosahexaenoic acid. While conjugated fatty acids are a special case due to their various and intriguing biological influences, they are not a focus for the present study.…”

Section: Introductionmentioning

confidence: 90%

Impact of contrasting fish oil concentrations in the diet on ovine embryo development <i>in vivo</i> and of corresponding diet-specific derivative sera during <i>in vitro</i> culture

McEvoy¹,

Önal²,

Speake³

et al. 2012

J. Anim. Feed Sci.

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In the first of two experiments, numbers of ovulations and day 5 embryo yields were unaffected by fish oil (0, 3 or 6% w/w) in ewe diets; oestradiol concentrations and numbers of large ovarian follicles were increased. Fish oil tended to suppress embryo development and limit blastocyst diameter and cell number but not protein synthesis. In the second experiment IVF-derived embryos were cultured in medium supplemented with foetal calf serum (FCS) or, as in vitro equivalents to Experiment 1, serum from ewes fed rations supplemented with 0 (C), 3 (3F) or 6 (6F)% fish oil. Cleavage rates were not affected by serum source. More Grade 1 to 1.5 blastocysts were produced when using 3F serum (32% of blastocysts) than with C (27%; ns) or 6F (16%; P<0.05) or FCS (10%; P<0.005). It is suggested that at less than 3% inclusion in the diet of ewes, there may be beneficial effects of fish oil fatty acids on embryo production in vivo and of the corresponding ewe serum during in vitro culture. Higher concentrations are counter-productive.

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Effect of PUFA on embryo cryoresistance, gene expression and AMPKα phosphorylation in IVF-derived bovine embryos

Cited by 59 publications

References 35 publications

Effect of Linolenic acid during in vitro maturation of ovine oocytes: embryonic developmental potential and mRNA abundances of genes involved in apoptosis

Effect of Linolenic acid during in vitro maturation of ovine oocytes: embryonic developmental potential and mRNA abundances of genes involved in apoptosis

Trans-10, cis-12 conjugated linoleic acid reduces neutral lipid content and may affect cryotolerance of in vitro- produced crossbred bovine embryos

Impact of contrasting fish oil concentrations in the diet on ovine embryo development <i>in vivo</i> and of corresponding diet-specific derivative sera during <i>in vitro</i> culture

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