Effect of Puerarin on Osteogenic Differentiation in vitro and on New Bone Formation in vivo

Yang, Yanran; Chen, Daiyun; Li, Yilin; Zou, Jinghua; Han, Ruiqi; Li, Hongkun; Zhang, Jun

doi:10.2147/dddt.s379794

Cited by 9 publications

(6 citation statements)

References 66 publications

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“… 17 , 26 Other studies have reported that the cartilage range is increased and chondrocytes are more proliferative under expansive forces. 27 , 28 Both endochondral and intramembranous ossifications participate in bone remodeling processes in the MPS. 29 In cases of endochondral ossification, hypertrophic chondrocytes undergo absorption, invasion of new tissues, and mineralization.…”

Section: Discussionmentioning

confidence: 99%

Suppressing STAT3 activation impairs bone formation during maxillary expansion and relapse

et al. 2023

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Objectives The mid-palatal expansion technique is commonly used to correct maxillary constriction in dental clinics. However, there is a tendency for it to relapse, and the key molecules responsible for modulating bone formation remain elusive. Thus, this study aimed to investigate whether signal transducer and activator of transcription 3 (STAT3) activation contributes to osteoblast-mediated bone formation during palatal expansion and relapse. Methodology In total, 30 male Wistar rats were randomly allocated into Ctrl (control), E (expansion only), and E+Stattic (expansion plus STAT3-inhibitor, Stattic) groups. Micro-computed tomography, micromorphology staining, and immunohistochemistry of the mid-palatal suture were performed on days 7 and 14. In vitro cyclic tensile stress (10% magnitude, 0.5 Hz frequency, and 24 h duration) was applied to rat primary osteoblasts and Stattic was administered for STAT3 inhibition. The role of STAT3 in mechanical loading-induced osteoblasts was confirmed by alkaline phosphatase (ALP), alizarin red staining, and western blots. Results The E group showed greater arch width than the E+Stattic group after expansion. The differences between the two groups remained significant after relapse. We found active bone formation in the E group with increased expression of ALP, COL-I, and Runx2, although the expression of osteogenesis-related factors was downregulated in the E+stattic group. After STAT3 inhibition, expansive force-induced bone resorption was attenuated, as TRAP staining demonstrated. Furthermore, the administration of Stattic in vitro partially suppressed tensile stress-enhanced osteogenic markers in osteoblasts. Conclusions STAT3 inactivation reduced osteoblast-mediated bone formation during palatal expansion and post-expansion relapse, thus it may be a potential therapeutic target to treat force-induced bone formation.

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Section: Discussionmentioning

confidence: 99%

Suppressing STAT3 activation impairs bone formation during maxillary expansion and relapse

et al. 2023

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“…A total of nine male SD rats (specific-pathogen-free) (SPF) (Sibeifu, Beijing, China), weighing 250–300 g, were selected. Preparation of the periodontal defect was as previously described [ 29 , 30 , 31 ]. The alveolar bone defect of about 2 × 1 × 0.8 mm 3 was created in the mesial alveolar bone of the bilateral maxillary first molar mesial root.…”

Section: Methodsmentioning

confidence: 99%

The Experimental Study of Periodontal Ligament Stem Cells Derived Exosomes with Hydrogel Accelerating Bone Regeneration on Alveolar Bone Defect

et al. 2022

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Introduction: this study was conducted to investigate the osteogenic ability of periodontal ligament stem cells (PDLSCs) derived exosomes (PDLSCs-Exos) and the effect of PDLSCs-Exos with hydrogel on alveolar bone defect repairment in the rat. Methods: the PDLSCs were obtained through primary cell culture, and PDLSCs-Exos were purified by the ultracentrifugation method. The CCK-8 kit and ALP staining were used to explore the effect of PDLSCs-Exos on promoting the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs). In vivo, the alveolar bone defect models were made mesial to the bilateral maxillary first molars of rats. MicroCT, HE staining, and Masson staining were used to analyze the new bone at the bone defect of rats. Results: the periodontal ligament stem cells and the periodontal ligament stem cells derived exosomes were successfully extracted. The results of the CCK-8 kit and ALP staining showed PDLSCs-Exos significantly promoted the proliferation osteogenic differentiation of BMSCs. In vivo experiment results revealed that compared with the control group and the hydrogel group, the rats in the hydrogel with exosomes group showed more new bone formation in alveolar bone defects. Conclusion: Periodontal ligament stem cells and exosomes derived from periodontal ligament stem cells were successfully extracted. The results demonstrated that the hydrogel successfully delivered periodontal ligament stem cells derived exosomes for repairing alveolar bone defects in rats in vivo at the initial stage.

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“…11,12 Puerarin (Pue), a candidate drug compound extracted from the plant Pueraria lobata, have strong antioxidant, antiinflammatory and other pharmacological activities. Previous studies have substantiated that Pue can inhibit oxidative stress by inhibiting ATP production and activating AMPK phosphorylation in a streptozotocin-induced mouse model of type I diabetes, 13 regulate NLRP3 inflammatory vesicles through autophagy, which is protective against hyperglycemiainduced chronic vascular disease, 14 mimic an antibacterial peptide, which kills E. coli by binding to lipopolysaccharides and blocking their biological function, 15 and help wound healing by inhibiting NF-κB and MAPK signaling pathways and modulating M2 polarization in macrophages. 16 However, its direct application in wound repair has been limited by low hydrophilicity, poor bioavailability, and low penetration of Pue.…”

Section: Introductionmentioning

confidence: 98%

Design and Self-Assembly of Peptide-Copolymer Conjugates into Nanoparticle Hydrogel for Wound Healing in Diabetes

Lin,

Zhang,

Cai

et al. 2024

IJN

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Background Delayed wound healing in skin injuries has become a significant problem in clinics, seriously affecting and even threatening life and health. Recently, research interest has increased in developing wound dressings containing bioactive compounds capable of improving outcomes for complex healing needs. Methods In this study, Puerarin -loaded nanoparticles (Pue-NPs) were prepared using the cell-penetrating peptide-poly (lactic-co-glycolic acid) (CPP-PLGA) as a drug carrier by the emulsified solvent evaporation method. Then, they were added into poly (acrylic acid) to obtain a self-assembled nanocomposite hydrogels (SANHs) drug delivery system using the co-polymerization method. The particle size, zeta potential, and micromorphology of Pue-NPs were measured; the appearance, mechanical properties, adhesive strength, and biological activity of SANHs were performed. Finally, the potential of SANHs for wound healing was further evaluated in streptozotocin-induced diabetic mice. Results Pue-NPs were regularly spherical, with an average particle size of 134.57 ± 1.42 nm and a zeta potential of 2.14 ± 0.78 mV. SANHs was colorless and transparent with a honeycomb-like porous structure and had an excellent swelling ratio (917%), water vapor transmission rate (3077 g·m −2 ·day −1 ), mechanical properties (Young’s modulus of 18 kPa, elongation at break of 307%), and adhesive strength (15.5 kPa). SANHs exhibited sustained release of Pue over 48h, with a cumulative release of 55.60 ± 6.01%. In vitro tests revealed that the SANHs presented a 92.22% antibacterial rate against Escherichia coli after 4h, and a 61.91% scavenging rate of 1.1-diphenyl-2-trinitrophenylhydrazine (DPPH) radical. In vivo experiments showed that SANHs accelerated wound repair by reducing the inflammatory response at the wound site, promoting angiogenesis, and facilitating epidermal regeneration and collagen deposition. Conclusion In conclusion, we successfully prepared SANHs. Our results show that SANHs have excellent performance and improves wound healing in diabetic mice model, indicating that it can be used to develop an effective strategy for the treatment of diabetic wounds.

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Effect of Puerarin on Osteogenic Differentiation in vitro and on New Bone Formation in vivo

Cited by 9 publications

References 66 publications

Suppressing STAT3 activation impairs bone formation during maxillary expansion and relapse

Suppressing STAT3 activation impairs bone formation during maxillary expansion and relapse

The Experimental Study of Periodontal Ligament Stem Cells Derived Exosomes with Hydrogel Accelerating Bone Regeneration on Alveolar Bone Defect

Design and Self-Assembly of Peptide-Copolymer Conjugates into Nanoparticle Hydrogel for Wound Healing in Diabetes

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