Effect of psychiatric drugs on Daphnia magna oxylipin profiles

Garreta-Lara, Elba; Checa, Antonio; Fuchs, David; Tauler, Romà; Lacorte, Sı́lvia; Wheelock, Craig E.; Barata, Carlos

doi:10.1016/j.scitotenv.2018.06.333

Cited by 18 publications

(15 citation statements)

References 54 publications

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“…Hence, the biological role of these metabolites and their underlying biochemical pathways in this species remains to be investigated. However, in another invertebrate, Daphnia magna, several oxylipins, which include 9-HODE, 13-HODE, 9-HOTrE and 13-HOTrE, were shown to increase following a single exposure to different psychiatric drugs (Garreta-Lara et al 2018), whereas in the present study, decreased levels were observed for the same compounds. We speculate that effluent exposure led to LOX inhibition by pharmaceuticals targeting LOX enzymes, which, in turn, resulted in decreased levels of the five oxylipins.…”

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confidence: 79%

“…Inhibitor experiments, similar to the studies performed on other species (Knight et al 1999), may provide valuable insights into oxylipin biosynthesis in damselfly larvae. To verify enzymatic activity, chiral LC-MS/MS analysis of monohydroxy metabolites, such as 9-HODE and 13-HODE, can also be used since it enables identification of (S) (enzymatic) and (R) (non-enzymatic) enantiomers (Garreta-Lara et al 2018). In the present study, control group exposure was carried out in tap water instead of lake water to minimise the uncertainty arising from the potential presence of pollutants in the lake water.…”

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confidence: 99%

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Novel metabolomic method to assess the effect-based removal efficiency of advanced wastewater treatment techniques

et al. 2020

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Environmental contextAdvanced wastewater treatment is required to remove pharmaceuticals and many other consumer chemicals from wastewater effluent. There are conflicting findings, however, on the toxicity of treated effluent, and its effect on living organisms is often neglected. We show that the effect-based removal efficiency of wastewater treatment technologies can be assessed by metabolomic methods, and that this approach contributes to a safer and more controlled water quality. AbstractThere are conflicting findings on the toxicity of effluent from wastewater treatment plants, and only limited possibilities for assessing the effect-based removal efficiency (EBRE) of different treatment techniques. We describe a metabolomics approach to detect perturbations in fatty acid catabolic pathways as a proxy for biological effects. Metabolites in three fatty acid pathways were analysed in a common damselfly larva (Coenagrion hastulatum) by liquid chromatography coupled to mass spectrometry. The larvae were exposed for one week to either conventionally treated effluent (activated sludge treatment), effluent additionally treated with ozone, or effluent additionally treated with biochar filtration, and results were compared with those from tap water control exposure. Five lipoxygenase-derived oxylipins (9,10,13-TriHOME, 9,12,13-TriHOME, 9-HODE, 9-HOTrE, and 13-HOTrE) decreased in response to conventionally treated effluent exposure. By using an additional treatment step, oxylipin levels were restored with exception of 9,10,13-TriHOME (ozonated effluent), and 9-HOTrE and 13-HOTrE (effluent filtered with biochar). Thus, exposure to wastewater effluent affected fatty acid metabolite levels in damselfly larvae, and a subset of the analysed metabolites may serve as indicators for biological effects in biota in response to effluent exposure. To that effect, our findings suggest a new metabolomics protocol for assessing EBRE.

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confidence: 99%

Novel metabolomic method to assess the effect-based removal efficiency of advanced wastewater treatment techniques

et al. 2020

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“…As both ARA and EPA serve as precursors for eicosanoids (Heckmann et al , b ; Schlotz et al ; Garreta‐Lara et al ), they play an important role for Daphnia ’s reproduction and the immune system (Martin‐Creuzburg et al ; Schlotz et al ; Fink and Windisch ). For example, ARA was shown to improve the survival and reproduction of D. magna exposed to an opportunistic bacterial pathogen (Schlotz et al ).…”

Section: Discussionmentioning

confidence: 99%

Equal relevance of omega‐3 and omega‐6 polyunsaturated fatty acids for the fitness of Daphnia spp.

Ilić

Werner

Fink

2019

Limnology & Oceanography

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Essential polyunsaturated fatty acids (PUFAs) have been recognized as a crucial factor that determines the trophic transfer efficiency in plankton communities. As many animals cannot synthesize the classes of ω3‐ and ω6‐PUFAs, the dietary availability of these PUFAs can constrain the fitness of freshwater zooplankton such as Daphnia spp. In particular, eicosapentaenoic acid (EPA, 20:5ω3) is considered to be a crucial determinant of the transfer of biomass at the freshwater plant‐herbivore interface. In contrast to ω3‐PUFAs, the group of ω6‐PUFAs has previously been considered to be of less ecological relevance, although the potential role of the ω6‐PUFA arachidonic acid (ARA, 20:4ω6) remains controversial. To investigate its potential role, we conducted dose‐response growth experiments with two Daphnia species, D. pulex and D. magna, supplemented with EPA or ARA, which allowed us to calculate EPA and ARA saturation thresholds for growth and reproduction of both Daphnia species. Our results provide evidence that not only the availability of ω3‐PUFAs, but also the availability of a ω6‐PUFA, namely ARA, can limit both the growth and reproduction of Daphnia spp. to an equal extent. The saturation thresholds for growth and reproduction were consistently, but not significantly, higher for EPA than for ARA in both Daphnia species. As shifts in phytoplankton community composition might result in environmental fluctuations in the dietary availability of ω3‐ and ω6‐PUFAs, our findings present a significant step in understanding the consequences of the ongoing global biodiversity loss for trophic transfer efficiency at the phytoplankton‐zooplankton interface.

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“…They regulate numerous metabolic functions, such as reproduction, inflammation, and immune cell behavior, in both vertebrates and invertebrates (Heckmann et al 2008 ; Dennis and Norris 2015 ). Oxylipins have been studied in other invertebrate species such as the crustacean Daphnia magna (Heckmann et al 2008 ; Garreta-Lara et al 2018 ) and the barnacle Balanus amphitrite (Knight et al 1999 ). In fish, levels of a group of oxylipins (prostaglandins) were altered after exposure to wastewater treatment plant effluent (David et al 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Oxylipins at intermediate larval stages of damselfly Coenagrion hastulatum as biochemical biomarkers for anthropogenic pollution

Späth

Brodin

Červený

et al. 2021

Environ Sci Pollut Res

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Aquatic pollution resulting from anthropogenic activities requires adequate environmental monitoring strategies in sentinel organisms. Thus, biochemical biomarkers have been used as early-warning tools of biological effects in aquatic organisms. However, before using these markers for environmental monitoring, knowledge about their developmental variation is vital. In this study, we assessed baseline levels and developmental variations of a group of potential biomarkers, oxylipins, during the lifespan of the Northern damselfly (Coenagrion hastulatum) using liquid chromatography-tandem mass spectrometry. Effects of wastewater exposure on baseline levels were studied in a subset of damselflies to investigate the responsiveness due to anthropogenic pollution. Thirty-eight oxylipins deriving from four polyunsaturated fatty acids via two enzymatic pathways were detected in damselflies at three larval stages and in the adult form. Overall, oxylipin baseline levels showed developmental variation, which was lowest in the intermediate larval stages. Effects of exposure to wastewater effluent on oxylipin baseline levels were dependent on the life stage and were greatest in the early and intermediate larval stages. The study provides first insights into oxylipin profiles of damselflies at different stages of development and their developmental variation. Based on our results, we propose further strategies for incorporating oxylipins in damselfly larvae as biochemical markers for anthropogenic pollution. Graphical abstract

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Effect of psychiatric drugs on Daphnia magna oxylipin profiles

Cited by 18 publications

References 54 publications

Novel metabolomic method to assess the effect-based removal efficiency of advanced wastewater treatment techniques

Novel metabolomic method to assess the effect-based removal efficiency of advanced wastewater treatment techniques

Equal relevance of omega‐3 and omega‐6 polyunsaturated fatty acids for the fitness of Daphnia spp.

Oxylipins at intermediate larval stages of damselfly Coenagrion hastulatum as biochemical biomarkers for anthropogenic pollution

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