Effect of PRP and PPP on proliferation and migration of human chondrocytes and synoviocytes in vitro

Abstract

Cartilage tissue engineering can provide substantial relief to people suffering from degenerative cartilage disease, such as osteoarthritis. The autologous platelet-rich plasma (PRP) application appears to improve cartilage healing due to its ability to positively influence cellular mechanisms, mainly in cells from synovium and cartilage. Primary cultures of human synovial fluid stem cells (synoviocytes, SCs) and chondrocytes (CCs) were exposed to various concentrations of non-activated PRP and plateletpoor plasma (PPP) prepared by apheresis. Cell proliferation and migration were evaluated in real-time with the non-invasive xCELLigence System. It was found that PRP had a similar effect on the growth of cells as fetal bovine serum (FBS). Surprisingly, our proliferation assay results indicated that 50% PPP had the largest effect on both cell types, with a statistically significant increase in cell number (P<0.001) compared to the (0% FBS) in vitro control. The migratory ability of SCs was significantly enhanced with 10% PRP and 0.8% hyaluronic acid (HA). HA also augmented migration of CCs. In summary, these results demonstrate that directed cell proliferation and migration are inducible in human articular CCs and SCs, and that both platelet-derived fractions may exert a positive effect and modulate several cell responses that are potentially involved in tissue integration during cartilage repair.

Platelet-Rich Fibrin Facilitates Rabbit Meniscal Repair by Promoting Meniscocytes Proliferation, Migration, and Extracellular Matrix Synthesis

“…In the scratch wound healing assay, PRFM at different concentrations (50% and 100%) could induce significant meniscocyte migration in culture similar to FBSM. In the transwell migration assay, normal PRF could successfully induce significant meniscocytes migration in culture, as that of PRP reported previously [ 38 ]. The growth factors and chemokines originating from PRF enhance the migration of meniscocytes [ 38 ].…”

“…In the transwell migration assay, normal PRF could successfully induce significant meniscocytes migration in culture, as that of PRP reported previously [ 38 ]. The growth factors and chemokines originating from PRF enhance the migration of meniscocytes [ 38 ]. Our results are comparable to other studies that showed the effects of fetal bovine serum on cell migration and the proliferation of porcine meniscal cells in microwound and repair models.…”

Basics of isolation and cultivation of chondrocytes according to good laboratory practice

“…Collagenase type II used in our study did not impair the detection of the CD90, CD44 and CD45 markers. We have confirmed expression of the hyaluronan receptor CD44 and CD 90 (Thy-1) on chondrocytes (1P) as was previously reported (14,15,16). The hyaluronan receptor CD44 belongs to the polymorphic family of CD44 glycoproteins, which have been implicated in a variety of cellular functions including adhesion to hyaluronan and collagen, and which is present in normal chondrocytes (15).…”

Single-Stage Cartilage Repair Using Platelet-Rich Fibrin Scaffolds With Autologous Cartilaginous Grafts

“…8 The growth factors and chemokines originating from PRF enhance the migration of chondrocytes. 2 An ex vivo explant culture model mimicking in vivo microenvironment also confirmed the ability of cellular migration in the tissue level. Cell outgrowth was noted from the minced tissue fragments, and adherence of chondrocytes onto the porous PRF scaffold was observed thereafter.…”