Effect of protocatechuic acid on insulin responsiveness and inflammation in visceral adipose tissue from obese individuals: possible role for PTP1B

Ormazábal, Paulina; Scazzocchio, Beatrice; Varı̀, Rosaria; D’Archivio, Massimo; Silecchia, Gianfranço; Iacovelli, Annunziata; Giovannini, Claudio; Masella, Roberta

doi:10.1038/s41366-018-0075-4

Cited by 55 publications

(39 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…PCA exerted positive regulation of PI3K/AKT and glucose uptake through GLUT4 translocation . PTP1B activity reduction was also shown in AT after in vivo treatment with PCA …”

Section: Discussionmentioning

confidence: 80%

See 1 more Smart Citation

Cocoa Shell Aqueous Phenolic Extract Preserves Mitochondrial Function and Insulin Sensitivity by Attenuating Inflammation between Macrophages and Adipocytes In Vitro

Rebollo‐Hernanz

Zhang

Aguilera

et al. 2019

Molecular Nutrition Food Res

View full text Add to dashboard Cite

Scope: The aim is to assess the action of an aqueous extract from cocoa shell (CAE) and its main phenolic compounds to prevent the loss of obesity-induced mitochondrial function and insulin sensitivity, targeting inflammation between macrophages-adipocytes in vitro. Methods and results: CAE (31-500 µg mL −1 ) inhibits 3T3-L1 adipocytes lipid accumulation and induces browning during differentiation. LPS-stimulated RAW264.7 macrophages show reduced inducible nitric oxide synthase and cyclooxygenase-2 expression and lowered pro-inflammatory cytokine production when treated with CAE and pure phenolics. Inflammatory crosstalk created by stimulating adipocytes with macrophage-conditioned media (CM) is arrested; CAE diminishes tumor necrosis factor-α (67%) and promotes adiponectin secretion (12.3-fold). Mitochondrial function, measured by reactive oxygen species production, mitochondrial content, and activity, is preserved in CM-treated adipocytes through up-regulating peroxisome proliferator-activated receptor gamma coactivator 1-α expression. Increases in insulin receptor (9-fold), phosphoinositide 3-kinase (3-fold), protein kinase B (4-fold) phosphorylation, and a decrease in insulin receptor substrate 1 serine phosphorylation induce increased glucose uptake (34%) and glucose transporter 4 translocation (14-fold) in CM-induced adipocytes. Conclusion: CAE phenolics promote a beige phenotype in adipocytes. Macrophages-adipocytes inflammatory interaction is reduced preventing mitochondrial dysfunction and insulin resistance. For the first time, CAE shows a positive effect on adipogenesis and inflammation-related disorders.

show abstract

“…PCA exerted positive regulation of PI3K/AKT and glucose uptake through GLUT4 translocation . PTP1B activity reduction was also shown in AT after in vivo treatment with PCA …”

Section: Discussionmentioning

confidence: 80%

“…Long‐term cocoa supplementation reduced adipose tissue inflammation in high‐fat‐fed mice by down‐regulating NF‐κB gene expression and modulating eicosanoid metabolism . PCA has been associated with an increase in adiponectin secretion in obese subjects . Likewise, theobromine could be exerting anti‐inflammatory effects.…”

Section: Discussionmentioning

confidence: 99%

Cocoa Shell Aqueous Phenolic Extract Preserves Mitochondrial Function and Insulin Sensitivity by Attenuating Inflammation between Macrophages and Adipocytes In Vitro

Rebollo‐Hernanz

Zhang

Aguilera

et al. 2019

Molecular Nutrition Food Res

View full text Add to dashboard Cite

show abstract

“…Treatment with Gallic acid on db/db mice and fructoseadministered rats facilitated glucose homeostasis, improved insulin sensitivity, reduced obesity, reduced elevated blood pressure and decreased cholesterol levels, as well as inducing adipogenesis in 3T3-L1 adipocytes (Variya et al, 2019). A strong body of evidence supports the biological activity in vitro of Protocatechuic acid as an antioxidant and insulin-mimetic molecule in different biological models (Ormazabal et al, 2018).…”

Section: Identification and Quantification Of Phenolic And Heterocyclmentioning

confidence: 93%

Hypoglycaemic activity of preheated (roasting) Aframomum citratum (C. Pereira) K. Schum and Tetrapleura tetraptera (Schumach Thonn.) fruits beverage on Streptozotocin-induced rats

Eyenga¹,

Takuissu²,

Ziyyat³

et al. 2020

J. Pharmacognosy Phytother.

View full text Add to dashboard Cite

Tetrapleura tetraptera and Aframomum citratum fruits are used as spices and in the traditional pharmacopeia in Cameroon. They are mostly combined and preheated for many purposes while cooking. The effects of roasting on the phenolic content of spices, phenolic profile of a beverage made with the mix of roasted spices and its hypoglycaemic activity on diabetes complications were investigated. Aqueous extract (infusion: 100°C; 1/5 g/ml) was prepared, and the total polyphenols and flavonoids contents before and after heat treatment were quantified. Four formulations of T. tetraptera/A. citratum (95/5; 90/10; 85/15; 80/20) were assessed and the in vitro glucose adsorption capacity of the extracts was studied to retain the most effective formulation (AEF). Phenolic and derivatives compounds of AEF were analysed using HPLC-DAD methodology. The AEF hypoglycaemic activity (250 mg/kg bw) and the various complications of hyperglycaemia after Streptozotocin induction (55 mg/kg) were examined on rats. Significant (p<0.05) improvements in phytochemical content were noticed for T. tetraptera and AEF extracts after heat treatment. Among the phenolic compounds identified, flavone, protocatechic, chlorogenic, p-Coumaric acids, and naringin showed the highest concentrations. Significantly, the effect of AEF is the same as the administration of AEF-Glibenclamide (Glibenclamide) and Acarbose after 4 h when assessing the anti-hyperglycaemic test. The influence of Glibenclamide (the reference drug) is significantly improved by AEF, and this synergy has the best antihyperglycaemic, hypoglycaemic, hypotriglyceridaemic potential and boosts catalase, superoxide dismutase activities while reducing Malondialdehyde (liver, pancreas, haemolysates) and hydroperoxide (liver, plasma, and kidney) contents compared with the use of single AEF.

show abstract

“…Indeed, such metabolites derived from anthocyanins have also been shown to have biological properties against diabetes. The effect of protocatechuic acid (PCA) in the chronic inflammation in visceral adipose tissue (VAT) that may lead to insulin resistance and T2DM was evaluated [ 183 ]. The results showed that this metabolite restored the lack of insulin response in the obese-VAT individuals, by increasing the phosphorylation of key factors for insulin sensitivity, such as IRS-1 and Akt, which may be due to the inhibition of protein tyrosine phosphatase 1B activity, a well-known insulin signaling regulator.…”

Section: Why Anthocyanins?mentioning

confidence: 99%

Anthocyanins as Antidiabetic Agents—In Vitro and In Silico Approaches of Preventive and Therapeutic Effects

et al. 2020

View full text Add to dashboard Cite

Many efforts have been made in the past two decades into the search for novel natural and less-toxic anti-diabetic agents. Some clinical trials have assigned this ability to anthocyanins, although different factors like the food source, the amount ingested, the matrix effect and the time of consumption (before or after a meal) seem to result in contradictory conclusions. The possible mechanisms involved in these preventive or therapeutic effects will be discussed—giving emphasis to the latest in vitro and in silico approaches. Therapeutic strategies to counteract metabolic alterations related to hyperglycemia and Type 2 Diabetes Mellitus (T2DM) may include: (a) Inhibition of carbohydrate-metabolizing enzymes; (b) reduction of glucose transporters expression or activity; (c) inhibition of glycogenolysis and (d) modulation of gut microbiota by anthocyanin breakdown products. These strategies may be achieved through administration of individual anthocyanins or by functional foods containing complexes of anthocyanin:carbohydrate:protein.

show abstract

Effect of protocatechuic acid on insulin responsiveness and inflammation in visceral adipose tissue from obese individuals: possible role for PTP1B

Cited by 55 publications

References 54 publications

Cocoa Shell Aqueous Phenolic Extract Preserves Mitochondrial Function and Insulin Sensitivity by Attenuating Inflammation between Macrophages and Adipocytes In Vitro

Cocoa Shell Aqueous Phenolic Extract Preserves Mitochondrial Function and Insulin Sensitivity by Attenuating Inflammation between Macrophages and Adipocytes In Vitro

Hypoglycaemic activity of preheated (roasting) Aframomum citratum (C. Pereira) K. Schum and Tetrapleura tetraptera (Schumach Thonn.) fruits beverage on Streptozotocin-induced rats

Anthocyanins as Antidiabetic Agents—In Vitro and In Silico Approaches of Preventive and Therapeutic Effects

Contact Info

Product

Resources

About