Effect of Protein Adsorption on the Fluorescence of Ultrasmall Gold Nanoclusters

Shang, L.; Brandholt, S.; Stockmar, F.; Trouillet, Vanessa; Bruns, Michael; Nienhaus, G. Ulrich

doi:10.1002/smll.201400652

Cited by 30 publications

(39 citation statements)

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“…The total increase of R H by ~3 nm revealed a HSA monolayer around the NPs, in which the proteins covered the NP surface with their large triangular faces. FCS measurements of HSA interacting with NPs with similar size but different cores (Au and Fe 3 O 4 ) and surface functionalities (carboxylic acid, amine, PEG polymer, zwitterionic ligand, and lipid) always showed a HSA monolayer enshrouding the NPs . We also studied other prevalent plasma proteins of a different size and charge, such as apotransferrin (apo‐TF), apolipoprotein A‐I, apolipoprotein E3, apolipoprotein E4, and complement component 3 .…”

Section: In Situ Quantification Of the Corona Thicknessmentioning

confidence: 99%

The protein corona on nanoparticles as viewed from a nanoparticle‐sizing perspective

Wang

Lin

Nienhaus

et al. 2017

WIREs Nanomed Nanobiotechnol

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Most surfaces of engineered nanoparticles (NPs) are reactive toward biomolecules. Therefore, whenever NPs become immersed in biological fluids, proteins and other biomolecules bind to the NP surface, forming an adsorption layer (biomolecular corona) that modifies the NPs' physicochemical properties and subsequent interactions with living systems. Its exploration is a formidable endeavor owing to the enormous diversity of engineered NPs in terms of their physicochemical properties and the vast number of biomolecules available in biofluids that may bind to NPs with widely different strengths. Significant progress has been made in our understanding of the biomolecular corona, but even very basic issues are still controversially debated. In fact, there are divergent views of its microscopic structure and dynamics, even on physical properties, such as its thickness. As an example, there is no agreement on whether proteins form monolayers or multilayers upon adsorption. In our quantitative studies of NP-protein interactions by in situ fluorescence correlation spectroscopy (FCS) with highly defined model NPs and important serum proteins, we have universally observed protein monolayer formation around NPs under saturation or even oversaturation conditions. Here, we critically discuss biomolecular corona characterization using FCS and dynamic light scattering and identify challenges and future opportunities. Further careful, quantitative experiments are needed to elucidate the mechanisms of biomolecular corona formation and to characterize its structure. This article is categorized under: Nanotechnology Approaches to Biology > Nanoscale Systems in Biology.

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Section: In Situ Quantification Of the Corona Thicknessmentioning

confidence: 99%

The protein corona on nanoparticles as viewed from a nanoparticle‐sizing perspective

Wang

Lin

Nienhaus

et al. 2017

WIREs Nanomed Nanobiotechnol

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“…Similarly, emission from fluorescent nanoparticles can also be used to obtain the binding constant. Shang et al discovered that the presence of human serum albumin (HSA) could enhance the photoluminescence from ultrasmall gold nanoclusters (AuNC), the emission enhancement was analyzed using the Hill's equation and the equilibrium dissociation was obtained (Figure (a)) . Similar technique was also used to compare the binding affinity of AuNC to HSA with surface modifications (different surface charges), and the results showed that cationic HSA had the highest affinity while anionic HSA had the lowest .…”

Section: Surface Interactions Of Nanomaterials—analytical and Physicomentioning

confidence: 99%

Biological and environmental surface interactions of nanomaterials: characterization, modeling, and prediction

Chen

Riviere

2016

WIREs Nanomed Nanobiotechnol

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The understanding of nano-bio interactions is deemed essential in the design, application, and safe handling of nanomaterials. Proper characterization of the intrinsic physicochemical properties, including their size, surface charge, shape, and functionalization, is needed to consider the fate or impact of nanomaterials in biological and environmental systems. The characterizations of their interactions with surrounding chemical species are often hindered by the complexity of biological or environmental systems, and the drastically different surface physicochemical properties among a large population of nanomaterials. The complexity of these interactions is also due to the diverse ligands of different chemical properties present in most biomacromolecules, and multiple conformations they can assume at different conditions to minimize their conformational free energy. Often these interactions are collectively determined by multiple physical or chemical forces, including electrostatic forces, hydrogen bonding, and hydrophobic forces, and calls for multidimensional characterization strategies, both experimentally and computationally. Through these characterizations, the understanding of the roles surface physicochemical properties of nanomaterials and their surface interactions with biomacromolecules can play in their applications in biomedical and environmental fields can be obtained. To quantitatively decipher these physicochemical surface interactions, computational methods, including physical, statistical, and pharmacokinetic models, can be used for either analyses of large amounts of experimental characterization data, or theoretical prediction of the interactions, and consequent biological behavior in the body after administration. These computational methods include molecular dynamics simulation, structure-activity relationship models such as biological surface adsorption index, and physiologically-based pharmacokinetic models. WIREs Nanomed Nanobiotechnol 2017, 9:e1440. doi: 10.1002/wnan.1440 For further resources related to this article, please visit the WIREs website.

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“…Furthermore, whereas bulk gold is diamagnetic, some AuQDs exhibit magnetic properties (4,6). However, the clear therapeutic and imaging potential of AuQDs in vivo has been undermined by their unfavorable biointeractions and lack of stability in aqueous solvents (5,7). In biological environments AuQDs tend to aggregate rapidly, reverting to larger gold nanoparticles (AuNPs) (8) and/or bind to protein, which negatively affects their cytotoxicity (7).…”

mentioning

confidence: 99%

“…However, the clear therapeutic and imaging potential of AuQDs in vivo has been undermined by their unfavorable biointeractions and lack of stability in aqueous solvents (5,7). In biological environments AuQDs tend to aggregate rapidly, reverting to larger gold nanoparticles (AuNPs) (8) and/or bind to protein, which negatively affects their cytotoxicity (7). To retain their advantages, AuQDs require a protective, stabilizing framework that allows proficient biological interactions.…”

mentioning

confidence: 99%

Gold–silica quantum rattles for multimodal imaging and therapy

Hembury

Chiappini

Bertazzo

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

110

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Gold quantum dots exhibit distinctive optical and magnetic behaviors compared with larger gold nanoparticles. However, their unfavorable interaction with living systems and lack of stability in aqueous solvents has so far prevented their adoption in biology and medicine. Here, a simple synthetic pathway integrates gold quantum dots within a mesoporous silica shell, alongside larger gold nanoparticles within the shell's central cavity. This "quantum rattle" structure is stable in aqueous solutions, does not elicit cell toxicity, preserves the attractive near-infrared photonics and paramagnetism of gold quantum dots, and enhances the drug-carrier performance of the silica shell. In vivo, the quantum rattles reduced tumor burden in a single course of photothermal therapy while coupling three complementary imaging modalities: near-infrared fluorescence, photoacoustic, and magnetic resonance imaging. The incorporation of gold within the quantum rattles significantly enhanced the drug-carrier performance of the silica shell. This innovative material design based on the mutually beneficial interaction of gold and silica introduces the use of gold quantum dots for imaging and therapeutic applications.nanomedicine | hybrid nanoparticle | cancer nanotechnology | gold quantum dots | mesoporous silica A lthough gold's potential in nanotechnology has been recognized for many decades (1, 2), new insights into the unique properties of gold nanoparticles (NPs) of less than 2 nm have just recently started to emerge (3, 4). Such extremely small gold NPs could be transformative for a broad set of applications ranging from energy production and storage to catalysis and health care (3). As the size of gold NPs decreases below 2 nm, the quantization of their conduction band leads to molecule-like properties (3). These quantum-sized gold NPs (or gold quantum dots, AuQDs) absorb light in the near-infrared (NIR) biological window (650-900 nm) (2) and convert it into photons and heat (5). Furthermore, whereas bulk gold is diamagnetic, some AuQDs exhibit magnetic properties (4, 6). However, the clear therapeutic and imaging potential of AuQDs in vivo has been undermined by their unfavorable biointeractions and lack of stability in aqueous solvents (5, 7). In biological environments AuQDs tend to aggregate rapidly, reverting to larger gold nanoparticles (AuNPs) (8) and/or bind to protein, which negatively affects their cytotoxicity (7). To retain their advantages, AuQDs require a protective, stabilizing framework that allows proficient biological interactions.Recently, new emphasis has been placed on hybrid NP systems, where multiple nanomaterials are assembled to create multimodal systems that exhibit the combined qualities of the component modules (9-11). These constructs promise to integrate various functionalities by incorporating different nanomaterials into a single, efficient, multimodal system (12, 13). However, these systems usually accumulate the specific functionalities of their component modules through multiple steps in their ...

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Effect of Protein Adsorption on the Fluorescence of Ultrasmall Gold Nanoclusters

Cited by 30 publications

References 0 publications

The protein corona on nanoparticles as viewed from a nanoparticle‐sizing perspective

The protein corona on nanoparticles as viewed from a nanoparticle‐sizing perspective

Biological and environmental surface interactions of nanomaterials: characterization, modeling, and prediction

Gold–silica quantum rattles for multimodal imaging and therapy

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