Effect of prostaglandins on uterine contractions in the estrous ewe

Rexroad, Caird E.; Barb, C. R.

doi:10.1016/0093-691x(75)90070-9

Cited by 9 publications

(1 citation statement)

References 13 publications

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“…Treatment effects on myometrial contractions were evaluated by three criteria: amplitude (mg), frequency (number/min) and duration (s) (Rexroad & Barb, 1975;Kawarabayashi et al, 1989). Data on con¬ tractions were analysed by analysis of variance procedures for a split-split-slot design using the General Linear Models procedure of the Statistical Analysis System (Snedecor & Cochran, 1980).…”

Section: Introductionmentioning

confidence: 99%

Effects of human chorionic gonadotrophin on contractile activity of steroid-primed pig myometrium in vitro

Flowers

Ziȩcik²,

Caruolo³

1991

Reproduction

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Summary. We examined the effects of (a) oestrogen and progesterone on concentrations of luteinizing hormone/human chorionic gonadotrophin (LH/hCG) receptors in uterine smooth muscle in vivo and (b) hCG on spontaneous myometrial contractions in vitro. Ovariectomized gilts received 2 ml corn oil (control; n = 5), 2 mg oestradiol benzoate (n = 6) or 20 mg progesterone (n = 5) for 5 days. Gilts were hysterectomized 8 h after the last injection and longitudinal sections of myometrium were incubated in modified Krebs' solution with 0 or 10 i.u. of hCG (n = 10/gilt) for 4 h at 37\s=deg\Cin 95% O2:5% CO2. After incubation, myometrial sections were placed in a tissue chamber perfused with Krebs' solution and mechanical activity was recorded for 30 min. Cell membrane fractions were prepared from myometrial tissue not used for in-vitro studies and analysed for LH/hCG receptors. Treatment with oestradiol benzoate increased (P < 0\m=.\01) the number of LH/hCG-binding sites compared with gilts receiving corn oil or progesterone. Incubation of myometrium with hCG reduced (P < 0\m=.\01) the frequency and amplitude of spontaneous uterine contractions in gilts treated with oestradiol benzoate. In contrast, hCG had no effect (P > 0\m=.\05) on the pattern of myometrial contractions in gilts given corn oil or progesterone. These results indicate that oestradiol promotes the synthesis of LH/hCG receptors in pig myometrium and incubation of oestrogen-primed tissue with hCG has a quiescent effect on myometrial contractility.

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Section: Introductionmentioning

confidence: 99%