Effect of prostaglandins on the production of collagenase by rabbit uterine cervical fibroblasts

Goshowaki, Hideko; Ito, Akira; Mori, Yoshihide

doi:10.1016/0090-6980(88)90106-2

Cited by 33 publications

(13 citation statements)

References 16 publications

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“…These results were confirmed by Kleissl et al when they observed decrease of hydroxyprolin concentration during labour [14]. The presence of collagenases and other proteases in the human cervix and in that of other mammals has been demonstrated in numerous biochemical in vestigations [7,9,15,18,23,24,27,28,34,37,38]. Although a cervical collagenase has been isolated [13] it had so far not been possible to directly demonstrate elevated collagenase activity in the cervix during labour.…”

Section: Discussionsupporting

confidence: 85%

Collagenase activity in the cervix of non-pregnant and pregnant women

Osmers

Rath

Adelmann-Grill

et al. 1990

Arch Gynecol Obstet

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Cervical biopsies were obtained from non pregnant patients and from pregnant at various stages of gestation and during labour. The tissues were extract with a Ca(++)-containing buffer, and collagenase activity was determined in these extracts using a solid phase assay in which triple helical 125I-labelled collagen was cleaved. Collagenase was detected in all samples but significantly elevated activity was only present in labour at 6-8 cm cervical dilatation. This provides direct evidence for the crucial role of specific collagen degradation during cervical ripening and dilatation.

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Section: Discussionsupporting

confidence: 85%

Collagenase activity in the cervix of non-pregnant and pregnant women

Osmers

Rath

Adelmann-Grill

et al. 1990

Arch Gynecol Obstet

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“…PGF 2 a increases glycosaminoglycans and the activity of hyaluronic synthases [36]. PGE 2 has been reported to stimulate "collagenase activity" [20]. However, PGE 2 may not actually stimulate collagenase activity directly [39,40].…”

Section: Discussionmentioning

confidence: 99%

Changes in cervical resistance and collagen fluorescence during gestation in rats

Shi

Saade³

et al. 1999

Journal of Perinatal Medicine

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During pregnancy the role of the cervix shifts between two opposing functions. Throughout most of gestation, the cervix is rigid and resists tension in order to maintain the products of conception inside the uterus. At term, however, cervical function changes drastically in order to accommodate stretch and delivery. The events that control cervical function are not known. The aim of this study was to characterize changes in cervical resistance and collagen content during pregnancy in the rat. To determine the change in cervical resistance, non-pregnant and timed pregnant Sprague Dawley rats were sacrificed at various times. Their cervices were isolated and suspended in organ baths connected to a cervimeter for measurement of the stretch-tension relationship. In a different group of animals, cervical collagen content was measured using light-induced fluorescence in non-pregnant and, longitudinally, in pregnant rats. Cervical resistance and collagen content decreased progressively during pregnancy. The changes in cervical resistance mirrored those in cervical collagen content and the nadir in both occurred about two days prior to the onset of labor. Our study suggests that cervical preparation for delivery does not occur acutely at the time of labor and that cervical collagen content determines cervical resistance.

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“…Of importance, the recruitment and activation of macrophages before labor indicate a role for this immune cell in cervical ripening [19][20][21][22]. Activated macrophages produce prostaglandins and proinflammatory cytokines that stimulate collagenase production by cervical fibroblasts [23][24][25]. Indeed, prostaglandins may directly promote restructuring of the extracellular collagen matrix [26][27][28] as well as act indirectly through vasoactive effects to enhance permeability of blood vessels to leukocytes and fluid [29,30].…”

Section: Introductionmentioning

confidence: 99%