Effect of propolis and N-acetylcysteine supplementation on lipoprotein subclasses distribution and paraoxonase 1 activity in subjects with acute respiratory infection

Vekić, Jelena; Ivanišević, Jasmina; Zeljković, Aleksandra; Spasojević‐Kalimanovska, Vesna; Bogavac-Stanojević, Nataša; Mihajlović, M; Janać, Jelena; Vujčić, Sanja; Miljković, Milica; Zujovic, D.; Kotur‐Stevuljević, Jelena

doi:10.5937/jomb0-24695

Cited by 6 publications

(4 citation statements)

References 29 publications

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“…NAC and propolis are two compounds that have been studied in fixed combination for their potential synergistic effects on oxidative stress and inflammatory or biochemical parameters in acute and chronic respiratory infections [21,22]. However, no data on synergism against bacterial biofilms have been published yet.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Antibiofilm Effect of N-Acetyl-L-cysteine/Dry Propolis Extract Combination on Bacterial Pathogens Isolated from Upper Respiratory Tract Infections

Božić,

Ćirković,

Milovanović

et al. 2023

Pharmaceuticals

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Bacterial biofilms play an important role in the pathogenesis of chronic upper respiratory tract infections. In addition to conventional antimicrobial therapy, N-acetyl-L-cysteine (NAC) and propolis are dietary supplements that are often recommended as supportive therapy for upper respiratory tract infections. However, no data on the beneficial effect of their combination against bacterial biofilms can be found in the scientific literature. Therefore, the aim of our study was to investigate the in vitro effect of N-acetyl-L-cysteine (NAC) and dry propolis extract in fixed combinations (NAC/dry propolis extract fixed combination) on biofilm formation by bacterial species isolated from patients with chronic rhinosinusitis, chronic otitis media, and chronic adenoiditis. The prospective study included 48 adults with chronic rhinosinusitis, 29 adults with chronic otitis media, and 33 children with chronic adenoiditis. Bacteria were isolated from tissue samples obtained intraoperatively and identified using the MALDI-TOF Vitek MS System. The antimicrobial activity, synergism, and antibiofilm effect of NAC/dry propolis extract fixed combination were studied in vitro. A total of 116 different strains were isolated from the tissue samples, with staphylococci being the most frequently isolated in all patients (57.8%). MICs of the NAC/dry propolis extract fixed combination ranged from 1.25/0.125 to 20/2 mg NAC/mg propolis. A synergistic effect (FICI ≤ 0.5) was observed in 51.7% of strains. The majority of isolates from patients with chronic otitis media were moderate biofilm producers and in chronic adenoiditis they were weak biofilm producers, while the same number of isolates in patients with chronic rhinosinusitis were weak and moderate biofilm producers. Subinhibitory concentrations of the NAC/propolis combination ranging from 0.625–0.156 mg/mL to 10–2.5 mg/mL of NAC combined with 0.062–0.016 mg/mL to 1–0.25 mg/mL of propolis inhibited biofilm formation in all bacterial strains. Suprainhibitory concentrations ranging from 2.5–10 mg/mL to 40–160 mg/mL of NAC in combination with 0.25–1 mg/mL to 4–16 mg/mL of propolis completely eradicated the biofilm. In conclusion, the fixed combination of NAC and dry propolis extract has a synergistic effect on all stages of biofilm formation and eradication of the formed biofilm in bacteria isolated from upper respiratory tract infections.

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Section: Discussionmentioning

confidence: 99%

In Vitro Antibiofilm Effect of N-Acetyl-L-cysteine/Dry Propolis Extract Combination on Bacterial Pathogens Isolated from Upper Respiratory Tract Infections

Božić,

Ćirković,

Milovanović

et al. 2023

Pharmaceuticals

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“…No adverse event was recorded during the trial [204]. Other clinical trials on COVID-19 and ear, nose, and throat infections using combination treatments with propolis as one of the bioactive ingredients had also been shown to be efficacious in alleviating symptoms, and more importantly, safe [205][206][207][208].…”

Section: Human Clinical Trialsmentioning

confidence: 95%

Antiviral, Antibacterial, Antifungal, and Antiparasitic Properties of Propolis: A Review

Zulhendri

Chandrasekaran²,

Kowacz

et al. 2021

Foods

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Propolis is a complex phytocompound made from resinous and balsamic material harvested by bees from flowers, branches, pollen, and tree exudates.Humans have used propolis therapeutically for centuries. The aim of this article is to provide comprehensive review of the antiviral, antibacterial, antifungal, and antiparasitic properties of propolis. The mechanisms of action of propolis are discussed. There are two distinct impacts with regards to antimicrobial and anti-parasitic properties of propolis, on the pathogens and on the host. With regards to the pathogens, propolis acts by disrupting the ability of the pathogens to invade the host cells by forming a physical barrier and inhibiting enzymes and proteins needed for invasion into the host cells. Propolis also inhibits the replication process of the pathogens. Moreover, propolis inhibits the metabolic processes of the pathogens by disrupting cellular organelles and components responsible for energy production. With regard to the host, propolis functions as an immunomodulator. It upregulates the innate immunity and modulates the inflammatory signaling pathways. Propolis also helps maintain the host’s cellular antioxidant status. More importantly, a small number of human clinical trials have demonstrated the efficacy and the safety of propolis as an adjuvant therapy for pathogenic infections.

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“…Studies suggest that propolis influences lipoprotein mechanisms, resulting in decreased LDL levels, elevated HDL levels, and fostering cardiovascular protection. Propolis demonstrates its preventive effect against cardiovascular diseases by inhibiting the lipase enzyme while activating the paraxonase enzyme [61,62]. Additionally, propolis has been associated with blood-thinning effects and has been reported to play a role in lowering blood pressure, further contributing to its potential to support cardiovascular health [63][64][65].…”

Section: Propolis and Cardiovascularmentioning

confidence: 99%

A Miracle Food Supplement Obtained from Beehives: Propolis

Kolayli

2024

Herbs and Spices - New Perspectives in Human Health and Food Industry [Working Title]

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In recent years, propolis has garnered substantial global attention as a dietary supplement, owing to its multifaceted nature and diverse biological properties. Derived from beehives, this natural product, characterized by its sticky, waxy consistency, and aromatic scent, is a complex amalgamation of plant and animal origins. Produced by honeybees through the enzymatic processing of resinous substances from herbal extracts, propolis serves an array of structural and functional roles, from insulating beehives to fortifying their defense against diseases. Raw propolis, collected from hives, is harnessed for various formulations as a food supplement following extraction using different solvents. Ethanol (70%) stands as the preferred solvent for propolis extraction, with oil-based and water-based extracts also viable options. Non-toxic propolis extracts are commonly consumed, often encapsulated within natural polymers like chitosan, pectin, alginate, and dextran. Renowned for its diverse array of biological activities, propolis showcases a broad spectrum of functionalities. These encompass antioxidant, antimicrobial, antiviral, anti-inflammatory, anti-tumoral, anti-diabetic, immuno-modulating, hepatoprotective, and neuroprotective properties. This book chapter aims to delve into propolis’ composition and its widespread popularity as a dietary supplement. Furthermore, it will explore the multifaceted impact of propolis on human health.

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Effect of propolis and N-acetylcysteine supplementation on lipoprotein subclasses distribution and paraoxonase 1 activity in subjects with acute respiratory infection

Cited by 6 publications

References 29 publications

In Vitro Antibiofilm Effect of N-Acetyl-L-cysteine/Dry Propolis Extract Combination on Bacterial Pathogens Isolated from Upper Respiratory Tract Infections

In Vitro Antibiofilm Effect of N-Acetyl-L-cysteine/Dry Propolis Extract Combination on Bacterial Pathogens Isolated from Upper Respiratory Tract Infections

Antiviral, Antibacterial, Antifungal, and Antiparasitic Properties of Propolis: A Review

A Miracle Food Supplement Obtained from Beehives: Propolis

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