Effect of Propagation Method and Ploidy Level of Various Rootstocks on the Response of the Common Clementine (Citrus clementina Hort. ex Tan) to a Mild Water Deficit

Abstract

Current climatic upheavals reduce water availability which impacts the growth and fruit quality of plants. In citrus crops, scion/rootstock combinations are used to ensure high fruit production and quality and a stress tolerance/resistance. Our objective was to assess the effect on the clementine scion (C) under natural mild water deficit of (i) polyploid rootstocks by comparing the allotetraploid FlhorAG1 (C/4xFLs; trifoliate orange + Willowleaf mandarin) with its diploid parents, trifoliate orange (C/2xTOs), and Willowleaf mandarin (C/2xWLs), and with a diploid genotype used as reference (Carrizo citrange, C/2xCCs), (ii) rootstock propagation methods by comparing trifoliate orange seedling (C/2xTOs) with cutting (C/2xTOc). A mild water deficit observed under orchard conditions during the summer period (July–August) induced a significant change in yield (except in C/2xTOs), fruit size, and quality. C/2xCCs, C/2xTOs, and C/2xWLs appeared less affected by water deficit as indicated by their lower reduction of predawn leaf water potential (Ψpd), relative water content (RWC), transpiration (E), and photosynthetic parameters (Pnet and gs). Their greater redox balance was probably due to their better antioxidant efficiency. Seedling rootstocks lead to a better adaptation of clementine scions to water deficit than cutting or allotetraploid rootstock. Improving the tolerance to water deficit requires taking into consideration the rootstock genotype, propagation method, and ploidy level.

Tetraploidy Confers Superior in vitro Water-Stress Tolerance to the Fig Tree (Ficus carica) by Reinforcing Hormonal, Physiological, and Biochemical Defensive Systems

“…It could be considered as a significant strategy in which tetraploid genotypes cope with water stress and display extra tolerance to severe water stress conditions compared with their diploid genotypes. Our findings support previous reports that enhanced osmolyte accumulation confers superiority to tetraploid genotypes to better tolerate water stress than diploids (Liu et al, 2011;Zhang et al, 2015;Wei et al, 2019;Oustric et al, 2020).…”

Intermediate Inheritance with Disomic Tendency in Tetraploid Intergeneric Citrus × Poncirus Hybrids Enhances the Efficiency of Citrus Rootstock Breeding

“…Under salinity stress or water deficit, tetraploid seedlings were shown to be more tolerant than their parental diploid counterparts [12][13][14][15][16][17]. Increased tolerance was also observed when rootstock was grafted [18][19][20]. Better tolerance to cold was reported in clementine trees grafted onto tetraploid Carrizo citrange [21].…”