Effect of Prolactin and Bromocriptine on the Population of Prostate Neuroendocrine Cells from Intact and Cyproterone Acetate‐Treated Rats: Stereological and Immunohistochemical Study

Ingelmo, Ildefonso; Gómez, Vicente; Martı́n, Rocı́o; Codesal, J.; Rodríguez, Rosario; Pozuelo, José Manuel; Santamarı́a, Luis

doi:10.1002/ar.20552

Cited by 9 publications

(5 citation statements)

References 52 publications

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“…The effect of CA may not be solely attributable to the androgen blockade, because CA also exerts antigonadotropic action (Raudrant and Rabe, 2003) and even a possible hyperprolactinemic action (Fonzo et al, 1977). These responses of the epithelial population of rat prostate to androgenic blockade and PL treatment, were, in part, different from those observed for other lineages of cells from peripubertal prostate epithelium, as, for instance, the amount of neuroendocrine cells decreases after androgenic blockade but increases with treatment with PL (Ingelmo et al, 2007). Fig.…”

Section: Discussionmentioning

confidence: 82%

Effect of Prolactin on the Population of Epithelial Cells From Ventral Prostate of Intact and Cyproterone Acetate‐Treated Peripubertal Rats: Stereological and Immunohistochemical Study

Gómez

Ingelmo

Martı́n

et al. 2009

The Anatomical Record

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The interactions between steroid and nonsteroid hormones in the prostate are of special interest during the growth phase of the gland. The purpose of this work is to study the influence of prolactin (PL), with or without androgenic blockade, on epithelial cells from peripubertal rat ventral prostate. Twenty male peripubertal Sprague-Dawley rats were grouped as controls, or treated with cyproterone acetate (CA), CA plus PL (CA-PL), or PL. The total number (N total) of epithelial cells, and their labeling indices to proliferative cell nuclear antigen (LI PCNA), apoptosis (LI apoptosis) and androgen receptors (LI AR) were measured. CA and PL treatment significantly decrease the N total, but the LI PCNA was unchanged. We have observed a greater LI apoptosis in pharmacologically castrated animals without PL than in the rats with androgenic blockade with PL. The LI AR does not change with CA treatment in the ventral region, but the PL significantly increases it. Androgenic blockade and PL decrease the number of epithelial cells from the ventral prostate. These changes are not attributable to the decrease of cell proliferation, rather to the increase of epithelial apoptosis. The increase of cells expressing AR after treatment with PL might be attributed to the decrease of testosterone secretion caused by the hyperprolactinemia. PL does not modulate the size of the ventral prostate in prepubertal rats. Anat Rec, 292:746-755, 2009. V V C 2009 Wiley-Liss, Inc.

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Section: Discussionmentioning

confidence: 82%

Effect of Prolactin on the Population of Epithelial Cells From Ventral Prostate of Intact and Cyproterone Acetate‐Treated Peripubertal Rats: Stereological and Immunohistochemical Study

Gómez

Ingelmo

Martı́n

et al. 2009

The Anatomical Record

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“…NE cells in the PZ are unlikely to affect the development of BPH. In previous studies, the investigators often counted the NE cells as a proportion of total epithelial cells [ 12 , 18 , 19 ] but we evaluated them by the density in all cells in the TZ because we considered that NE cells had an effect on not only epithelial cells but also stromal cells in a paracrine fashion [ 17 ]. This is the first attempt to do so.…”

Section: Discussionmentioning

confidence: 99%

Distribution of Neuroendocrine Cells in the Transition Zone of the Prostate

Kyoda

Ichihara

Hashimoto

et al. 2017

Advances in Urology

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Objectives. To evaluate the distribution of neuroendocrine (NE) cells which may influence the development of benign prostatic hyperplasia (BPH) in the transition zone (TZ). Methods. We reviewed specimens from 80 patients who underwent radical prostatectomy in our institution and evaluated the density of NE cells in the TZ. They were histologically classified into 3 groups: those with no adenomatous nodule in the TZ (group A), those with small nodules with normal epithelium and stroma around them in the TZ (group B), and those with large nodules occupying the TZ (group C). In the patients of group B, intra-adenoma (adenomatous nodules) and extra-adenoma (normal tissue) NE cells in the TZ were separately counted. Results. There were 22, 23, and 35 patients in groups A, B, and C, respectively. The median density of NE cells in the TZ of group B patients, 2.80/mm2, was significantly higher than that of NE cells in group A, 1.43/mm2, and group C, 0.61/mm2 (p < 0.001). In group B, the median density of extra-adenoma NE cells was significantly higher than that of intra-adenoma. Conclusions. Many NE cells exist around small adenoma in the TZ. NE cells may influence the initial growth of BPH in a paracrine fashion. Trial Registration. This study approved by our institutional review board was retrospectively registered (#272-14).

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“…CD24 is a potential oncogene reported to be overexpressed in a large variety of human malignancies. 31 We selected the prolactin receptor for further characterization, as this polypeptide growth factor-receptor axis has been implicated in the development of the normal, 32,33 hyperplastic, 34 and neoplastic 35 prostate. We found the prolactin receptor to be overexpressed in ductal versus acinar adenocarcinoma at both the transcript level and at the protein level, as validated by immunohistochemistry.…”

Section: Discussionmentioning

confidence: 99%

“…We found the prolactin receptor to be overexpressed in ductal versus acinar adenocarcinoma at both the transcript level and at the protein level, as validated by immunohistochemistry. Whether the prolactin receptor might affect ductal adenocarcinoma growth to a greater degree than acinar adenocarcinoma is not known, but it is of interest that prolactin promotes ductal morphogenesis 34 and an increase in volume of ductal epithelium 32 in rodent prostates. It should be noted that the prolactin receptor, although overexpressed in ductal compared with acinar adenocarcinoma, does not seem to be practical marker in immunohistochemistry for ductal adenocarcinoma, because of the overlap in immunopositivity in ductal and acinar adenocarcinomas.…”

Section: Discussionmentioning

confidence: 99%

Gene expression profiles of ductal versus acinar adenocarcinoma of the prostate

et al. 2009

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Ductal adenocarcinoma is an uncommon variant of prostatic adenocarcinoma with a generally more aggressive clinical course than usual acinar adenocarcinoma. However, the molecular distinction between ductal and acinar adenocarcinomas is not well characterized. The aim of this investigation was to evaluate the relatedness of ductal versus acinar prostatic adenocarcinoma by comparative gene expression profiling. Archived, deidentified, snap frozen tumor tissue from 5 ductal adenocarcinomas, 3 mixed ductal-acinar adenocarcinomas, and 11 acinar adenocarcinomas cases were analyzed. All cases of acinar and ductal adenocarcinomas were matched by Gleason grade. RNA from whole tissue sections of the 5 ductal and 11 acinar adenocarcinomas cases were subjected to gene expression profiling on Affymetrix U133Plus2 microarrays. Independently, lasercapture microdissection was also performed on the three mixed ductal-acinar cases and five pure acinar cases to isolate homogeneous populations of ductal and acinar carcinoma cells from the same tumor. Seven of these laser-capture microdissected samples (three ductal and four acinar cell populations) were similarly analyzed on U133Plus2 arrays. Analysis of data from whole sections of ductal and acinar carcinomas identified only 25 gene transcripts whose expression was significantly and at least two-fold different between ductal and acinar adenocarcinomas. A similar analysis of microdissected cell populations identified 10 transcripts, including the prolactin receptor, with more significant differences in expression of 5-to 27-fold between ductal and acinar adenocarcinomas cells. Overexpression of prolactin receptor protein in ductal versus acinar adenocarcinoma was confirmed by immunohistochemistry in an independent set of tumors. We conclude that ductal and acinar adenocarcinomas of the prostate are strikingly similar at the level of gene expression. However, several of the genes identified in this study, including the prolactin receptor, represent targets for further investigations on the molecular basis for histomorphological and clinical behavioral differences between acinar and ductal adenocarcinomas.

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Effect of Prolactin and Bromocriptine on the Population of Prostate Neuroendocrine Cells from Intact and Cyproterone Acetate‐Treated Rats: Stereological and Immunohistochemical Study

Cited by 9 publications

References 52 publications

Effect of Prolactin on the Population of Epithelial Cells From Ventral Prostate of Intact and Cyproterone Acetate‐Treated Peripubertal Rats: Stereological and Immunohistochemical Study

Effect of Prolactin on the Population of Epithelial Cells From Ventral Prostate of Intact and Cyproterone Acetate‐Treated Peripubertal Rats: Stereological and Immunohistochemical Study

Distribution of Neuroendocrine Cells in the Transition Zone of the Prostate

Gene expression profiles of ductal versus acinar adenocarcinoma of the prostate

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